A5085659911- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Renal and related cancers
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- Tissue Engineering and Regenerative Medicine
Southern Medical University
2018-2023
Abstract Mammalian male germ cell development is a stepwise cell-fate transition process; however, the full-term developmental profile of cells remains undefined. Here, by interrogating high-precision transcriptome atlas 11,598 covering 28 critical time-points, we demonstrate that from mitotic to post-mitotic primordial accompanied transcriptome-scale reconfiguration and transitional state. Notch signaling pathway essential for initiating arrest maintenance cells’ identities. Ablation HELQ...
Abstract Multiple pluripotent states have been described in mouse and human stem cells. Here, we apply single-cell RNA-seq to a newly established BMP4 induced primed naïve transition (BiPNT) system show that the reset is not direct reversal of cell fate but goes through primordial germ cell-like cells (PGCLCs) state. We first epiblast bifurcate into c-Kit + − trophoblast-like cells, among which, branch undergoes further PGCLCs intermediate capable spermatogenesis vivo. Mechanistically, DOT1L...
The mechanisms underlying human germ cell development and infertility remain largely unknown due to bioethical issues the shortage of experimental materials. Therefore, an effective in vitro induction system primordial germ-like cells (hPGCLCs) from pluripotent stem (hPSC) is high demand. current strategies used for generation hPGCLCs are not only costly but also difficult perform at a large scale, thereby posing barriers further research. In this study, we attempted solve these problems by...
Germline specification is a fundamental step for human reproduction and this biological phenomenon possesses technical challenges to study in vivo as it occurs immediately after blastocyst implantation.The establishment of vitro primordial germ cell-like cells (hPGCLCs) induction system allows sophisticated characterization (hPGCs) development.However, the underlying molecular mechanisms hPGCLC are not fully elucidated.Here, we observed particularly high expression histone demethylase KDM2B...
The spermatogonial stem cell (SSC) niche is critical for SSC maintenance and subsequent spermatogenesis. Numerous reproductive hazards impair the niche, thereby resulting in aberrant self-renewal male infertility. However, promising agents targeting impaired to promote are still limited. Here, we screen out assess effects of Lovastatin on mouse SSCs (mSSCs). Mechanistically, promotes mSSCs inhibits its inflammation apoptosis through regulation isoprenoid intermediates. Remarkably, treatment...
Abstract Single cell analysis provides clarity unattainable with bulk approaches. Here we apply single RNA-seq to a newly established BMP4 induced mouse primed naive transition (Bi-PNT) system and show that the reset is not direct reversal of fate but through developmental intermediates. We first mEpiSCs bifurcate into c-Kit+ naïve c-Kit- placenta-like cells, among which, branch undergoes further primordial germ cell-like cells (PGCLCs) intermediate capable spermatogenesis in vivo. Indeed,...