A5114803510- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Clinical Laboratory Practices and Quality Control
- Pregnancy and preeclampsia studies
- Reproductive Physiology in Livestock
- Hematological disorders and diagnostics
- Digital Imaging for Blood Diseases
- Sperm and Testicular Function
- Uterine Myomas and Treatments
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Pregnancy and Medication Impact
- Meta-analysis and systematic reviews
- Liver Diseases and Immunity
- Clinical practice guidelines implementation
- Reliability and Agreement in Measurement
- Gynecological conditions and treatments
- Monoclonal and Polyclonal Antibodies Research
- Force Microscopy Techniques and Applications
- Endometrial and Cervical Cancer Treatments
- Cell Adhesion Molecules Research
- Blood Coagulation and Thrombosis Mechanisms
University of Chile
1992-2023
University of California, San Francisco
2022-2023
University of California, Berkeley
2023
Universidad Nacional de Asunción
2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2007-2021
Microgen (United States)
2021
UC Irvine Health
2020
Spanish Society of Hematology and Hemotherapy
2017-2020
Universidad Nacional de Loja
2019
Halitus Instituto Médico
2009-2016
The increased cardiovascular risk present in chronic kidney disease (CKD) is related to the development of endothelial dysfunction, whose mechanisms are still unclear. Accumulation toxins and proinflammatory cytokines may constitute danger-associated molecular patterns (DAMP) which cells continuously exposed. Potential involvement recognizing DAMP, such as TLR inflammasomes, has been explored.Endothelial culture were exposed sera samples collected from patients with CKD: (i) stages 4-5 not...
Trophoblast invasion is a temporally and locally restricted process, which regulates implantation oxygen arrival to the embryo through dialog with spiral artery endothelium. factors angiogenic potential are activated by hypoxia. Their capacities induce proliferation, migration, of trophoblastic cells have been investigated.The expression interleukin (IL)-6, CD126, CD130, vascular endothelial growth factor (VEGF), hypoxia inducible factor-1alpha (HIF-1alpha) has silenced in JEG-3...
Citation Junovich G, Mayer Y, Azpiroz A, Daher S, Iglesias Zylverstein C, Gentile T, Pasqualini Markert UR, Gutiérrez G. Ovarian stimulation affects the levels of regulatory endometrial NK cells and angiogenic cytokine VEGF. Am J Reprod Immunol 2011; 65: 146–153 Problem Endometrial play a critical role in uterine vascularization producing factors. Impact ovarian on expression VEGF normal fertile oocyte donors effect injury treatment these parameters have been investigated. Method study...
Abstract Problem The aim of this study was to investigate the possible relationship between vaginal/rectal microbiome disbalances and miRNA expression with infertility. Method Observational, exploratory, preliminary study. A total 287 multiple IVF failure infertile patients were recruited. Twenty fertile women, not failure, recruited as control group. Swab samples collected from vagina rectum. Microbial composition by NGS real‐time PCR vaginal rectal measured. Immunometabolic markers blood...
In this study, we have evaluated the relationship between acrosome reaction-inducing activity of individual human follicular fluid samples and their steroid content. Eighteen were obtained during egg retrieval in six patients undergoing assisted fertilization. Motile spermatozoa incubated modified Tyrode's medium (26 mg/ml bovine serum albumin) for 20 h at 1 x 107 cells/ml. a single experiment, aliquots semen specimen simultaneously treated with an aliquot each sample. The percentage reacted...
ProblemPeripheral counts of CD16 + NK cells have been well characterized in reproductive failure.However, not enough case-control clinical studies conducted to establish normal or abnormal +/À values the endometrium. Method studyPeripheral and endometrial cell by FACS, IL-6, VEGF cytokines levels ELISA were fertile women unexplained infertility patients with implantation failures (UI-IF) during window.ROC correlation analysis performed. ResultsReceiver Operating Characteristic(ROC) revealed...
Canellada A, Färber Zenclussen AC, Gentile T, Dokmetjian J, Keil Blois S, Miranda Berod L, Gutiérrez G, Markert UR, Margni RA. Interleukin regulation of asymmetric antibody synthesized by isolated placental B cells. AJRI 2002; 48: 275–282 © Blackwell Munksgaard, 2002 PROBLEM: Protecting antibodies against trophoblast surface molecules were previously described. Here we analysed the synthesis IgG B‐lymphocytes. METHOD OF STUDY: cells from human term placenta and cord blood, stimulated with...
