A5078639626- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Retinoids in leukemia and cellular processes
- Cell Adhesion Molecules Research
- Retinal Diseases and Treatments
- Receptor Mechanisms and Signaling
- Ocular Oncology and Treatments
- Corneal Surgery and Treatments
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Force Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Biotin and Related Studies
- Surfactants and Colloidal Systems
- Molecular Junctions and Nanostructures
- Advanced Fluorescence Microscopy Techniques
- Ubiquitin and proteasome pathways
- Polymer Surface Interaction Studies
- Protein Kinase Regulation and GTPase Signaling
- Monoclonal and Polyclonal Antibodies Research
- Molecular Sensors and Ion Detection
- Biochemical and Molecular Research
Université Laval
2014-2025
Hôpital du Saint-Sacrement
2011-2024
PROTEO
2013-2019
Centre hospitalier de l'Université Laval
2003-2016
Centre hospitalier universitaire de Québec
2002-2011
York University
2008
Université de Limoges
2005-2006
Centre National de la Recherche Scientifique
1998-2006
Université de Rennes
2006
Laboratoire de Chimie
2005
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteraction between biotin lipids and streptavidin in monolayers: formation of oriented two-dimensional protein domains induced by surface recognitionR. Blankenburg, P. Meller, H. Ringsdorf, C. SalesseCite this: Biochemistry 1989, 28, 20, 8214–8221Publication Date (Print):October 1, 1989Publication History Published online1 May 2002Published inissue 1 October...
Phospholipase A 2 , a ubiquitous lipolytic enzyme that actively catalyses hydrolysis of phospholipids, has been studied as model for enzyme‐substrate reactions, membrane structural probe, and lipid‐protein interactions. Its mechanism action remains largely controversial. We report here the first time direct microscopic observation fluorescently marked phospholipase ( Naja naja ) against phosphatidylcholine monolayers in lipid phase transition region. Under these conditions, is shown to...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEllipsometric study of the physical states phosphatidylcholines at air-water interfaceDaniel. Ducharme, Jean Joseph. Max, Christian. Salesse, and Roger M. LeblancCite this: J. Phys. Chem. 1990, 94, 5, 1925–1932Publication Date (Print):March 1, 1990Publication History Published online1 May 2002Published inissue 1 March 1990https://pubs.acs.org/doi/10.1021/j100368a038https://doi.org/10.1021/j100368a038research-articleACS PublicationsRequest reuse...
The binding of peripheral proteins to membranes results in different biological effects. large diversity membrane lipids is thought modulate the activity these proteins. However, information on selective still largely lacking. Lipid monolayers at air/water interface are useful model systems for studying parameters responsible protein binding. We have thus measured maximum insertion pressure (MIP) two from photoreceptors, Retinitis pigmentosa 2 (RP2) and recoverin, estimate their lipid...
Membrane binding of monotopic proteins can involve various post-translational modifications or a combination some membrane-binding elements. For example, amphipathic α helices and palmitoylation could drive the membrane attachment proteins. G-protein-coupled receptor kinases (GRKs) regulate activity receptors. Several members family GRKs are acylated. Moreover, C-terminus GRK6 contains an helix palmitoyl group, which also be case for GRK4 isoforms. In our experiments, GRK4α/β-derived...
Langmuir monolayers were used to characterize the influence of physical state phospholipid on binding protein Retinis Pigmentosa 2 (RP2). The parameters RP2 (maximum insertion pressure (MIP), synergy and ΔΠ0) in thus analyzed presence phospholipids bearing increasing fatty acyl chain lengths at temperatures where their liquid-expanded (LE), liquid-condensed (LC), or solid-condensed (SC) states can be individually observed. data show that a larger value is observed LC/SC than LE state,...
Abstract The visual photoreception takes place in the retina, where specialized rod and cone photoreceptor cells are located. outer segments contain a stack of 500–2,000 sealed membrane disks. Rhodopsin is pigment located segment disks, it member G‐protein‐coupled receptor (GPCR) superfamily, an important group proteins responsible for majority physiological responses to stimuli such as light, hormones, peptides, etc. Alongside rhodopsin, peripherin/Rom disk rims thought be morphology. Here...
The accumulation of fibronectin (FN) in response to corneal epithelium injury has been postulated turn on expression the FN-binding integrin α5β1. In this work, we determined whether activity directed by α5 gene promoter can be modulated FN rabbit epithelial cells (RCEC). driven chloramphenicol acetyltransferase/α5 promoter-bearing plasmids was drastically increased when transfected into RCEC grown FN-coated culture dishes. sequence mediating responsiveness shown bear a perfect inverted...
Summary Metastasis of tumor cells to distant organs is the leading cause death in melanoma. Yet, mechanisms metastasis remain poorly understood. One key question whether all a primary are equally likely metastasize or subpopulations preferentially give rise metastases. Here, we identified subpopulation uveal melanoma expressing multidrug resistance transporter ABCB1 that highly metastatic compared − bulk cells. + also exhibited enhanced clonogenicity, anchorage‐independent growth,...
Abstract A Ca 2+ ‐responsive artificial selenoenzyme was constructed by computational design and engineering of recoverin with the active center glutathione peroxidase (GPx). By combining recognition capacity for (GSH) substrate steric orientation catalytic selenium moiety, engineered selenium‐containing exhibits high GPx activity catalyzed reduction H 2 O (GSH). Moreover, can be switched on/off ‐induced allosterism protein recoverin. This also displays excellent antioxidant ability when it...
Monomolecular films of the membrane protein rhodopsin have been investigated in situ at air−water interface by polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) and X-ray reflectivity order to find conditions that retain secondary structure. The spreading 0 or 5 mN m-1 followed a 30 min incubation time 21 °C resulted unfolding rhodopsin, as evidenced from large increase its molecular area, small monolayer thickness, extensive formation β-sheets expense α-helices...
In this paper, we report the monolayer behavior of pure (5-n-hexadecanoylamino)fluorescein (HDFL) at air−water interface. The spectroscopic characteristics HDFL films interface and mixed Langmuir−Blodgett (LB) palmitic acid (PA) deposited on quartz substrates have been studied compared with in solution. absorption emission spectra were similar to those polar, protic solvents but completely different aprotic, apolar solvents. Detailed studies indicate that fluorescein moieties exist as...