A5018064873- Advanced Polymer Synthesis and Characterization
- Nanoparticle-Based Drug Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- RNA Research and Splicing
- biodegradable polymer synthesis and properties
- Protein purification and stability
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Supramolecular Self-Assembly in Materials
- Surface Modification and Superhydrophobicity
- Alzheimer's disease research and treatments
- Microfluidic and Bio-sensing Technologies
- Virus-based gene therapy research
- Pickering emulsions and particle stabilization
- Prion Diseases and Protein Misfolding
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Evolution and Genetic Dynamics
- Microtubule and mitosis dynamics
- Algal biology and biofuel production
- Viral Infectious Diseases and Gene Expression in Insects
ETH Zurich
2018-2024
Novo Nordisk (Denmark)
2023-2024
Bioengineering (Switzerland)
2018-2021
Massachusetts Institute of Technology
2018-2021
Politecnico di Milano
2016-2021
École Polytechnique Fédérale de Lausanne
2018-2020
Mylan (Switzerland)
2017-2018
Curculio Institute
2017
MSSCORPS (Taiwan)
2017
Mylan (South Africa)
2017
Abstract Cellular, inter‐organismal and cross kingdom communication via extracellular vesicles (EVs) is intensively studied in basic science with high expectation for a large variety of bio‐technological applications. EVs intrinsically possess many attributes drug delivery vehicle. Beyond the implications cell biology, academic industrial interests have increased last few years. Microalgae constitute sustainable renewable sources bioactive compounds range sectoral applications, including...
The maturation of liquid-like protein condensates into amyloid fibrils has been associated with several neurodegenerative diseases. However, the molecular mechanisms underlying this liquid-to-solid transition have remained largely unclear. Here we analyse formation mediated by condensation low-complexity domain hnRNPA1, a involved in amyotrophic lateral sclerosis. We show that phase separation and fibrillization are connected but distinct processes modulated different regions sequence. By...
Abstract The utility of messenger RNA (mRNA) as a therapy is gaining broad interest due to its potential for addressing wide range diseases, while effective delivery mRNA molecules various tissues still poses challenge. This study reports on the design and characterization new ionizable amino‐polyesters (APEs), synthesized via ring opening polymerization (ROP) lactones with tertiary amino‐alcohols that enable tissue cell type selective mRNA. With diverse library APEs formulated into lipid...
Biophysical and biochemical characterisation of microalgae-derived extracellular vesicles.
Abstract Several RNA binding proteins involved in membraneless organelles can form pathological amyloids associated with neurodegenerative diseases, but the mechanisms of how this aggregation is modulated remain elusive. Here we investigate heterotypic protein–RNA interactions modulate condensation and liquid to amyloid transition hnRNPA1A, a protein amyothropic lateral sclerosis. In absence RNA, formation condensates promotes hnRNPA1A fibrils are localized at interface condensates. Addition...
Abstract Organisms use organic molecules called osmolytes to adapt environmental conditions. In vitro studies indicate that thermally stabilize proteins, but mechanisms are controversial, and systematic within the cellular milieu lacking. We analyzed Escherichia coli human protein thermal stabilization by in situ across proteome. Using structural proteomics, we probed osmolyte effects on stability, structure aggregation, revealing common also osmolyte- protein-specific effects. All tested...
Abstract The production of new proteins with messenger RNA (mRNA) has gained a broad interest due to its potential for addressing wide range diseases. Here, the design and characterization novel ionizable poly(β‐amino ester)‐ co ‐poly(caprolactone) terpolymers, synthesized via combination ring opening polymerization Michael step‐growth polymerization, are reported. versatility this method is demonstrated by varying number caprolactone units attached each ester) (PBAE) terpolymer. ability...
The available strategies to combine CRPs and ROP in the synthesis of highly engineered polymer nanoparticles are here critically discussed.
Abstract Cells can form membraneless organelles by liquid–liquid phase separation. As these are highly dynamic, it is crucial to understand the kinetics of transitions. Here, we use droplet‐based microfluidics mix reagents chaotic advection and observe nucleation, growth, coarsening in volumes comparable cells (pL) on timescales seconds. We apply this platform analyze dynamics synthetic formed DEAD‐box ATPase Dhh1 RNA, which associated with formation processing bodies yeast. show that...
Biomolecular condensates sequester an aggregation-prone peptide and prevent its aggregation, showing that heterotypic interactions within the can formation of amyloid fibrils, despite local increase in concentration.
We report tunable biodegradable zwitterionic nanoparticles with UCST behavior under physiological conditions that can be used in controlled delivery strategies.
Abstract Liquid–liquid phase separation of polymer and protein solutions is central in many areas biology material sciences. Here, an experimental theoretical framework provided to investigate the thermodynamics kinetics liquid–liquid volumes comparable cells. The strategy leverages droplet microfluidics accurately measure volume dense generated by confined micro‐sized compartments. It shown that measurement fraction at different temperatures allows evaluation binodal lines determine...
We develop and present a one-pot sandwich immunoassay (termed oneSTEP) to detect target biomolecules in complex biological fluids based on programmable zwitterionic polymer coacervates. design these coacervates selectively recruit analytes with ultralow nonspecific adsorption. show that dynamic compartmentalization combined local enrichment delivers rapid wash-free high specificity signal-to-noise ratio. The fluorescence-based readout is performed using standard microscopy methods flow...
Abstract The front cover artwork is provided by the Arosio Lab at ETH Zurich. image shows liquid phase separated droplets that are based on biologically inspired intrinsically disordered proteins and act as microreactors for enhanced enzymatic reactions. Read full text of Article 10.1002/syst.202000001 .
Increasing evidence indicates that cells can regulate biochemical functions in time and space by generating membraneless compartments with well-defined mesoscopic properties. One important mechanism underlying this control is simple coacervation driven associative disordered proteins encode multivalent interactions. Inspired these observations, programmable droplets based on of responsive synthetic polymers mimic the "stickers-and-spacers" architecture biological are developed. Zwitterionic...
Abstract The maturation of liquid-like protein condensates into amyloid fibrils has been associated with neurodegenerative diseases. Here, we analyze the formation mediated by condensation low-complexity domain hnRNPA1, a involved in Amyotrophic Lateral Sclerosis (ALS). We show that phase separation and fibrillation are connected but distinct processes which independently different regions sequence. By monitoring spatial temporal evolution demonstrate does not occur homogeneously inside...
Biodegradable polymer nanoparticles are an important class of materials used in several applications for their unique characteristics. In particular, the ones stabilized by zwitterionic gaining increased interest medicine as alternative to more common based on poly(ethylene glycol) thanks superior stability and ability avoid both accelerated blood clearance allergic reactions. this work, a novel NPs has been produced, method independently control nanoparticle size, degradation time,...
Polymeric nanoparticles (NPs) are highly engineered nanoemulsions with applications in several fields. The control over NP surface chemistry, size (Dn), and molecular weight (MW) of the polymer they made up plays a paramount role optimization their end-use performance. In this work, theoretical basis to decouple dependence between Dn MW has been presented, an operative way demonstrated via ab initio reversible addition–fragmentation chain transfer (RAFT) emulsion polymerization (AIREP),...
The RAFT copolymerization of oppositely-charged monomers is studied to optimize the composition polyampholytes with an UCST behaviour and nonfouling properties.