A5048197627- Alzheimer's disease research and treatments
- Supramolecular Self-Assembly in Materials
- Parkinson's Disease Mechanisms and Treatments
- Nerve injury and regeneration
- Hydrogels: synthesis, properties, applications
- Graphene and Nanomaterials Applications
- Retinal Imaging and Analysis
- RNA Interference and Gene Delivery
- Protein Structure and Dynamics
- Prion Diseases and Protein Misfolding
- biodegradable polymer synthesis and properties
- Neurogenesis and neuroplasticity mechanisms
- Sinusitis and nasal conditions
- Ophthalmology and Visual Impairment Studies
- Conducting polymers and applications
- Graphene research and applications
- Infectious Aortic and Vascular Conditions
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Ear Surgery and Otitis Media
- Neurological diseases and metabolism
- Cardiovascular and Diving-Related Complications
- Respiratory and Cough-Related Research
- Ophthalmology and Eye Disorders
- Artificial Intelligence in Healthcare
Indian Institute of Technology Bombay
2014-2021
North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences
2021
IITB-Monash Research Academy
2014-2018
Materials Science & Engineering
2017
Monash University
2017
Madurai Kamaraj University
2017
Abstract Amyloids are cross-β-sheet fibrillar aggregates, associated with various human diseases and native functions such as protein/peptide hormone storage inside secretory granules of neuroendocrine cells. In the current study, using amyloid detecting agents, we show that growth (GH) could be stored in pituitary rat. Moreover, to demonstrate formation GH vitro , studied conditions (solvents, glycosaminoglycans, salts metal ions) found presence zinc ions (Zn(II)), formed short curvy...
We report a new class of amyloid-inspired peptide hydrogels that was designed and based on α-synuclein protein for which hydrogel formation is triggered by various stimuli, such as heating/cooling or changes in pH. The peptides resemble cross-β-sheet-rich amyloid, they assemble into nanofibrous meshwork mimics the natural extracellular matrix. Our design principle allows easy manipulation gelator sequence to exploit desirable properties amyloids use cell replacement therapies...
The storage of protein/peptide hormones within subcellular compartments and subsequent release are crucial for their native function, hence these processes intricately regulated in mammalian systems. Several peptide were recently suggested to be stored as amyloids endocrine secretory granules. This leads an apparent paradox where requires formation aggregates, function a supply non-aggregated peptides on demand. precise mechanism behind amyloid by remain open question. To address this, we...
Abstract Alpha-synuclein (α-Syn) aggregation into oligomers and fibrils is associated with dopaminergic neuron loss occurring in Parkinson’s disease (PD) pathogenesis. Compounds that modulate α-Syn interact preformed fibrils/oligomers convert them to less toxic species could have promising applications the drug development efforts against PD. Curcumin one of Asian food ingredient which showed role as therapeutic agent many neurological disorders including However, instability low solubility...
The involvement of α-synuclein (α-Syn) amyloid formation in Parkinson's disease (PD) pathogenesis is supported by the discovery α-Syn gene (SNCA) mutations linked with familial PD, which are known to modulate oligomerization and aggregation α-Syn. Recently, A53V mutation has been discovered, leads late-onset PD. In this study, we characterized for first time biophysical properties A53V, including propensities, toxicity aggregated species, membrane binding capability, along those all at A53...
Amyloid based hydrogels can mimic the extracellular matrix and serve as matrices for tissue engineering both in vitro vivo. A pH responsive self‐assembled amyloid hydrogel system is used to encapsulate various growth factors driving stem cell differentiation toward neuronal lineage. Diffusion studies with fluorescence recovery after photobleaching bulk release model protein fluorescein isothiocyanate‐bovine serum albumin show that encapsulated molecules be released a sustained fashion from...
Abstract α‐Synuclein (α‐Syn) aggregation is associated with Parkinson's disease (PD) pathogenesis. In PD, the role of oligomers versus fibrils in neuronal cell death debatable, but recent studies suggest are a proximate neurotoxin. Herein, we show that soluble α‐Syn monomers undergo transformation from solution to gel state on incubation at high concentration. Detailed characterization showed coexistence monomers, oligomers, and short fibrils. vitro, was highly cytotoxic human neuroblastoma...
It has been suggested that conjugated charged polymers are amyloid imaging agents and promising therapeutic candidates for neurological disorders. However, very less is known about their efficacy in modulating the aggregation pathway. Here, we studied modulation of Parkinson's disease associated α-synuclein (AS) assembly kinetics using polyfluorene (PF, cationic; PFS, anionic). We also explored complexation these with various AS aggregated species including fibrils oligomers...
