A5020266152- Ion channel regulation and function
- Ion Channels and Receptors
- Nicotinic Acetylcholine Receptors Study
- Neuroscience of respiration and sleep
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Heme Oxygenase-1 and Carbon Monoxide
- Lipid Membrane Structure and Behavior
- Ion Transport and Channel Regulation
- Nitric Oxide and Endothelin Effects
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- Fatty Acid Research and Health
- Toxin Mechanisms and Immunotoxins
- Lipid metabolism and biosynthesis
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Computational Drug Discovery Methods
- Antimicrobial Peptides and Activities
- Prostate Cancer Treatment and Research
- Protease and Inhibitor Mechanisms
- 3D Printing in Biomedical Research
- Hemoglobin structure and function
Inserm
2016-2025
Université de Tours
2016-2025
Foie, Métabolisme, Cancer
2016-2024
Clinical Investigation Center Plurithematic Tours
2006-2024
Centre de recherche Translationnelle en Médecine moléculaire
2017-2023
Cancéropôle Grand Ouest
2016-2019
Sorbonne Université
2013
Centre Hospitalier Universitaire de Tours
2013
Assistance Publique – Hôpitaux de Paris
2013
Centre National de la Recherche Scientifique
1996-2013
The SK3 channel, a potassium was recently shown to control cancer cell migration, critical step in metastasis outgrowth. Here, we report that expression of the channel markedly associated with bone metastasis. constitutive Ca(2+) entry and migration through an interaction Orai1. We found triggers association Orai1 within lipid rafts. This localization SK3-Orai1 complex seemed essential migration. suggests formation this rafts is gain-of-function, because showed none individual proteins were...
Glioblastoma multiforme (GBM) is the most lethal and common malignant human brain tumor. The intrinsic resistance of highly invasive GBM cells to radiation- chemotherapy-induced apoptosis accounts for generally dismal treatment outcomes. This study investigated ophiobolin A (OP-A), a fungal metabolite from Bipolaris species, its promising anticancer activity against exhibiting varying degrees proapoptotic stimuli. We found that OP-A induced marked changes in dynamic organization F-actin...
// Maxime Guéguinou 1, 9 , Thomas Harnois 2, David Crottes 3 Arnaud Uguen 6, 7 Nadine Deliot Audrey Gambade 1 Aurélie Chantôme Jean Pierre Haelters 4, Paul Alain Jaffrès Marie Lise Jourdan 10 Günther Weber Olivier Soriani 8 Philippe Bougnoux 9, Mignen Nicolas Bourmeyster Bruno Constantin Thierry Lecomte 5, Christophe Vandier * Potier-Cartereau INSERM UMR 1069, Université de Tours, France 2 Equipe ERL 7368, CNRS, Poitiers, Department of Physiology, University California, San Francisco, CA,...
Abstract Potassium channels have been involved in epithelial tumorigenesis but the role of small-conductance Ca2+-activated K+ is unknown. We report here that are expressed a highly metastasizing mammary cancer cell line, MDA-MB-435s. Patch-clamp recordings showed typical channel–mediated currents sensitive to apamin, 4-aminopyridine, and tetraethylammonium. Moreover, cells displayed high intracellular calcium concentration, which was decreased after 24 hours apamin treatment. By regulating...
Background: Colorectal cancer (CRC) is a highly devastating cancer. Ca2+-dependent channels are now considered key regulators of tumor progression. In this study, we aimed to investigate the association non-voltage gated Ca2+ and potassium (KCa) with CRC using transcriptional profile their genes. Methods: We selected total 35 genes covering KCa KCNN1–4, KCNMA1 subunits KCNMB1–4, endoplasmic reticulum (ER) calcium sensors STIM1 STIM2, ORAI1–3 family cation TRP (TRPC1–7, TRPA1, TRPV1/2,4–6...
