A5089646045- Ion Transport and Channel Regulation
- Magnesium in Health and Disease
- Hormonal Regulation and Hypertension
- Potassium and Related Disorders
- Dialysis and Renal Disease Management
- Blood Pressure and Hypertension Studies
- Pancreatitis Pathology and Treatment
- ATP Synthase and ATPases Research
- Electrolyte and hormonal disorders
- Central Venous Catheters and Hemodialysis
- Aldose Reductase and Taurine
- Ion channel regulation and function
- Genetic Syndromes and Imprinting
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Parathyroid Disorders and Treatments
- Inflammasome and immune disorders
- Diet, Metabolism, and Disease
- Eicosanoids and Hypertension Pharmacology
- Renin-Angiotensin System Studies
- Climate Change and Health Impacts
- Cardiac Arrhythmias and Treatments
- Adrenal Hormones and Disorders
- Folate and B Vitamins Research
- Sodium Intake and Health
Hiroshima University Hospital
2017-2025
Oregon Health & Science University
2020-2024
Hiroshima University
2018-2024
University of Portland
2021-2023
Hiroshima Prefectural Hospital
2014
Background: MR (mineralocorticoid receptor) antagonists are recommended for patients with resistant hypertension even when circulating aldosterone levels not high. Although activates to increase epithelial sodium channel (ENaC) activity, glucocorticoids also activate but metabolized by 11βHSD2 (11β-hydroxysteroid dehydrogenase type 2). is expressed at increasing from distal convoluted tubule (DCT) through collecting duct. Here, we hypothesized that maintains ENaC activity in the DCT2 and...
Peritoneal inflammation and fibrosis remain major challenges to the long-term maintenance of peritoneal dialysis. Pemafibrate, a selective peroxisome proliferator-activated receptor α (PPARα) modulator, has been implicated in management fibrosis-related disorders. We investigated whether pemafibrate ameliorates explored underlying mechanisms mice with methylglyoxal (MGO)-induced (MGO mice). MGO exhibited increased expression mesenchymal markers, transforming growth factor-β1 (TGF-β1),...
ABSTRACT Background Haemodiafiltration (HDF) therapy improves the prognosis by reducing inflammation and oxidative stress improving endothelial function. These factors contribute to vascular access (VA) stenosis, one of most common complications in patients on haemodialysis (HD) or HDF. This study aimed assess efficacy HDF VA patency. Methods multicentre, prospective, observational with post hoc analysis included 612 among 643 who underwent procedures started dialysis between April 2012...
ABSTRACT Peritoneal fibrosis poses a significant challenge to the long‐term efficacy of peritoneal dialysis (PD), with emerging evidence highlighting role cellular senescence in its pathogenesis. p16 INK4a is cell cycle regulator that has been implicated senescence. Mixed‐lineage leukemia 1 (MLL1) forms complex WD‐40 repeat protein 5 (WDR5) and exhibits histone H3K4 methyltransferase activity. We have previously shown inhibition MLL1/WDR5 reduces expression attenuates renal after injury...
Significance Statement High-resolution single-nucleus RNA-sequencing data indicate a clear separation between primary sites of calcium and magnesium handling within distal convoluted tubule (DCT). Both DCT1 DCT2 express Slc12a3, but these subsegments serve distinctive functions, with more abundant magnesium-handling genes along calcium-handling DCT2. The also provide insight into the plasticity nephron-collecting duct junction, formed from cells separate embryonic origins. By...
Significance Statement Familial hyperkalemic hypertension (FHHt) results from inappropriate activation of the Na + Cl – cotransporter (NCC). Causative mutations have been identified in gene encoding Cullin3 (CUL3). with substrate binding adaptor Kelch-like 3 (KLHL3) forms an E3 ubiquitin ligase that mediates degradation NCC regulatory kinases. The mechanism by which mutant CUL3 causes FHHt is unclear, but lower abundance and KLHL3 proteins loss to regulator JAB1 implicated. Using several...
The renal cation cotransporters NCC and NKCC2 are activated following phosphorylation mediated by the WNK4-SPAK/OSR1 pathway. While disruption of this pathway strongly affects activity, effects on activity unclear since commonly used phospho-NKCC2 antibody was recently reported to cross-react with phospho-NCC in mice C57BL/6 background. Using a new specific for C57BL/6, we show that inhibition or activation only mildly phosphorylation.
