A5026660217- Ion Transport and Channel Regulation
- Renal and related cancers
- Hormonal Regulation and Hypertension
- Electrolyte and hormonal disorders
- Birth, Development, and Health
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Magnesium in Health and Disease
- Neuroendocrine regulation and behavior
- Pancreatic function and diabetes
- ATP Synthase and ATPases Research
- Neonatal Respiratory Health Research
- Ion channel regulation and function
- Sirtuins and Resveratrol in Medicine
- Antimicrobial Peptides and Activities
- Chronic Kidney Disease and Diabetes
- Renin-Angiotensin System Studies
- RNA Interference and Gene Delivery
- Natural product bioactivities and synthesis
- Cancer, Hypoxia, and Metabolism
- PARP inhibition in cancer therapy
- Genetic and Kidney Cyst Diseases
- Renal Diseases and Glomerulopathies
- Essential Oils and Antimicrobial Activity
Johns Hopkins Medicine
2020-2025
Johns Hopkins University
2020-2025
University of Maryland, Baltimore
2019-2024
National Heart Lung and Blood Institute
2015-2021
National Institutes of Health
2015-2021
Kyungpook National University
2008-2020
Technical University of Munich
2020
University of Maryland, Baltimore County
2018
Jungheinrich (Germany)
2015
Inha University Hospital
2010-2013
The distal convoluted tubule (DCT) plays an indispensable role in magnesium (Mg2+) reabsorption the kidney. Yet, extrusion mechanism of Mg2+ has not been identified. solute carrier 41A3 (SLC41A3) suggested to be involved extrusion, but this never conclusively demonstrated. Using available RNA-sequencing data and real-time quantitative PCR, expression two alternative Slc41a3 transcripts, encoding isoform (Iso) 1 or 2, were assessed kidney isolated DCT tubules. HEK293 HAP1 cells transfected...
Aquaporin (AQP) is a family of transmembrane proteins for water transport. Recent studies revealed that AQPs are likely to play role in tumor progression and invasion. We aimed examine the potential AQP5 human breast cancer cells. Expression mRNA protein was seen cell line (both MCF7 MDA-MB-231) by RT-PCR immunoblotting analysis. Immunoperoxidase labeling observed at ductal epithelial cells tissues. In benign tumor, mainly apical domains contrast, invasive carcinoma, prominent associated...
Significance Maintenance of homeostasis is dependent on intercellular communication via secreted hormones that bind G protein-coupled receptors. Many these receptors activate an enzyme called protein kinase A (PKA) modifies cell function by covalently attaching phosphate groups to proteins. To comprehensively identify PKA substrates, we used genome editing (CRISPR-Cas9) delete from kidney epithelial cells followed large-scale mass spectrometry measure phosphorylation changes throughout the...
Aquaporin-5 (AQP5) plays a role in breast cancer cell migration. This study aimed to identify AQP5-targeting miRNAs and examine their effects on migration through exosome-mediated delivery. Bioinformatic analyses identified miR-1226-3p, miR-19a-3p, miR-19b-3p as putative regulators of AQP5 mRNA. Immunoblotting revealed decrease protein abundance when each these was transfected into human MDA-MB-231 cells. Quantitative real-time PCR demonstrated the reduction mRNA expression by transfection...
Abstract Loss of a kidney results in compensatory growth the remaining kidney, phenomenon considerable clinical importance. However, mechanisms involved are largely unknown. Here, we use multi-omic approach unilateral nephrectomy model male mice to identify signaling processes associated with renal hypertrophy, demonstrating that lipid-activated transcription factor peroxisome proliferator-activated receptor alpha (PPARα) is an important determinant proximal tubule cell size and likely...
The E3 ubiquitin (Ub)-protein ligases (E3s) play a role as regulators of protein trafficking and degradation. We aimed to integrate the profile E3s in rat kidney examine changes abundance selected response 1-deamino-8-D-arginine vasopressin (dDAVP) stimulation/withdrawal. Sprague-Dawley rats were infused with vehicle (n = 13), dDAVP for 5 days or was withdrawn periods (15 min, 30 1, 3, 6, 12, 24 h) after 5-day infusion 46). Total RNA isolated from inner medulla (IM) transcriptome analysis....
Mature microRNA (miRNA) acts as an important posttranscriptional regulator. We aimed to profile vasopressin-responsive miRNAs in kidney inner medullary collecting duct cells and identify aquaporin-2 (AQP2)-targeting miRNAs. Microarray chip assay was carried out tubule suspensions from rat kidneys the absence or presence of desmopressin (dDAVP) stimulation (10(-9) M, 2 h). The results demonstrated 19 miRNAs, including both precursor mature potential candidates that showed significant changes...
Mineralocorticoids trigger a profibrotic process in the kidney. In mouse cortical collecting duct cells, present study addressed two main questions: 1) what are microRNAs (miRNAs) and their target genes that changed by aldosterone? 2) do miRNAs, response to aldosterone, regulate regarding signaling pathways related fibrosis? A microarray chip assay was done cells absence or presence of aldosterone treatment (10-6 M; 3 days). The candidate miRNAs were identified criteria >30% fold change...
Significance Statement High-resolution single-nucleus RNA-sequencing data indicate a clear separation between primary sites of calcium and magnesium handling within distal convoluted tubule (DCT). Both DCT1 DCT2 express Slc12a3, but these subsegments serve distinctive functions, with more abundant magnesium-handling genes along calcium-handling DCT2. The also provide insight into the plasticity nephron-collecting duct junction, formed from cells separate embryonic origins. By...
AS160, a novel Akt substrate of 160 kDa, contains Rab GTPase-activating protein (GAP) domain. The present study examined the role and AS160 in aquaporin-2 (AQP2) trafficking. main strategy was to examine changes AQP2 translocation response small interfering RNA (siRNA)-mediated knockdown mouse cortical collecting duct cells (M-1 mpkCCDc14 cells). Short-term dDAVP treatment M-1 stimulated phosphorylation (S473) which also seen cells. Conversely, phosphoinositide 3-kinase (PI3K) inhibitor LY...
Compared with the blind technique, ultrasound-guided stellate ganglion block (SGB) reduces amount of local anesthetic needed for a successful block. The purpose this study is to determine minimal, optimal volume required SGB and reduce its adverse effects. Thirty-five patients postherpetic neuralgia complex regional pain syndrome upper extremity facial area were selected. For by subfacial method, each patient was injected 0.5% mepivacaine mixed contrast media in 2 mL, 3 4 mL doses at 2-week...
Background Renal water excretion is controlled by vasopressin, in part through regulation of the transcription aquaporin-2 gene ( Aqp2 ). Methods To identify enhancer regions likely to be involved and other principal cell–specific genes, we used several next generation DNA-sequencing techniques a well characterized cultured cell model collecting duct cells (mpkCCD). locate enhancers, performed assay for transposase-accessible chromatin using sequencing (ATAC-Seq) accessible DNA integrated...
Abstract T cells are important in the pathogenesis of acute kidney injury (AKI), and TCR + CD4 - CD8 (double negative-DN) that have regulatory properties. However, there is limited information on DN compared to traditional cells. To elucidate molecular signature spatial dynamics during AKI, we performed single-cell RNA sequencing (scRNA-seq) sorted murine DN, , combined with transcriptomic profiling normal post AKI mouse kidneys. scRNA-seq revealed distinct transcriptional profiles for...