A5075104868- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Genomics and Phylogenetic Studies
- DNA and Biological Computing
- Developmental Biology and Gene Regulation
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Tissue Engineering and Regenerative Medicine
Karolinska Institutet
2022
Southern Medical University
2019-2021
The First People's Hospital of Shunde
2021
University of Chinese Academy of Sciences
2015-2019
Guangzhou Institutes of Biomedicine and Health
2014-2019
Chinese Academy of Sciences
2014-2019
Guangzhou Medical University
2017-2018
Genome Institute of Singapore
2015
Abstract Oct4, along with Sox2 and Klf4 (SK), can induce pluripotency but structurally similar factors like Oct6 cannot. To decode why Oct4 has this unique ability, we compare Oct4-binding, accessibility patterns transcriptional waves an mutant defective in the dimerization (Oct4 defSox2 ). We find that initial silencing of somatic program proceeds indistinguishably or without Oct4. mitigates mesenchymal-to-epithelial transition derails reprogramming. These effects are a consequence...
Abstract Background Exosomes play a key role in cell-to-cell communication and are integral component of the tumor microenvironment. Recent observations suggest transfer RNA through tumor-derived exosomes that can potentially translate into regulatory proteins recipient cells. Role cervical cancer-derived their transcript cargo is poorly understood. Materials methods The total from HPV-positive (SiHa HeLa) HPV-negative (C33a) cancer cell lines were extracted transcripts estimated using...
Sox2 and Pax6 are transcription factors that direct cell fate decision during neurogenesis, yet the mechanism behind how they cooperate on enhancer DNA elements regulate gene expression is unclear. By systematically interrogating interaction minimal elements, we found cooperative recognition relies combinatorial nucleotide switches precisely spaced, but cryptic composite motifs. Surprisingly, all tested Sox Pax paralogs have capacity to such elements. NMR molecular modeling reveal very few...
Highlights•Pooled screens with site-randomized reprogramming factor libraries•The phenotype of cells is used to isolate factors enhanced functions•Native are not optimized for non-native conditions•Artificially evolved accelerate reprogrammingSummaryDirected biomolecular evolution widely tailor and enhance enzymes, fluorescent proteins, antibodies but has hitherto been applied in the mammalian cells. Here, we describe a method termed directed by cell selection sequencing (DERBY-seq) identify...
The functional consequences of cancer‐associated missense mutations are unclear for the majority proteins. We have previously demonstrated that activity SOX and Pit‐Oct‐Unc (POU) family factors during pluripotency reprogramming can be switched enhanced with rationally placed point mutations. Here, we interrogated cancer mutation databases identified recurrently mutated positions at critical structural interfaces DNA‐binding domains paralogous POU transcription factors. Using conversion mouse...
Germline specification is a fundamental step for human reproduction and this biological phenomenon possesses technical challenges to study in vivo as it occurs immediately after blastocyst implantation.The establishment of vitro primordial germ cell-like cells (hPGCLCs) induction system allows sophisticated characterization (hPGCs) development.However, the underlying molecular mechanisms hPGCLC are not fully elucidated.Here, we observed particularly high expression histone demethylase KDM2B...