A5085760869- Peanut Plant Research Studies
- Coconut Research and Applications
- Plant Virus Research Studies
- Agricultural pest management studies
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Nitrogen and Sulfur Effects on Brassica
- Statistical Methods and Inference
- Lipid metabolism and biosynthesis
- Plant Disease Resistance and Genetics
- Piezoelectric Actuators and Control
- Legume Nitrogen Fixing Symbiosis
- Higher Education and Teaching Methods
- Synthesis and characterization of novel inorganic/organometallic compounds
- CRISPR and Genetic Engineering
- Ideological and Political Education
- Statistical Methods and Bayesian Inference
- Genomics and Phylogenetic Studies
- Agriculture, Soil, Plant Science
- Structural Health Monitoring Techniques
- Light effects on plants
- Protein Hydrolysis and Bioactive Peptides
- GABA and Rice Research
- Aeroelasticity and Vibration Control
- Metabolomics and Mass Spectrometry Studies
Guangdong Academy of Agricultural Sciences
2015-2025
International Crops Research Institute for the Semi-Arid Tropics
2021
North China Institute of Science and Technology
2019-2021
South China Agricultural University
2020
Nankai University
2015-2016
Shanxi University
2007-2015
East China Normal University
2005-2015
Shanghai Institute of Technology
2015
Shanxi University of Traditional Chinese Medicine
2015
Heilongjiang Provincial Academy of Agricultural Sciences
2014
Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil protein. To explore the origins consequences of tetraploidy, we sequenced A. hypogaea genome compared it with related diploid Arachis duranensis ipaensis genomes. We annotated 39 888 A-subgenome genes 41 526 B-subgenome peanut. The subgenomes have evolved asymmetrically, B subgenome resembling ancestral state A undergoing more gene disruption, loss, conversion, transposable...
Significance We present a draft genome of the peanut A-genome progenitor, Arachis duranensis , providing details on total genes in genome. Genome analysis suggests that lineage was affected by at least three polyploidizations since origin eudicots. Resequencing synthetic tetraploids reveals extensive gene conversion their formation human hands. The A. provides major source candidate for fructification, oil biosynthesis, and allergens, expanding knowledge understudied areas plant biology...
Summary Single‐cell RNA‐seq (scRNA‐seq) has been highlighted as a powerful tool for the description of human cell transcriptome, but technology not broadly applied in plant cells. Herein, we describe successful development robust protoplast isolation system peanut leaf. A total 6,815 single cells were divided into eight clusters based on reported marker genes by applying scRNA‐seq. Further, pseudo‐time analysis was used to developmental trajectory and interaction network transcription...
Summary Although the regulatory mechanisms of dark and light‐induced plant morphogenesis have been broadly investigated, biological process in peanuts has not systematically explored on single‐cell resolution. Herein, 10 cell clusters were characterized using scRNA‐seq‐identified marker genes, based 13 409 11 296 single cells from 1‐week‐old peanut seedling leaves grown under light conditions. 6104 genes 50 transcription factors (TFs) displayed significant expression patterns distinct...
Background Germin-like superfamily members are ubiquitously expressed in various plant species and play important roles development defense. Although several GLPs have been identified peanut (Arachis hypogaea L.), their defense remain unknown. In this research, we study the spatiotemporal expression of AhGLPs functions Results We three new AhGLP (AhGLP3b, AhGLP5b AhGLP7b) that distinct but very closely related DNA sequences. The spatial temporal profiles revealed each GLP gene has its...
Summary The failure of peg penetration into the soil leads to seed abortion in peanut. Knowledge genes involved these processes is comparatively deficient. Here, we used RNA ‐seq gain insights transcriptomes aerial and subterranean pods. More than 2 million transcript reads with an average length 396 bp were generated from one ( AP ) two SP 1 2) pod libraries using pyrosequencing technology. After assembly, sets 49 632, 952 50 494 a total 74 974 assembly contigs TAC s) identified , 2,...
Peanut (Arachis hypogaea L.), an important leguminous crop, is widely cultivated in tropical and subtropical regions. allotetraploid, having A B subgenomes that maybe have originated its diploid progenitors Arachis duranensis (A-genome) ipaensis (B-genome), respectively. We previously sequenced the former here present draft genome of latter, expanding our knowledge unique biology Arachis. The assembled A. ~1.39 Gb with 39,704 predicted protein-encoding genes. gene family analysis revealed...
<title>Abstract</title> Pegging is a unique characteristic of peanut plants in which chasmogamous flowers are produced aerially that penetrate into the soil and develop subterranean pods. However, gene networks regulate peg development remain largely unknown. To investigate spatiotemporal regulation regulatory during growth development. Here, we constructed single-nucleus resolution transcriptomic atlas both aerial pegs comprising 50,903 individual cells annotated nine major cell types using...