Edelfosine is an inhibitor of SK3 channel – mediated cell migration. However, this compound bears adverse in vivo side effects. Using dependent cell-migration assay, patch-clamp, 125I-apamin binding, and experiments we tested the ability 15 lipid derivatives with chemical structures inspired from edelfosine to inhibit channels. a structure-activity relationship approach identified analog named Ohmline (1-O-hexadecyl- 2-O-methyl-sn-glycero-3-lactose) potent inhibitory effects on channel. Its...
<h3>Objective:</h3> To report that homoplasmic deleterious mutations in the mitochondrial DNA <i>MT-ATP6/8</i> genes may be responsible for acute episodes of limb weakness mimicking periodic paralysis due to channelopathies and dramatically responding acetazolamide. <h3>Methods:</h3> Mitochondrial sequencing restriction PCR, oxidative phosphorylation functional assays, reactive oxygen species metabolism, patch-clamp technique cultured skin fibroblasts. <h3>Results:</h3> Occurrence a typical...
Glutamate-induced excitotoxicity is a major contributor to motor neuron degeneration in the pathogenesis of amyotrophic lateral sclerosis (ALS). The spinal cord × Neuroblastoma hybrid cell line (NSC-34) often used as bona fide cellular model investigate physiopathological mechanisms ALS. However, physiological response NSC-34 glutamate remains insufficiently described. In this study, we evaluated relevance differentiated (NSC-34D) an vitro for studies. NSC-34D showed morphological and...
// Audrey Gambade 1, * , Sami Zreika 2, Maxime Guéguinou 3 Igor Chourpa 4 Gaëlle Fromont 3, 5 Ana Maria Bouchet Julien Burlaud-Gaillard Marie Potier-Cartereau Sébastien Roger 1 Vincent Aucagne 6 Stéphan Chevalier Christophe Vandier Caroline Goupille 5, Günther Weber 4, Inserm, UMR1069, Nutrition, Croissance et Cancer, Tours, France 2 Department of Medical Lab Technology, Jinan University, Tripoli, Lebanon Ion channel network Canceropole Grand Ouest Université François Rabelais, CHRU Hôpital...
Background Advanced epithelial ovarian cancer (EOC) patients often receive neoadjuvant platinum-based chemotherapy (NAC), with interval surgery (after three cycles of chemotherapy) considered as a major prognostic factors. We examined how changes in body composition (muscle and adipose tissue) during NAC influence prognosis. Objective Using CT images acquired before cohort women advanced EOC, the aim this study was to analyze fat mass) see whether these parameters, at diagnosis or they...
Objective: We tested the hypothesis that inhalation of a low concentration exogenous carbon monoxide (CO) attenuates development hypoxic pulmonary artery hypertension by activation large-conductance voltage and Ca2+-activated K+ channels (BKCa). Methods: The BKCa activity was measured using whole-cell inside-out patch clamp recordings in Wistar rat (PA) myocytes. Pulmonary pressures were vivo membrane potentials recorded vitro pressurized resistance arteries. Results: Chronic CO slightly...
The 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine (edelfosine) is an ether-linked phospholipid with promising anti-cancer properties but some side effects that preclude its full clinical therapeutic exploitation. We hypothesized this lipid could interact plasma membrane ion channels and modulate their function.
Cystic fibrosis (CF) is an inherited disease associated with chronic severe lung inflammation, leading to premature death. To develop innovative anti-inflammatory treatments, we need characterize new cellular and molecular components contributing the mechanisms of inflammation. Here, focused on potential role “transient receptor vanilloid-4” (TRPV4), a nonselective calcium channel. We used both in vitro vivo approaches demonstrate that TRPV4 expressed airway epithelial cells triggers...
The composition of periprostatic adipose tissue (PPAT) has been shown to play a role in prostate cancer (PCa) progression. We recently reported an inverse association between PCa aggressiveness and elevated PPAT linoleic acid (LA) eicosapentaenoic (EPA) content. In the present study, we identified new signaling pathway with positive feedback loop epithelial-to-mesenchymal transition (EMT) transcription factor Zeb1 Ca2+-activated K+ channel SK3, which leads amplification Ca2+ entry cellular...