Oxymatrine, a quinolizidine alkaloid isolated from the traditional Chinese herb Sophora flavescens Aiton, has been demonstrated to exert anti‑inflammatory and atherosclerotic effects, but molecular mechanism yet be elucidated. Accumulating evidence indicates an important role of NLR family pyrin domain containing 3 (NLRP3) inflammasome‑mediated pyroptosis in pathogenesis atherosclerosis. The present study was undertaken investigate whether oxymatrine attenuates oxidized low‑density...

Abstract Background The lesser grain borer ( Rhyzopertha dominica ), a worldwide primary pest of stored grain, causes serious economic losses and threatens food safety. R. can respond to changes in temperature, especially the adaptability heat. In this study, transcriptome analysis exposed different temperatures was performed elucidate differences gene expression underling molecular mechanism. Results Isoform-sequencing generated 17,721,200 raw reads yielded 20,416 full-length transcripts. A...

A series of novel triazone derivatives containing aldehyde hydrazone or ketone moieties were designed, synthesized and their biological activities investigated against Aphis craccivora, Culex pipiens pallens, Helicoverpa armigera, Ostrinia nubilalis, Mythimna separata 14 Kinds fungi. Most the exhibited excellent insecticidal A. craccivora. In particular, aphicidal compounds 3t (35%) 3w (30%) equivalent to pymetrozine at 5 mg/kg. The 3p, 3u, 3y, 5g, 5i, 5l, 5q 5u C. pallens higher than that...

The mechanism underlying oil synthesis in plant fruits remains elusive, as sugar metabolism provides the essential carbon skeleton without a clear understanding of its intricate workings. transcriptome and metabolites' content Symplocos paniculate, an extraordinary with immense ecological significance, were subjected to comparative analysis throughout fruit development. findings unveiled that impact on varied distinct stages Remarkably, during initial phase development from 10 90 days after...

Phosphorus deficiency poses a significant challenge to plant growth and development, particularly in red soil. To alleviate this limitation, phosphorus-solubilizing bacteria (PSB) play crucial role by converting insoluble phosphates present the soil into soluble forms that are accessible plants. Cornus wilsoniana Wangerin is representative oil crop cultivated soil, holding prominent position within China's forestry economic system. Consequently, it essential develop highly stable microbial...

We screened a library of 117 bisphosphonates for antibacterial activity against Escherichia coli. The most potent growth inhibitors where the N-[methyl(4-phenylalkyl)]-3-aminopropyl-1-hydroxy-1,1-bisphosphonates, known bone resorption inhibitors, and there was generally good correlation between cell inhibition E. coli farnesyl diphosphate synthase (FPPS) inhibition. However, some FPPS had no in inhibition, based on result Catalyst pharmacophore modeling, this could be attributed to...

Abstract Pharmacological activities of some natural products diminish and even disappear after purification. In this study, we explored the mechanisms underlying decrease acute oral toxicity Coptidis Rhizoma extract The water solubility, in vitro absorption plasma exposure berberine (the major active compound) were much better than those pure berberine. Scanning electron microscopy, laser scanning confocal microscopy (LSCM) dynamic light scattering experiments confirmed that nanoparticles...

High concentrations of PEG400 increase <italic>in vivo</italic> exposure to berberine (D) by increasing its solubility (A), permeability (B), and lymphatic transport (C).

Gardenia jasminoides fruits are extensively grown worldwide, with a large harvest, and its major medicinal ingredients geniposide crocins. Research on their accumulation biosynthsis-related enzymes is rare. In this study, the of crocin G. at different developmental stages were clarified by HPLC. The highest cumulative amount was 2.035% during unripe-fruit period, content 1.098% mature-fruit period. Furthermore, transcriptome sequencing performed. A total 50 unigenes encoding 4 key related in...

The optimization of explant selection and adjustment plant growth regulators (PGRs) ratio may enhance the efficiency micro-propagation Gardenia jasminoides Ellis. findings study suggest that shoot tip proved to be optimal for regenerating adventitious buds, with an impressive regeneration rate 77.78% average number buds being 2.86. ideal medium consisted Murashige Skoog (MS) supplemented 6-benzylaminopurine (6-BA) at a 2 mg L−1, indoleacetic acid (IAA) 0.2 kinetin (KT) 0.15 resulting in...

Crocins are highly valuable medicinal compounds for treating human disorders, and they also serve as spices coloring agents. However, the supply of crocins from plant extractions is insufficient current demands, using synthetic biology to produce remains a big challenge. Here, we report in vivo production five types E. coli with GjUGT94E13 GjUGT74F8, which responsible glycosylation crocetin, crocin-producing Gardenia jasminoides. Subsequently, native UDP-glucose biosynthesis strengthened by...