Summary Peanut stem is a vital organ to provide mechanical support and energy for aerial tissue development. However, the transcriptional regulatory mechanisms underlying development at single‐cell resolution remain unclear. Herein, single‐nuclei isolation coupled with fluorescent‐activated cell sorting was employed construct atlas of peanut seedling stems using microdroplets‐based RNA‐sequencing. This approach yielded 29 308 cells 53 349 expressed genes identification five types...
A characteristic feature of peanut is the subterranean fructification, geocarpy, in which gynophore ('peg'), a specialized organ that transitions from upward growth habit to downward outgrowth upon fertilization, drives developing pod into soil for subsequent development underground. As step towards understanding this phenomenon, we explore developmental dynamics transcriptome at 11 successive stages. We identified 110 217 transcripts across stages and quantified their abundance along...
Many large-effect quantitative trait loci (QTLs) for yield and disease resistance related traits have been identified in different mapping populations of peanut (Arachis hypogaea L.) under multiple environments. However, only a limited number QTLs used marker-assisted selection (MAS) because unfavorable epistatic interactions between genetic backgrounds. Thus, it is essential to identify consensus across environments backgrounds use MAS. Here, we QTL meta-analysis set peanut.A new integrated...
Peanuts with high oleic acid content are usually considered to be beneficial for human health and edible oil storage. In breeding practice, peanut lines monounsaturated fatty acids selected using desaturase 2 (FAD2), which is responsible the conversion of (C18:1) linoleic (C18:2). Here, comparative transcriptomics were used analyze global gene expression profile high- normal-oleic cultivars at six time points during seed development. First, mutant type FAD2 was determined in high-oleic...
Modern peanut contains fatty acid desaturase 2 (FAD2) mutation, which is capable of producing high oleic for human health. However, the dynamic changes lipidome regarding fad2 remain elusive in seed. In present study, 547 lipid features were identified high- and normal-oleic seeds by utilizing mass spectrometric approach. The fad2-induced differently expressed lipids (DELs) polarly distributed at early maturation stages during high-oleic (OA) seed development. Subsequently, integration...
The peanut is a special plant for its aerial flowering but subterranean fructification. failure of peg penetration into the soil leads to form pod and finally seed abortion. However, mechanism abortion during development remains obscure. Here, comparative transcriptome analysis between pods at different developmental stages was produced using customized NimbleGen microarray representing 36,158 unigenes. By comparing 4 consecutive time-points, totally 6,203 differentially expressed genes,...
Peanuts (Arachis hypogaea L.) are an important oilseed crop, containing high contents of protein and fatty acids (FA). The major components FA found in peanut oil unsaturated FAs, including oleic acid (OA, C18:1) linoleic (LOA, C18:2). Moreover, the content OA is beneficial for human health long-term storage due to its antioxidant activity. However, dynamic changes proteomics related accumulation during seed development still remain largely unexplored. In present study, a comparative...
Microsatellites, or simple sequence repeats (SSRs), represent important DNA variations that are widely distributed across the entire plant genome and can be used to develop SSR markers, which then conduct genetic analyses molecular breeding. Cultivated peanut (A. hypogaea L.), an oil crop worldwide, is allotetraploid (AABB, 2n = 4× 40) species. Because of its complex genome, genomic marker development has been very challenging. However, sequencing cultivated allowed us markers construct a...
Fatty Acid Desaturase 2 (FAD2) controls the conversion of oleic acids into linoleic acids. Mutations in FAD2 not only increase high-oleic content, but also repress leaf growth. However, mechanism by which regulates growth pathway has been elucidated peanut leaves with single-cell resolution. In this study, we isolated fad2 mutant protoplast cells to perform RNA sequencing. Approximately 24,988 individual 10,249 expressed genes were classified five major cell types. A comparative analysis...
Abstract The morphological and molecular diversity of 101 peanut varieties from South China were analyzed to identify distinctness among these varieties. No significant difference was observed for six characteristics whereas a range 0.25–0.51 index 11 characteristics, with an average value 0.39. Molecular characterization 40 highly polymorphic simple sequence repeats (SSRs) generated total 167 alleles ranging two per marker 4.18 marker. polymorphism information content (PIC) markers varied...
The far-red-impaired response 1 (FAR1) transcription family were initially identified as important factors for phytochrome A (phyA)-mediated far-red light signaling in Arabidopsis; they play crucial roles controlling the growth and development of plants. reported reference genome sequences Arachis, including A. duranensis, ipaensis, monticola, hypogaea, its related species Glycine max provide an opportunity to systematically perform a genome-wide identification FAR1 homologous genes...