A5084806170- Ocular Oncology and Treatments
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Retinal Development and Disorders
- Chromatin Remodeling and Cancer
- Immunotherapy and Immune Responses
- melanin and skin pigmentation
- Cell Adhesion Molecules Research
- Corneal Surgery and Treatments
- Nanoplatforms for cancer theranostics
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- Extracellular vesicles in disease
- Retinal Diseases and Treatments
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- interferon and immune responses
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Ocular Disorders and Treatments
- Peptidase Inhibition and Analysis
- Hedgehog Signaling Pathway Studies
- Skin Protection and Aging
- Retinal Imaging and Analysis
- Cutaneous Melanoma Detection and Management
Université Laval
2016-2025
Hôpital du Saint-Sacrement
2011-2024
Centre hospitalier universitaire de Québec
2018-2023
Fondation ARC pour la Recherche sur le Cancer
2018
Centre hospitalier de l'Université Laval
2008-2017
Fonds de Recherche du Québec - Santé
2014
Washington University in St. Louis
2009-2013
Research Institute in Oncology and Hematology
2004
Université du Québec à Trois-Rivières
2002-2003
Metastasis is responsible for the death of most cancer patients, yet few therapeutic agents are available which specifically target molecular events that lead to metastasis. We recently showed inactivating mutations in tumor suppressor gene BAP1 closely associated with loss melanocytic differentiation uveal melanoma (UM) and The purpose this study was identify reverse phenotypic effects UM.In silico screens were done compounds predicted differentiate UM cells using Gene Set Enrichment...
Uveal melanoma (UM) is the most common intraocular tumor in adults, arises either de novo from normal choroidal melanocytes (NCMs) or pre-existing nevi that stem NCMs and are thought to harbor UM-initiating mutations, commonly GNAQ GNA11. However, there no commercially available NCM cell lines, nor a detailed protocol for developing an oncogene-mutated CM line (MutCM) study UM development. This aimed establish characterize premalignant models human donor eyes recapitulate populations at...
Uveal melanoma (UM) is an eye cancer that fatal upon metastasis to the liver. Most treatments trialed in UM fail provide therapeutic benefit, thus there urgent need for novel treatment strategies. The MAPK and PI3K signaling pathways, key molecular drivers found be hyper-activated UM, converge on MNK1/2-eIF4E mTORC1/2-4EBP axes. Here, we demonstrate pharmacologic inhibition of MNK1/2 combination with mTOR inhibitor impairs clonogenic outgrowth cell invasion. Proteomic analyses reveal...
Purpose: Development of liver metastasis remains the most common cause mortality in uveal melanoma (UM). A few cell lines cultured from primary UM tumors have been used widely to investigate pathobiology UM. However, translation basic knowledge clinic for treatment metastatic disease has remained incremental at best. In this study, we examined whether properties various passages were similar their corresponding tumors. Methods: Gene expression profiling by microarray was performed on and...
Purpose.: Overexpression of hypoxia inducible factor-1 α (HIF-1α) has been found in several cancers and is thought to correlate with aggressive disease. The purpose our study was investigate the influence HIF-1α on clinical outcome uveal melanoma (UM) along proliferative (MIB-1) vascular (CD31, VEGF-A) markers. Methods.: A retrospective analysis carried out UM tumors from 88 patients. HIF-1α, MIB-1, CD31, VEGF-A expression, as well necrosis, were assessed by immunohistochemistry...
In brachytherapy (BT), or internal radiation therapy, cancer is treated by radioactive implants. For instance, episcleral plaques (EPs) for the treatment of uveal melanoma, are designed according to generic population approximations. However, more personalized implants can enhance precision through better adjustment dose profiles contours cancerous tissues. An original approach integrating biomedical imaging, 3D printing, radioactivity painting, and developed as a workflow development tumor...
Summary Metastasis of tumor cells to distant organs is the leading cause death in melanoma. Yet, mechanisms metastasis remain poorly understood. One key question whether all a primary are equally likely metastasize or subpopulations preferentially give rise metastases. Here, we identified subpopulation uveal melanoma expressing multidrug resistance transporter ABCB1 that highly metastatic compared − bulk cells. + also exhibited enhanced clonogenicity, anchorage‐independent growth,...
Purpose: Epigenetic alterations in uveal melanoma (UM) are still neither well characterized, nor understood. In this pilot study, we sought to provide a deeper insight into the possible role of epigenetic pathogenesis UM and their potential prognostic relevance. To aim, comprehensively profiled histone post-translational modifications (PTMs), which represent features regulating chromatin accessibility gene transcription, formalin-fixed paraffin-embedded (FFPE) tissues, control cell lines,...
Uveal melanoma (UM) is the most common primary intraocular tumor and often spreads to liver. Intercellular communication though extracellular vesicles (EVs) plays an important role in several oncogenic processes, including metastasis, therapeutic resistance, immune escape. This study examines how EVs released by UM cells modify stellate endothelial microenvironment. The surface markers, concentration size of derived from or choroidal melanocytes were characterized high-resolution flow...
Metastatic uveal melanomas are highly resistant to all existing treatments. To address this critical issue, we performed a kinome-wide CRISPR-Cas9 knockout screen, which revealed the LKB1-SIK2 module in restraining melanoma tumorigenesis. Functionally, LKB1 loss enhances proliferation and survival through SIK2 inhibition upregulation of sodium/calcium (Na
Uveal melanoma (UM) is a malignant intraocular tumor that spreads to the liver in half of cases. Since hepatic cells could play role therapeutic resistance metastatic UM, purpose our study was investigate pro-invasive stellate (HSteCs) UM at micro- and macro-metastatic stages. We first performed an immunostaining with alpha-smooth muscle actin (αSMA) localize activated HSteCs macro-metastases from four patients. Their accumulation collagen assessed Masson's Trichrome stain. Next, we...
Cancer aggressiveness is related to the ability of cancer cells escape anchorage dependency toward extracellular matrix, a process regulated by integrin α5β1 and its ligand fibronectin. Here, we characterized expression α5 gene in human uveal melanoma cell lines with distinct tumorigenic properties investigated some mechanisms underlying variations their malignancy. Strong weak was observed no (T108/T115) high (T97/T98) properties, respectively. Expression DNA binding transcription factors...