A5051222833- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Sperm and Testicular Function
- Genetics, Aging, and Longevity in Model Organisms
- Single-cell and spatial transcriptomics
- Apelin-related biomedical research
- Genomics and Chromatin Dynamics
- Nuclear Receptors and Signaling
- Lipid metabolism and disorders
Southern Medical University
2017-2024
Abstract Mammalian male germ cell development is a stepwise cell-fate transition process; however, the full-term developmental profile of cells remains undefined. Here, by interrogating high-precision transcriptome atlas 11,598 covering 28 critical time-points, we demonstrate that from mitotic to post-mitotic primordial accompanied transcriptome-scale reconfiguration and transitional state. Notch signaling pathway essential for initiating arrest maintenance cells’ identities. Ablation HELQ...
Abstract Type 2 diabetes mellitus is one of the most prevalent metabolic diseases presenting with systemic pathologies, including reproductive disorders in male diabetic patients. However, molecular mechanisms that contributing to spermatogenesis dysfunction patients have not yet been fully elucidated. Here, we perform STRT-seq examine transcriptome patients’ testes at single-cell resolution all major cell types testis. Intriguingly, whereas appears largely preserved, gene expression...
The mechanisms underlying human germ cell development and infertility remain largely unknown due to bioethical issues the shortage of experimental materials. Therefore, an effective in vitro induction system primordial germ-like cells (hPGCLCs) from pluripotent stem (hPSC) is high demand. current strategies used for generation hPGCLCs are not only costly but also difficult perform at a large scale, thereby posing barriers further research. In this study, we attempted solve these problems by...
Abstract The transition from fetal primordial germ cells (PGCs) to spermatogonia (SPG) is critical for male cell development; however, the detailed transcriptomic dynamics and regulation underlying this remain poorly understood. Here by interrogating comprehensive transcriptome atlas dataset of mouse gonadal development, we elucidated regulatory networks transition. Our single‐cell analysis revealed that PGCs SPG was characterized global hypertranscription. A total 315 highly active...
The NDUFS4 gene encodes an 18-kD subunit of mitochondria complex I, and mutations in this lead to the development a severe neurodegenerative disease called Leigh syndrome (LS) humans. To investigate phenotypes molecular mechanisms syndrome, Ndufs4 knockout (KO) mouse has been widely used as novel animal model. Because homozygotes cannot survive beyond child-bearing age, whether mitochondrial I influence early embryonic remains unknown. In our study, we attempted KO mice, which can be...
Helicase POLQ‐like (HELQ) is a DNA helicase essential for the maintenance of genome stability. A recent study identified two HELQ missense mutations in some cases infertile men. However, functions process germline specification are not well known and whether its function conserved between mouse human remains unclear. Here, we revealed that Helq knockout ( −/− ) could significantly reduce efficiency primordial germ cell‐like cell (PGCLC) induction. In addition, embryonic bodies exhibited...