A5012725289- Endoplasmic Reticulum Stress and Disease
- Barrier Structure and Function Studies
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Gut microbiota and health
- Ubiquitin and proteasome pathways
- Aldose Reductase and Taurine
- RNA Research and Splicing
- Lysosomal Storage Disorders Research
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- Oral microbiology and periodontitis research
- Pancreatic function and diabetes
- Caveolin-1 and cellular processes
- Adipose Tissue and Metabolism
- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- Connexins and lens biology
- Hedgehog Signaling Pathway Studies
- Nerve injury and regeneration
- Epigenetics and DNA Methylation
- Clostridium difficile and Clostridium perfringens research
- Phytochemistry and biological activity of medicinal plants
- Hereditary Neurological Disorders
- Nuclear Structure and Function
Nanyang Technological University
2025
Hiroshima University
2015-2023
Osaka University
2009-2015
Kobe University
2011
Soka University
2011
Abstract Endoplasmic reticulum (ER)-associated degradation (ERAD) is a mechanism by which unfolded proteins that accumulate in the ER are transported to cytosol for ubiquitin–proteasome-mediated degradation. Ubiquitin ligases (E3s) group of enzymes responsible substrate selectivity and ubiquitin chain formation. The purpose this study was identify novel E3s involved ERAD. Thirty-seven candidate genes were selected searches with RING-finger motifs transmembrane regions, major features ERAD...
CREB/ATF transcription factor OASIS/CREB3L1 is upregulated in long-term-cultured astrocytes undergoing cell-cycle arrest due to loss of DNA integrity by repeated replication. However, the roles OASIS cell cycle remain unexplored. We find that arrests at G2/M phase after damage via direct induction p21. Cell-cycle dominant and osteoblasts, but not fibroblasts, which are dependent on p53. In a brain injury model, Oasis−/− reactive surrounding lesion core show sustained growth inhibition...
Unfolded protein response (UPR) has roles not only in resolving the accumulation of unfolded proteins owing to endoplasmic reticulum (ER) stress, but also regulation cellular physiological functions. ER stress transducers providing branches UPR signaling are known localize distal dendritic neurons. These reports suggest that local activation may produce integrated outputs for distant communication, and allow events highly polarized Here, we demonstrated synaptic activity- brain-derived...
Ubiquitylation is a post-translational modification originally identified as the first step in protein degradation by ubiquitin–proteasome system. also known to regulate many cellular processes without degrading ubiquitylated proteins. Substrate proteins are specifically recognized and ubiquitin ligases. It necessary identify substrates for each ligase understand physiological pathological roles of ubiquitylation. Recently, promiscuous mutant biotin derived from Escherichia coli, BioID, its...
Abstract The unfolded protein response (UPR) not only resolves endoplasmic reticulum (ER) stress, but also regulates cellular physiological functions. In this study, we first linked the UPR to roles of brown adipose tissue (BAT). BAT is one tissues that control energy homeostasis in body. Brown adipocytes are able dissipate form heat owing their mitochondrial protein, uncoupling 1 (UCP1). We found branches, IRE1α-XBP1 pathway, was activated during transcriptional induction Ucp1 . Inhibiting...
Abstract OASIS/CREB3L1, an endoplasmic reticulum (ER)-resident transcription factor, plays important roles in osteoblast differentiation. In this study, we identified new crosstalk between OASIS and the hypoxia signaling pathway, which regulates vascularization during bone development. RT-PCR real-time PCR analyses revealed significant decreases expression levels of hypoxia-inducible factor-1α (HIF-1α) target genes such as vascular endothelial growth factor A (VEGFA) OASIS-deficient ( Oasis...
Abstract The nuclear envelope (NE) safeguards the genome and is pivotal for regulating activity as structural scaffold of higher-order chromatin organization. NE had been thought stable during interphase cell cycle. However, recent studies have revealed that can be damaged by various stresses such mechanical stress cellular senescence. These types are called stress. It has proposed closely related to dysfunctions instability death. Here, we found an endoplasmic reticulum (ER)-resident...
Tumor metastasis of epithelium-derived tumors is the major cause death from malignant tumors. Overexpression claudin observed frequently in However, claudin-targeting antimetastasis therapy has never been investigated. We previously prepared a claudin-4-targeting antitumor molecule that consisted C-terminal fragment Clostridium perfringens enterotoxin (C-CPE) fused to protein synthesis inhibitory factor (PSIF) derived Pseudomonas exotoxin. In present study, we investigated whether CPE...
Luman is a type II transmembrane transcription factor belonging to the OASIS family that localizes endoplasmic reticulum (ER) membrane under normal conditions. In response ER stress, members are subjected regulated intramembrane proteolysis (RIP), following which cleaved N-terminal fragments translocate nucleus. this study, we show treatment of bone marrow macrophages (BMMs) with cytokines, M-CSF and RANKL, causes time-dependent increase in expression undergoes RIP becomes activated during...
Adult mammalian peripheral neurons have an intrinsic regrowth capacity in response to axonal injury. The induction of calcium ion (Ca2+) oscillations at injured site is critical for the regulation regenerative responses. In polarized neurons, distal segments contain a well-developed endoplasmic reticulum (ER) network that responsible Ca2+ homeostasis. Although these characteristics implicate relevance among injury-induced dynamics, ER-derived signaling, and responses propagated along axons,...
Abstract Mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, is a devastating progressive disease caused by mutations in the iduronate 2-sulfatase ( IDS ) gene. one of sulfatase enzymes required for lysosomal degradation glycosaminoglycans. Mutant proteins linked to diseases are often prone misfolding. These misfolded accumulate endoplasmic reticulum (ER) and degraded ubiquitin–proteasome pathway (ER-associated (ERAD)). The decreased enzyme activities mutants may be due...
Abstract Androgen-Induced bZIP (AIbZIP) is structurally a transmembrane transcription factor belonging to the CREB/ATF family. This molecule highly expressed in androgen-sensitive prostate cancer cells and transcriptionally upregulated by androgen treatment. Here, we investigated molecular mechanism of androgen-dependent expression AIbZIP its physiological function cells. Our data showed that SAM pointed domain-containing ETS (SPDEF), which treatment, directly activates . Knockdown caused...
Claudins are key functional and structural components of tight junctions (TJs) in epithelial cell sheets. The C-terminal fragment Clostridium perfringens enterotoxin (C-CPE) binds to claudin-4 reversibly modulates intestinal TJ seals, thereby enhancing paracellular transport solutes. However, the use C-CPE as an absorption enhancer is limited by molecule's immunogenicity manufacturing cost. Here, we developed a high-throughput screening system based on Time-Resolved Fluorescence Resonance...
We previously reported that among the 37 RING finger protein (RNF) family members, RNF183 mRNA is specifically expressed in kidney under normal conditions. However, mechanism supporting its kidney-specific expression pattern remains unclear. In this study, we elucidated of transcriptional activation murine Rnf183 inner-medullary collecting duct cells. Experiments with anti-RNF183 antibody revealed predominantly renal medulla. Among RNF was increased hypertonic conditions, a hallmark...
Abstract RNF183 is a ubiquitin ligase containing RING-finger and transmembrane domains, its expression levels are increased in patients with inflammatory bowel disease (IBD), including Crohn’s ulcerative colitis, 2,4,6-trinitrobenzene sulfonic acid-induced colitis mice. Here, we further demonstrate that was induced to greater degree the dextran sulfate sodium (DSS)-treated IBD model at very early stage than were cytokines. In addition, fluorescence-activated cell sorting polymerase chain...