α-Synuclein (α-Syn) aggregation and amyloid formation are associated with loss of dopaminergic neurons in Parkinson's disease (PD). In addition, familial mutations α-Syn shown to be one the definite causes PD. Here we have extensively studied PD G51D, H50Q, E46K using Drosophila model system. Our data showed that flies expressing mutants a shorter lifespan exhibit more climbing defects compared wild-type (WT) an age-dependent manner. The immunofluorescence studies brain from old neuronal...
Amyloid formation of α-synuclein (α-Syn) and its familial mutations are directly linked with Parkinson's disease (PD) pathogenesis. Recently, a new α-Syn mutation (A53E) was discovered, associated an early onset aggressive form PD, which delays aggregation. When we overexpressed wild-type (WT) A53E proteins in cells, showed neither toxicity nor aggregate formation, suggesting merely overexpression may not recapitulate the PD phenotype cell models. We hypothesized that cells expressing mutant...
Abstract Graphene‐based hybrid nanomaterials have been shown to great potential in various biotechnology applications including enzyme sensing and bone tissue engineering. Harnessing the unique properties of graphene material strength amyloids, a graphene–amyloid composite film is developed that can self‐organize into periodic troughs crests without need lithographic techniques or etching. The topographies generated by provide powerful modulators contact guidance neural precursor cells,...
Amyloid fibrils are cross-β-sheet-rich protein/peptide that typically associated with neurodegenerative diseases such as Parkinson's and Alzheimer's disease. Recently, functional amyloids have been discovered where implicated in performing normal physiological functions of the host organism rather than creating diseases. The ability to interact cell membrane other small biomolecules exhibits its great potential be used a biomaterial for adhesion gene delivery system. Given established...
The master regulator, DnrI of Streptomyces peucetius is a member the family transcriptional activator, antibiotic regulatory proteins (SARP), which controls biosynthesis antitumor anthracycline, daunorubicin (DNR) and doxorubicin (DXR). binding to heptameric repeat sequence found within −35 promoter region biosynthetic gene, dpsE activates it. To combat increased level intracellular DNR, cell has developed self resistance mechanism mediated by drrAB drrC genes are regulated genes. We find...
This paper presents data related to the research article "Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem differentiation" [1]. Here we probed collective influence all-trans retinoic acid (RA) substrate properties (amyloid hydrogel) on human mesenchymal (hMSC) differentiation. Stem cells were cultured soft [1], [2] in presence absence matrix encapsulated RA. The morphology was imaged assessed via quantification circularity. Further immunostaining...
Amyloid fibrils are structurally heterogeneous protein aggregates that implicated in a wide range of neurodegenerative and other proteopathic diseases. These exist variety different tertiary higher-level structures, this exhibited polymorphism greatly complicates any structural study amyloid fibrils. In work, we demonstrate method using polarization-resolved microscopy to directly observe the heterogeneity individual amyloid-bound fluorophores. We formulate mathematical quantity, helical...
<p class="abstract"><strong>Background:</strong> Biofilm is an organized bacterial community that may be homogeneous or heterogeneous. It plays a significant role in the pathogenesis of chronic rhinosinusitis, tonsillitis and suppurative otitis media. Biofilms explain recurrence recalcitrant episodes otorrhea media (COM). The objective this study was to detect biofilm formation compare pattern tubotympanic atticoantral ear diseases.</p><p...
Abstract α‐Synuclein (α‐Syn) aggregation is associated with Parkinson's disease (PD) pathogenesis. In PD, the role of oligomers versus fibrils in neuronal cell death debatable, but recent studies suggest are a proximate neurotoxin. Herein, we show that soluble α‐Syn monomers undergo transformation from solution to gel state on incubation at high concentration. Detailed characterization showed coexistence monomers, oligomers, and short fibrils. vitro, was highly cytotoxic human neuroblastoma...
Samir K. Maji and co-workers present an amyloid hydrogel for sustained growth factors delivery in tissue engineering applications article number 1700368. The image represents stem cells (blue spheres) embedded within the matrix (grey network) encapsulating (green dots). Within 3D culture, self-organizes into spheroids (red sphere) with up-regulated neural genes. In three-dimensional cell entrapped surface property of amyloids promoted neuro-sphere formation higher neuronal gene expression.
The identification of a person who committed the crime by witness was an integral part in some forensic cases. perfection eye sight, free from diseases play important role investigation, otherwise it may end with false identification. Glaucoma is group ocular condition involving progress visual loss optic nerve damage leading to blindness if left untreated. This study investigates normative data anterior chamber depth (ACD), angle (ACA) and other parameters 90 patients Adriel Eye Health,...