A5014032771- Glycosylation and Glycoproteins Research
- Rabbits: Nutrition, Reproduction, Health
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Protein purification and stability
- Pharmacological Effects of Natural Compounds
- Animal Ecology and Behavior Studies
- Animal Virus Infections Studies
- Genetic Mapping and Diversity in Plants and Animals
- Viral gastroenteritis research and epidemiology
- Chromosomal and Genetic Variations
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Blood groups and transfusion
- Genetic diversity and population structure
- Toxin Mechanisms and Immunotoxins
- Immune Response and Inflammation
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Immunodeficiency and Autoimmune Disorders
- Wildlife Ecology and Conservation
- interferon and immune responses
- Animal Disease Management and Epidemiology
- Vector-borne infectious diseases
- Hepatitis B Virus Studies
Universidade do Porto
2006-2019
Centro de Investigação em Biodiversidade e Recursos Genéticos
2007-2011
Vrije Universiteit Brussel
1993-2009
Folkhälsans Forskningscentrum
2009
Instituto Nacional de Investigação Agrária e Veterinária
2008
University of East Anglia
1999
Vlaams Instituut voor Biotechnologie
1999
U.S. National Science Foundation
1983
Karolinska Institutet
1982
Stanford University
1977-1979
B lymphocytes capable of generating primary IgM and IgG plaque-forming cells (PFC) responses to burro erythrocytes have surface IgD, as do PFC. memroy arising after one injection antigen are divided into two groups, which expresses IgD while the other has no detectable membrane IgD. PFC generated from memory lacking show a higher average avidity than those IgD-positive cells, indicating that mature not After more antigen, few, if any, in or secondary immune response lack membrane-bound These...
Abstract A rabbit of allotype a1a3, b4b5 was hyperimmunized with lyophilized Micrococcus lysodeikticus. Two weeks after the appearance antibodies restricted heterogeneity, serum immunoglobulins this animal reacted anti-a1, anti-a2, anti-a3, and anti-b4, anti-b5, anti-b6 antisera. Control experiments showed that reaction specific for allotypic determinants cross-reactions could be excluded. Isolated light chains were shown to contain b4, b5, b6 specificities. Serial bleedings indicated all...
Lentiviruses are causal agents of severe pathologies a variety mammals, including cattle and humans (e.g., AIDS different types lymphoma). While endogenous forms lentivirus do not occur in these species, A. Katzourakis coworkers (A. Katzourakis, M. Tristem, O. G. Pybus, R. J. Gifford, Proc. Natl. Acad. Sci. USA 104:6261-6265, 2007) recently reported the presence genome European rabbit (Oryctolagus cuniculus) multiple sequences defining lentiviral subgroup elegantly referred to as RELIK...
Lymphocyte precursors originating in the bone marrow undergo several antigen-independent differentiational steps that result commitment both to eventual function and antigen reactivity. Exposure of these committed induces further differentiation, either directly effector cells or memory capable rapid expansion differentiation upon reexposure antigen. The latter stages pathways are regulated by other lymphocytes which act singly concert govern extent overall response. Taken together,...
Abstract Mouse spleen cells were cultured with lipopolysaccharide in conditions that activate both IgM and IgG secretion. Addition of cytochalasin B (CB), an inhibitor cytokinesis, lead to a high degree polynucleation, little effect on Ig Using cytoplasmic staining fluorochrome conjugated antisera, we determined the numbers IgG-containing also contained their cytoplasm. Such double relatively more frequent at early times cultures, but all single producing majority. CB over period when first...
Abstract The minimal gene diversity at a locus of the antibody constant region, as estimated in natural populations rabbit, revealed levels heterozygosity similar to those reported for major histocompatibility complex human and murine populations. Sera 416 wild rabbits were collected on Iberian peninsula three islands Azorean archipelago analyzed occurrence serological markers b immunoglobulin light chain. All four serotypes present domestic found Portugal. They represented less than 50%...
Abstract Anti‐a 2 sera raised in a3 rabbits are shown to detect determinants on a2 molecules which different from those detected by anti‐a al animals. The former occasionally observed at a low level of the allotype and high Leporidae genera. two types compete for same sterical region molecules. All homogeneous have been tested show both determinants.
The morphology, testis histology, mitosis, meiosis and genetically determined immunoglobulin markers were studied in an artificially bred hybrid male of Lepus timidus europaeus.The overall appearance the hare resembled that timidus, but it had some morphological features Lepics europaeus as well.The karyotype was identical with both parental species, testes showed normal spermatogenetic activity, meiotic divisions.The serum from this reacted antiserum specific for a marker immunoglobulins.A...
Abstract Background Since the first report of antiretroviral restriction factor TRIM5α in primates, several orthologs other mammals have been described. Recent studies suggest that leporid retroviruses like RELIK, reported endogenous lentivirus ever, may imposed positive selection European rabbit and brown hare. Considering RELIK must already present a common ancestor genera Lepus , Sylvilagus Oryctolagus we extended study evolutionary patterns to members Leporidae family, particularly genus...
Bactericidal effects of polylysine on different bacterial species were measured. Marked differences in sensitivity observed. Based the concentration required to reduce cell viability by 50%, Mycobacterium smegmatis and tuberculosis found be most sensitive Escherichia coli resistant. In addition, two Gram-positive organisms, Staphylococcus epidermidis Streptococcus salivarius exhibited significant which suggests that relationship between towards type is not necessarily a function overall...
Abstract The large interallelic distances between the three rabbit Ig VHa lineages, a1, a2 and a3, suggest that persistence time of polymorphism could amount to 50 million years, which is much longer than MHC polymorphisms. Rabbit originated in Iberian Peninsula where two subspecies coexist, one confined Southwestern Iberia (Oryctolagus cuniculus algirus). We studied VH loci original species range obtain a better understanding evolutionary history this unusual polymorphism. Serological...