A5017457434- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Head and Neck Surgical Oncology
- Gold and Silver Nanoparticles Synthesis and Applications
- Tracheal and airway disorders
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Image Analysis Techniques
- Electrohydrodynamics and Fluid Dynamics
- Nanoplatforms for cancer theranostics
- nanoparticles nucleation surface interactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Biosensing Techniques and Applications
- Algal biology and biofuel production
- Airway Management and Intubation Techniques
- Nanopore and Nanochannel Transport Studies
ETH Zurich
2020-2024
Bioengineering (Switzerland)
2020-2024
IBM Research - Zurich
2020-2022
Apex Heart Institute
2010
Abstract Liquid–liquid phase separation of polymer and protein solutions is central in many areas biology material sciences. Here, an experimental theoretical framework provided to investigate the thermodynamics kinetics liquid–liquid volumes comparable cells. The strategy leverages droplet microfluidics accurately measure volume dense generated by confined micro‐sized compartments. It shown that measurement fraction at different temperatures allows evaluation binodal lines determine...
Due to their ability selectively target pathogen-specific nucleic acids, CRISPR-Cas systems are increasingly being employed as diagnostic tools. "One-pot" assays that combine acid amplification and (NAAT–CRISPR-Cas) in a single step have emerged one of the most popular biosensing formats. However, operational simplicity comes at cost, with one-pot typically less sensitive than corresponding two-step NAAT–CRISPR-Cas often failing detect targets low concentrations. It is thought these...
Due to their ability selectively target pathogen-specific nucleic acids, CRISPR-Cas systems are increasingly being employed as diagnostic tools. “One pot” assays that combine acid amplification and (NAAT–CRISPR-Cas) in a single step have emerged one of the most popular biosensing formats. However, operational simplicity comes at cost, with one-pot typically less sensitive than corresponding two-step NAAT–CRISPR-Cas often failing detect targets low concentrations. It is thought these...
Due to their ability selectively target pathogen-specific nucleic acids, CRISPR-Cas systems are increasingly being employed as diagnostic tools. “One pot” assays that combine acid amplification and (NAAT–CRISPR-Cas) in a single step have emerged one of the most popular biosensing formats. However, operational simplicity comes at cost, with one-pot typically less sensitive than corresponding two-step NAAT–CRISPR-Cas often failing detect targets low concentrations. It is thought these...
We present a method for monitoring spatially localized antigen-antibody binding events on physiologically relevant substrates (cell and tissue sections) using fluorescence lifetime imaging. Specifically, we use the difference between decay times of fluorescently tagged antibodies in free solution bound state to track fraction over time hence deduce kinetics. make microfluidic probe format minimize mass transport effects localize analysis specific regions interest biological substrates. This...
We present a novel method for the local retrieval of surface bound species and their rapid in-line separation using an open space microfluidic device. Separation can be performed in less than 30 s difference diffusivities within parallel flows. As proof-of-principle, we report efficient filtration polystyrene beads from small molecules red blood cells dimethyl sulfoxide antigen typing.
Abstract Camera‐based super‐resolution approaches surpass the diffraction limit of conventional optical microscopy by relying on stochastic activation and precise localization fluorescent molecules. However, traditional probes such as organic dyes quantum dots present challenges photobleaching blinking variability, which their application in imaging, particularly non‐liquid environments. Herein, study demonstrates potential gold nanoparticles a promising alternative for localization‐based...
Cells can dynamically organize reactions through the formation of biomolecular condensates. These viscoelastic networks exhibit complex material properties and mesoscale architectures, including ability to form multi-phase assemblies. Understanding molecular mechanisms underlying compartmentalized condensates has implications not only in biology but also development advanced materials. In this study, we demonstrate that aging heterotypic protein-RNA lead double-emulsion structures. By...