A5077573264- Photoreceptor and optogenetics research
- Light effects on plants
- Traumatic Brain Injury and Neurovascular Disturbances
- SARS-CoV-2 and COVID-19 Research
- Cardiac electrophysiology and arrhythmias
- bioluminescence and chemiluminescence research
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- ATP Synthase and ATPases Research
- Neuroscience and Neural Engineering
- Respiratory viral infections research
- Advanced Neuroimaging Techniques and Applications
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Protein Degradation and Inhibitors
- Traumatic Brain Injury Research
- Sepsis Diagnosis and Treatment
- S100 Proteins and Annexins
- Neonatal Respiratory Health Research
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
Columbia University
2020-2024
Uniformed Services University of the Health Sciences
2016-2023
We report a novel method for the transient expression of SARS-CoV-2 envelope (E) protein in intracellular organelles and plasma membrane mammalian cells Xenopus oocytes. Intracellular E increases intra-Golgi pH. By targeting to membrane, we show that it forms cation channel, viroporin, is modulated by changes This studying activity viroporins may facilitate screening new antiviral drugs identify treatments COVID-19.The coronaviruses such as SARS-CoV-1 proposed form an ion channel or...
Abstract Repeated mild traumatic brain injuries (mTBI) may lead to serious neurological consequences, especially if re-injury occurs within the period of increased cerebral vulnerability (ICV) triggered by initial insult. MRI and blood proteomics might provide objective measures pathophysiological changes in mTBI indicate when is no longer a state ICV. This study assessed behavioral, blood-based markers rat model mTBI. Rats were given sham or fluid percussion injury (mFPI) behavioral...
Abstract Optogenetic genome engineering tools enable spatiotemporal control of gene expression and provide new insight into biological function. Here, we report the version genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0. To improve technology, compare light-dimerization optimize for mammalian using a CAG promoter, Magnets, 2A self-cleaving peptide. prevent background recombination caused by high sequence similarity in dimerization domains, modify codons mouse targeting...
Lactate metabolism has been shown to have increasingly important implications in cellular functions as well the development and pathophysiology of disease. The various roles a signaling molecule metabolite led interest establishing new method detect lactate changes live cells. Here we report our genetically encoded metabolic indicator specifically for probing (GEM-IL) based on superfolder fluorescent proteins mutagenesis. With improvements its design, specificity, sensitivity, GEM-IL allows...
Monitoring protein biomarker levels in the cerebrospinal fluid (CSF) can help assess injury severity and outcome after traumatic brain (TBI). Determining injury-induced changes proteome of extracellular (bECF) more closely reflect parenchyma, but bECF is not routinely available. The aim this pilot study was to compare time-dependent S100 calcium-binding B (S100B), neuron-specific enolase (NSE), total Tau, phosphorylated Tau (p-Tau) matching CSF samples collected at 1, 3, 5 days post-injury...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has affected approximately 800 million people since the start of Disease 2019 (COVID-19) pandemic. Because high rate mutagenesis in SARS-CoV-2, it is difficult to develop a sustainable approach for prevention and treatment. The Envelope (E) protein highly conserved among human coronaviruses. Previous studies reported that SARS-CoV-1 E deficiency reduced viral propagation, suggesting inhibition might be an effective therapeutic...
Abstract Red light is useful for optogenetic control of various protein activities in mammalian deep tissues due to its high tissue penetration, low invasiveness, and scattering. However, technology that enables the manipulation using red remains elusive. Here we develop a light-activatable, semi-synthetic photoswitch, named MagRed. MagRed composed light-absorbing bacterial phytochrome incorporating endogenous chromophore, biliverdin, photo-state-specific de novo synthetic binder. allows us...