Objective. To evaluate qualitative and quantitative composition of the wall intestinal microbiota healthy young people living in Saint-Petersburg with gas chromatography — mass spectrometry method. Design Methods. The study involved 116 (66 men 50 women), constantly SaintPetersburg. age examined were 20-35 years old. Quantitative was performed by analysis blood spectrometry. Results conclusions . GC-MS method allows to detect markers, components cells a wide range microorganisms samples...

The paper presents preliminary results of a comparative study assessing the gut microbiota in patients with Parkinson's disease and control group using gas chromatography mass spectrometric detection. Sixteen stage 3 94 age-matched persons without were examined. It was revealed that total number microbial markers parietal intestinal increased by 43% compared group. This increase is due to 2-fold conditional-pathogenic flora, at same time there decrease useful microflora. obtained may be...

Identification of workers who are at high risk and low as a result participation in the cleanup consequences accident Chernobyl nuclear power station ("liquidators") is important both for scientific research medical care individuals involved. Using register data 36,700 liquidators, authors estimated relationships between radiation doses following factors: time arrival zone, irradiation conditions, possible health effects exposures. The analyses took into account types work (decontamination,...

Zinc content in blood plasma and brain tissue of rats was studied by analytic mass-spectrometry with inductively coupled plasma. In control (saline-treated animal) zinc 3.6±1.4 mg/ml, the liver – 12.5±2.5 mg/mg, spleen 10.9±4.1 8.7±3.0 mg/mg. After a single intraperitoneal injection donator acizolum (24 mg/kg) decreased all examined tissues, especially brain. course sequential injections (seven administrations during two weeks) essential elevation tissues investigated detected. The maximal...

The presented study is devoted to one of the urgent problems conservative treatment chronic pancreatitis — relief painful abdominal and dyspeptic syndromes. Polymorphism etiopathogenetic variants formation pancreatic diseases naturally justifies need for complex therapy, including secretolytics, antispasmodics medications polyenzyme drugs. use Meteospasmyl in therapy effectively relieves pain dyspepsia, modifies composition intestinal microbiota improves quality life patients with pancreatitis.

Relevance. Professional activities of firefighters and rescuers are usually associated with aggressive chemical exposure increased physical exertion psychological stress. Therefore, for professional selection monitoring the health status EMERCOM Russia it is necessary to evaluate activity cytochrome P450 isoenzymes that directly involved in detoxification pathways liver. Intention. To CYP3A4 enzyme by phenotyping methods establish frequency allelic variants gene this (rs2740574 rs4987161...

Relevance. Professional activity of operational staff the Russian EMERCOM in Arctic zone is associated with adaptive changes body resulting all types metabolism, appearance functional abnormalities and, without proper correction, development various diseases. The complex system metabolic interaction between humans and microbiota well described by “microbiota-gut-brain” axis, which includes endocrine, immune, neurohumoral pathways. Dysfunction this axis may be involved pathogenesis diseases...

Fludarabine is a purine antimetabolite with pronounced immunosuppressive effect. The inhibitory effect of fludarabine depends on its concentration in blood plasma. In addition, the phenotypic characteristics patients affect pharmacokinetic and pharmacodynamic profile drug, which necessitates personalized approach to dosage regimen. chromatography-mass spectrometric method for quantitative determination 2-fluorine plasma was developed studying individual parameters pharmacokinetics...

Introduction . Factors affecting the severity of course a new coronavirus infection remain unexplored to end Aim To study factors associated with transition COVID-19 from moderate severe form disease in patients undergoing inpatient treatment Materials and methods The diagnosis COVID-19-associated pneumonia was made by clinician on basis PCR test data an up-to-date chest CT scan. A total 195 consecutively recruited were examined. average age surveyed 54 (12.1) years. Results Significantly...

Relevance . Individuals of stressful professions, including the emergency response officers (firefighters and rescuers) Ministry Emergency Situations Russia, are prone to early onset circulatory system diseases (CSD). At in-depth examinations CSD detection rate in EMERCOM is above 10%. Earlier studies report that risk factors widespread among Russia officers. Therefore, it relevant identify predisposition as soon possible order maintain good health longevity highly qualified professionals...

Since 2020, the world has been living in COVID-19 pandemic caused by SARS-CoV-2 virus. This situation is due to high virulence and prevalence of virus, which reached epidemic proportions recent years. Hemostasis disorders are main marker prognosis severe course disease. The virus body a patient with triggers pathophysiological chain reaction, which, turn, will lead development cytokine storm, disorder hemostasis system, multiple organ disorders. purpose study: determine study influence...

Background. The undesirable side effects in the process of pharmacotherapy are forced to look for ways prevent them. most effective means this is carry out individual therapeutic drug monitoring. This especially true patients who undergo chemotherapy. A narrow range antineoplastic agent concentrations requires an approach management each patient. purpose study justify need monitoring provide safety antitumor drugs. Methods. Methods quantitative determination active metabolite fludarabine,...

Relevance. Due to their carcinogenic, oxidative and toxic potential, chemical compounds from burning products are especially dangerous for rescuers firefighters result in the stress as a precursor of many diseases. Therefore, it is necessary diagnose before its serious effects. Intention . To assess markers plasma EMERCOM Russia via modern methods targeted treatment preventive actions. Methodology Malonic dialdehyde, fat-soluble vitamins (A E), polyunsaturated omega-3 omega-6 fatty acids...

Relevance. Increasing prevalence of concomitant pathology among the Chernobyl accident recovery workers is associated with long-term adverse health effects. Due to a significant role dyslipidemia, microbiological disorders gastrointestinal tract should be assessed along individual indicators cholesterol metabolism. Intention. To study features gut microbiome composition in workers. Methods. Lipid metabolism was based on total blood serum. Parietal intestinal microbiota studied via gas...

A high demand for sildenafil-based drugs puts a premium on the development of methods quantitation sildenafil in bio-substrates to facilitate pharmacokinetic analysis bioequivalence studies. High performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was proposed method due its sensitivity, selectivity, reproducibility, and performance. The aim study develop validate an HPLC-MS/MS quantitative determination active metabolite N-desmethyl human plasma. Approbation...

Relevance. Today gastric cancer is still one of the oncologic diseases most often leading to death. H. pylori eradication reduces risk cancer, but its efficacy depends on mucosa state. Atrophy more common in Chernobyl nuclear power plant (CNPP) accident recovery workers than patients who have not been involved CNPP works. It seems especially important investigate features antibiotics transport colonization area this contingent. Intention – determine amoxicillin secretion by with atrophic and...

AIM: The aim was to assess systemic transport of amoxicillin, the most common antibiotic in H. pylori eradication regimens gastric atrophic gastritis.
 MATERIALS AND METHODS: Systemic amoxicillin lumen rats evaluated washes from mucosa model gastritis after intravenous drug infusion. Transport bloodstream also assessed patients with and non-atrophic aspirated via nasogastric probe juice oral administration. Amoxicillin concentration measured samples using liquid chromatography-mass...

Based on a long-term dynamic study of the state health liquidators consequences Chernobyl accident, it was found that 75 % patients have metabolic syndrome, burdened with concomitant somatic pathology. The article presents literature data key role adipokine levels in development syndrome adipose tissue, hormonal indicators, vitamins and other nutrients, which require modern methods laboratory diagnostics to determine. aim. To develop clinical algorithm for diagnosing accident using new...