Land salinization considerably limits crop production. Biological improvement of saline and alkaline land is an important way to achieve efficient use. It crucial study the salt tolerance halophyte resources in order explore improve plant through biological improvement. Glaux maritima a mesophyte with strong tolerance. In this study, we conducted research on mechanism G. phenotypic, physiological, transcriptomic aspects. The results indicate that leaf cross-sections revealed has gland tissue...

Abstract Based on the broad-spectrum biological activities of echinopsine and acylhydrazones, a series derivatives containing acylhydrazone moieties have been designed, synthesized their were evaluated for first time. The bioassay results indicated that most compounds showed moderate to good antiviral against tobacco mosaic virus (TMV), among which ( I ) (inactivation activity, 49.5 ± 4.4%; curative 46.1 1.5%; protection 42.6 2.3%) its 1 44.9 4.6%; 39.8 2.6%; 47.3 4.3%), 3 47.9 0.9%; 43.7...

One new flavanonol 4 H-​1-​benzopyran-​4-​one,2-​(4-​hydroxyphenyl)​-​3,​7-​dihydroxy-​5-​methoxy-​8-​[5-​methyl-​2-​(1-​methylethenyl)​-​4-​hexenyl]​(21), and twenty-six known compounds 1-20, 22-27 were isolated from the dried root of Sophora flavescens (S. flavescens) in this chemical study. Their structures elucidated according to spectroscopic spectrometric methods. All evaluated for their cytotoxicity against lung cancer A549 cells colon HCT116 cells. Among them, compound 21 showed...

Artemisinic acid, an amorphane sesquiterpene, is isolated from Artemisia annua L. Although having less efficacy than artemisinin, artemisinic acid has a variety of pharmacological activity, such as antimalarial anti-tumor antipyretic effect, antibacterial allelopathy effect and anti-adipogenesis effect. This development drastically increased demand worldwide. many approaches, namely extraction A.annua L, in vitro production by cell tissue culture, total chemical synthesis fermentation use...

Abstract Rhododendron molle G.Don is a well‐known traditional medicine which has been used to treat rheumatic inflammation. In this study, an inflammatory model of lipopolysaccharide (LPS)‐stimulated RAW264.7 cells was established analyze the anti‐inflammatory effect and potential mechanism methanol extract R. leaves (RLE). The production NO expression tumor necrosis factor by LPS were detected Griess reaction enzyme linked immunosorbent assay (ELISA). mRNA TNF‐α , IL‐1β IL‐6 COX‐2 iNOS...

Abstract 2,3-Fused indoles are found in numerous natural products and drug molecules. Although several elegant methods for the synthesis of this structural motif have been reported, long reaction times harsh conditions sometimes required yields tend to be low. Herein, we report a microwave method straightforward access various types 2,3-fused via AgSbF 6 -catalysed intramolecular difunctionalization o -alkynylanilines. played role both hydroamination step imine-formation step. This method,...

On the basis of sulfonamide moieties widely used in drugs and mode action pymetrozine structures some transient receptor potential channel antagonists to improve insecticidal activities triazone insecticides, a series derivatives containing or sulfonimide were designed, synthesized, characterized. The bioassay results showed that most these exhibited excellent against Aphis craccivora. Especially, (substituted)phenylsulfonylamino triazones I-9 (LC50 = 2.9869 mg/kg), I-10 3.4957 I-11 2.3154...

Symplocos paniculate is an oil plant exhibiting tissue-specific variations in content and fatty acid composition across the whole fruit (mainly pulp seed). And its synthesis intricately linked to accumulation transformation of sugars. Nevertheless, there remains a dearth understanding regarding how sugar metabolism impacts S. fruit. To unravel intricate mechanism underlying impact on lipid biosynthesis paniculata fruit, comparative analysis was conducted transcriptome metabolite seed...

Pharmacokinetic studies are crucial for elucidating the effective constituents and formula compatibility of traditional Chinese medicines (TCMs). However, have usually been limited to single dosages detection systemic blood concentrations. To obtain comprehensive pharmacokinetic information, here we propose a multi-dosage multi-sampling (blood from portal vein or circulation, liver) strategy comparatively study pharmacokinetics multi-form TCMs, i.e., pure constituents, TCM extracts. Based on...

Symplocos paniculata are reported to exhibit seed dormancy, which impedes its cultivation and widespread adoption. In this study, a comprehensive method was established overcome dormancy by subjecting seeds scarification in 98% H2SO4 for 10 min, followed 1000 mg·L−1 GA3 soaking 48 h stratification at 4 °C 100 days. The germination percentage has increased significantly, peak of 42.67%, though the could not germinate timely NaOH scarification. Additionally, dynamic changes key stored...