A5036410920- Renal Transplantation Outcomes and Treatments
- Acute Kidney Injury Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Renal and related cancers
- Cancer Immunotherapy and Biomarkers
- Clinical Nutrition and Gastroenterology
- Chronic Kidney Disease and Diabetes
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- Metabolism and Genetic Disorders
- CRISPR and Genetic Engineering
- Polyomavirus and related diseases
- Electrolyte and hormonal disorders
- Sodium Intake and Health
- Metabolism, Diabetes, and Cancer
- Amino Acid Enzymes and Metabolism
- Hematopoietic Stem Cell Transplantation
- Cancer, Hypoxia, and Metabolism
- Cytomegalovirus and herpesvirus research
- Blood Pressure and Hypertension Studies
- Muscle and Compartmental Disorders
Johns Hopkins Medicine
2019-2024
Johns Hopkins University
2019-2024
Universitätsklinikum Erlangen
2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2017-2024
University of Baltimore
2023
Johns Hopkins Hospital
2020
Significance Statement T cells mediate pathogenic and reparative processes during AKI, but the exact mechanisms regulating kidney cell functions are unclear. This study identified upregulation of novel immune checkpoint molecule, TIGIT, on mouse human after AKI. TIGIT-expressing produced proinflammatory cytokines had effector (EM) central memory (CM) phenotypes. TIGIT-deficient mice protection from both ischemic nephrotoxic Single-cell RNA sequencing led to discovery possible downstream...
CD4+ T cells mediate the pathogenesis of ischemic and nephrotoxic acute kidney injury (AKI). However, underlying mechanisms cell-mediated are largely unknown. We therefore conducted unbiased RNA-sequencing to discover novel mechanistic pathways after ischemia compared with normal mouse kidney. Unexpectedly, lipocalin-2 (Lcn2) gene, which encodes neutrophil gelatinase-associated lipocalin (NGAL) had highest fold increase (∼60). The NGAL in during AKI was confirmed at mRNA level quantitative...
T cells play an important role in acute kidney injury (AKI). Metabolic programming of regulates their function, is a rapidly emerging field, and unknown AKI. We induced ischemic AKI C57BL/6J mice collected kidneys spleens at multiple time points. were isolated analyzed by immune-metabolic assay. Unbiased machine learning analyses identified distinct cell subset with reduced voltage-dependent anion channel 1 mTOR expression post-AKI kidneys. Ischemic showed higher trimethylation histone H3...
T cells play pathophysiologic roles in kidney ischemia-reperfusion injury (IRI), and the nuclear factor erythroid 2-related 2/kelch-like ECH-associated protein 1 (Nrf2/Keap1) pathway regulates cell responses. We hypothesized that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9)-mediated
Abstract T cells are important in the pathogenesis of acute kidney injury (AKI), and TCR + CD4 - CD8 (double negative-DN) that have regulatory properties. However, there is limited information on DN compared to traditional cells. To elucidate molecular signature spatial dynamics during AKI, we performed single-cell RNA sequencing (scRNA-seq) sorted murine DN, , combined with transcriptomic profiling normal post AKI mouse kidneys. scRNA-seq revealed distinct transcriptional profiles for...
T cells mediate organ injury and repair. A proportion of unconventional kidney called double-negative (DN) (TCR
Acute kidney injury (AKI) due to cisplatin is a significant problem that limits its use as an effective chemotherapeutic agent. T cell receptor + CD4 − CD8 double negative (DN) cells constitute the major population in human and mouse kidney, express programmed death protein (PD)-1, protect from ischemic AKI. However, pathophysiological roles of DN cisplatin-induced AKI unknown. In this study, wild-type mice were treated with (30 mg/kg) or vehicle, effects on numbers function measured. vitro...
Abstract Background Salt intake in CKD patients can affect cardiovascular risk and kidney disease progression. Twenty-four hour (24h) urine collections are often used to investigate salt metabolism but cumbersome perform. We assessed urinary sodium (U-Na) concentration spot samples investigated the correlation with 24h U-Na excretion under nephrological care. Further, we studied role of stage diuretics evaluated performance commonly formulas for prediction from samples. Methods One hundred...
Abstract Background: We unexpectedly found upregulated expression of T cell immunoreceptor with Ig and ITIM domains (TIGIT) on kidney CD4 cells after ischemia reperfusion (IR) using RNA-Seq. However, TIGIT role in during acute injury (AKI) is poorly understood. Methods: Effect murine was assessed at baseline IR by flow cytometry single RNA sequencing (scRNA-Seq). B6 wild type (WT) knockout (KO) mice were subjected to cisplatin-induced AKI. AKI patients the Kidney Precision Medicine Project...
Key Points Experimental studies often fail to translate clinical practice. Humanized mouse models are an important tool close this gap. We immunophenotyped the kidneys of NOG (EXL) and NSG strains engrafted with human CD34 + hematopoietic stem cells or PBMCs compared immune cell composition normal kidney. Human engraftment results in steady renal populations kidney key similarities Successful translation experimental data diseases is limited because biological differences imperfect disease...
INTRODUCTION AND AIMS: Mortality in patients with stage 4 and 5 chronic kidney disease is higher than the general population.Overhydration associated mortality hemodialysis.The relative hydration status (overhydration/extracellular water) measured by bioimpedance 15% has shown an increased risk hemodialysis patients, but this association not been described advanced disease.Our objective was to evaluate effect of overhydration on nondialysis disease.METHODS: We performed a prospective study...
Sadasivam, Mohanraj; Noel, Sanjeev; fathabad, Somayeh Gharaie; Kurzhagen, Johanna T.; Hamad, Abdel; Rabb, Hamid Author Information
Kurzhagen, Johanna T.; Noel, Sanjeev; fathabad, Somayeh Gharaie; Sadasivam, Mohanraj; Lee, Sul A; Gong, Jing; Arend, Lois J.; Hamad, Abdel; Rabb, Hamid Author Information
Noel, Sanjeev; Lee, Kyungho; Gharaie, Sepideh; Kurzhagen, Johanna T.; Arend, Lois J.; Cahan, Patrick; Rabb, Hamid Author Information
Lee, Kyungho; Gharaie, Sepideh; Kurzhagen, Johanna T.; Arend, Lois J.; Noel, Sanjeev; Rabb, Hamid Author Information
Lee, Kyungho; Thompson, Elizabeth A.; Gharaie, Sepideh; Patel, Chirag H.; Kurzhagen, Johanna T.; Newman-Rivera, Andrea M.; Pierorazio, Phillip Arend, Lois J.; Noel, Sanjeev; Slusher, Barbara S.; Rabb, Hamid Author Information