Integrin mechanosensing plays an instrumental role in cell behavior, phenotype, and fate by transmitting mechanical signals that trigger downstream molecular cellular changes. For instance, force transfer along key amino acid residues can mediate adhesion. Disrupting binding sites within α5β1 integrin’s partner, fibronectin (FN) diminishes adhesive strength. While past studies have shown the importance of these adhesion, relationship between dynamics how integrin distributes across surface...
Problem Regulatory factors seem to be essential achieve transition from implantation window placental vascularization. A novel function of interleukin (IL)‐6 in the promotion Th2 differentiation and inhibition Th1 polarization has been demonstrated. Considering that response promotes antibody synthesis, we postulate IL‐6 could modulating quality this during pregnancy by increasing proportion blocking asymmetric antibodies. Method study We investigated expression blocking‐asymmetric‐IgG CBA/J...
Problem: CBA/J × DBA/2 abortion rate could be the consequence of a deficient local production T helper (Th2) cytokines, which cause fetal wastage via fgl2 prothrombinase. Heparin reduces significantly in mice and recurrent spontaneous (RSA) patients. We proposed to determine effect enoxaparin on levels interleukin (IL)‐6 during murine pregnancy. Method study: Recombinant human IL‐6 (rhIL‐6) or were inoculated pregnant days 6.5–12.5. sera as well culture supernatants day 9.5 fetoplacental...
The Spanish haematology external quality assessment scheme (EQAS), established in 1984, is run by the Haematology and Haemotherapy Association (AEHH) [Quality Assurance Health Care 3 (1991) 75] functions to evaluate reproducibility of diagnostic samples clinical laboratories. Hospital Clinic University Barcelona (HCB) serves as EQAS Coordination Centre follows guidelines International Committee for Standardization [Annali dell'Istituto superiore di Sanità 31 (1995) 95; Journal Hematology 68...
Problem Several studies indicate that RANTES (regulated on activation, normal T cell expressed and secreted) is able to downregulate T‐cell responses which suggest it might be relevant for fetal tolerance induction. However, the role of in pregnancy had not been established. Here we investigate regulation during early potential failures leading losses pregnancies. Method study progesterone levels were determined sera feto‐placental units from high resorption rate CBA/J × DBA/2 pregnant...
The Abbott (R) Cell-Dyn 3500 (Abbott CD 3500, Diagnostics Division, Mountain View, CA) is a fully automated hematology analyzer capable of providing complete blood count (CBC) profile, including five-part differential leukocyte (DLC) and flagging system in this study. CBC profile DLC the were evaluated according to HA-20 protocol National Committee for Clinical Laboratory Standards (NCCLS) compared Technicon H*2 currently used authors' laboratory. Linearity, carryover, precision, stability...
Pregnancy is personally special to every woman expecting a child, but also interesting from the perspective of an immunologist. During physiological pregnancy, mother's immune system decides tolerate and foster incorporated, non-self,
Considering the potential adverse effects of anticoagulation in abortion treatment, we investigate whether antioxidants might exert same immunoprotection. Although fertility properties Vitamin E have been associated with its antioxidant capacity, effect on cytokine balance during pregnancy is still unknown.Pregnant females from CBA/J × DBA/2 model were orally supplemented or inoculated intraperitoneally enoxaparin. Foeto-placental units scored at 14.5 days pregnancy, and rate was calculated....
Blood smear analysis is a well-known technique in medical laboratories. The clinical relevance of this and its interpretation are very important. Consequently, monitoring laboratory performance by an external quality assessment scheme strongly recommended. This article represents practical guidance document for External Quality Assessment Scheme (EQAS) organizers setting up blood schemes. In the first part guidelines, Hematology Working Group European Committee Assurance Programmes...
Abstract Introduction The Spanish Haematology and Haemotherapy Society organizes peripheral blood smear review scheme , focused on the evaluation of diagnostic proficiency participants by cell morphology analysis. objective was to evaluate efficacy this as an educational tool improve participants. Methods During 2011‐2019, 54 smears, alongside with patient details such age, sex, counts relevant clinical information, were sent average 125 ± 13 laboratories per year. A number 44 shipments...