Klotho is an antiaging protein reported to suppress transforming growth factor-β1 (TGF-β1) signaling. Aging kidneys are characterized by interstitial fibrosis, accumulation of cell cycle-arrested cells, and increased levels oxidative stress. TGF-β1 signaling involved in these processes. In this study, we investigated whether klotho overexpression improves features the aging mice examined inhibitory effect on molecules related TGF-β1. transgenic (KLTG) wild-type (WT) were used, 8-wk-old...
We previously reported that among the 37 RING finger protein (RNF) family members, RNF183 mRNA is specifically expressed in kidney under normal conditions. However, mechanism supporting its kidney-specific expression pattern remains unclear. In this study, we elucidated of transcriptional activation murine Rnf183 inner-medullary collecting duct cells. Experiments with anti-RNF183 antibody revealed predominantly renal medulla. Among RNF was increased hypertonic conditions, a hallmark...
Abstract Background Primary coenzyme Q10 (CoQ10) deficiency of genetic origin is one a few treatable focal segmental glomerulosclerosis (FSGS). Renal morphologic evidence for COQ8B mutation and CoQ10 deficiencies other gene mutations assessed using electron microscopy with marked increase abnormal-shaped mitochondria in podocytes. However, light microscopic features than FSGS have not been reported. Case presentation A 30-year-old woman was admitted to our hospital because proteinuria found...
Abstract Background This study aims to compare patency rates of the 0- and 30-s (sec) balloon dilation time in hemodialysis (HD) patients with restenosis after percutaneous transluminal angioplasty (PTA). Methods The who underwent PTA within 6 months for failed arteriovenous fistula at forearm were randomly assigned 0-s or group. Effect on 3- 6-month was examined. Results Fifty enrolled this study. 3-month rate group better than that ( P = 0.0050), while did not show a significant difference...
Peritoneal dialysis (PD)-associated peritonitis is a common complication of PD. Enteric defined as arising from an intestinal or intra-abdominal organ source. The delay in the diagnosis treatment enteric has been reported to increase mortality. Therefore, early consideration peritonitis, particularly cases culture-negative imperative. A 67-year-old Japanese man who had undergoing PD for 3 years, was admitted our hospital with PD-associated peritonitis. month previously, he experienced...
Mutations in the ubiquitin ligase scaffold protein cullin 3 (CUL3) cause disease familial hyperkalemic hypertension (FHHt). We recently reported that kidney, aberrant mutant CUL3 (CUL3-Δ9) activity lowers abundance of CUL3-Δ9 and Kelch-like 3, substrate adaptor for with-no-lysine kinase 4 (WNK4) this is mechanistically important. However, whether exerts additional effects on other targets may alter renal function unclear. Here, we sought to determine 1) expression can rescue phenotype...
We identified 37 ubiquitin ligases containing RING-finger and transmembrane domains. Of these, we found that RNF183 is abundantly expressed in the kidney. predominantly localizes to endoplasmic reticulum (ER), Golgi, lysosome. Sec16A, which involved coat protein complex II vesicle formation, as an RNF183-interacting protein. colocalized with Sec16A interacted through central conserved domain (CCD) of Sec16A. Although not a substrate for RNF183, was more rapidly degraded by ER-associated...
Abstract Background The distal convoluted tubule (DCT) comprises two subsegments, DCT1 and DCT2, with different functional molecular characteristics. distinction between these segments, however, has been controversial. Methods To understand the heterogeneity within DCT population better clarity, we enriched for nuclei by using a mouse line combining “Isolation of Nuclei TAgged in specific Cell Types” NCC (sodium chloride cotransporter)-driven inducible Cre recombinase. We sorted...
The number of new dialysis patients has been increasing worldwide, particularly among elderly individuals. However, information on seasonal variation in hemodialysis initiation recent decades is lacking, and the distribution patients' conditions immediately prior to starting remains unclear. Having this could help developing a modifiable approach improving pre-dialysis care. We retrospectively investigated records 297 who initiated at Hiroshima Prefectural Hospital from January 1st, 2009...
Abstract Background: Marked activation of the renin-angiotensin-aldosterone system (RAAS) plays an important role in hypertensive emergencies, particular malignant hypertension, by worsening hypertension and renal function. The rates readmission for severe cardiovascular disease such emergencies are high, suggesting that suppression RAAS may be not adequate during acute phase some cases. Although treatment with a combination inhibitors decreases blood pressure (BP) to greater extent than...