A5076368985- Genetics and Neurodevelopmental Disorders
- Cholesterol and Lipid Metabolism
- RNA modifications and cancer
- Skin and Cellular Biology Research
- Genomics and Chromatin Dynamics
- Autism Spectrum Disorder Research
- Receptor Mechanisms and Signaling
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Liver Disease Diagnosis and Treatment
- Electronic Health Records Systems
- Drug Transport and Resistance Mechanisms
- Hepatitis B Virus Studies
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Cancer Genomics and Diagnostics
- Pediatric Hepatobiliary Diseases and Treatments
- Telemedicine and Telehealth Implementation
- Digestive system and related health
- Mathematical Biology Tumor Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
McGill University Health Centre
2021-2025
Université de Sherbrooke
2019-2024
McGill University
2016-2023
Centre hospitalier universitaire de Québec
2010
Université Laval
1996-2010
Hôtel-Dieu de Québec
1996-2008
Quebec - Clinical Research Organization in Cancer
2006
The alpha1-fetoprotein (AFP) gene is located between the albumin and alpha-albumin genes activated by transcription factor FTF (fetoprotein factor), presumed to transduce early developmental signals cluster. We have identified as an orphan nuclear receptor of Drosophila FTZ-F1 family. recognizes DNA sequence 5'-TCAAGGTCA-3', canonical recognition motif for receptors. cDNA homologies indicate that rat ortholog mouse LRH-1 Xenopus xFF1rA. Rodent encoded a single-copy gene, related encoding...
Eaf1 (for Esa1-associated factor 1) and Eaf2 have been identified as stable subunits of NuA4, a yeast histone H4/H2A acetyltransferase complex implicated in gene regulation DNA repair.While both SWI3-ADA2-N-CoR-TF IIIB domain-containing proteins are required for normal cell cycle progression, their depletion does not affect the global Esa1-dependent acetylation histones.In contrast to all other subunits, is found exclusively associated with NuA4 vivo.It serves platform that coordinates...
Keratins are intermediate filament (IF) proteins of epithelial cells, expressed as pairs in a lineage/differentiation manner. Hepatocyte and hepatoma cell IFs made solely keratins 8/18 (K8/K18), the hallmark all simple epithelia. Cell attachment/spreading (adhesion) migration involve formation focal adhesions at sites integrin interactions with extracellular matrix, actin adaptors such talin vinculin, signaling molecules adhesion kinase (FAK) member(s) protein C (PKC) family. Here, we...
PCSK9 (proprotein convertase subtilisin-kexin 9) enhances the degradation of LDLR (low-density lipoprotein receptor) in endosomes/lysosomes. This study aimed to determine sites phosphorylation at Ser-residues and consequences such posttranslational modification on secretion activity LDLR. Approach Results: Fam20C (family with sequence similarity 20, member C) phosphorylates serines secretory proteins containing motif S-X-E/phospho-Ser, including cholesterol-regulating PCSK9. In situ...
Endosomal sorting complexes required for transport (ESCRT) drive cell surface receptor degradation resulting in attenuation of oncogenic signaling and pointing to a tumor suppressor function. Here, we show that loss function an ESCRT protein (HD-PTP encoded by the PTPN23 gene, located on gene cluster 3p21.3) drives tumorigenesis vivo. Indeed, Ptpn23+/− predisposes mice sporadic lung adenoma, B lymphoma, promotes Myc-driven lymphoma onset, dissemination, aggressiveness. Ptpn23+/−-derived...
Abstract CCDC88B is a risk factor for several chronic inflammatory diseases in humans and its inactivation causes migratory defect DCs mice. belongs to family of cytoskeleton-associated scaffold proteins that feature protein:protein interaction domains. Here, we identified the Rho/Rac Guanine Nucleotide Exchange Factor 2 (ARHGEF2) RAS Protein Activator Like 3 (RASAL3) as physical functional interactors. Mice defective Arhgef2 or Rasal3 show dampened neuroinflammation, display altered...
Objective: To build and validate a simulation framework to perform single-cell bulk-cell whole genome sequencing of radiation-exposed Monte Carlo cell models assist radiation genomics studies. Approach: Sequencing the genomes radiation-damaged cells can provide useful insight into action for radiobiology research. However, carrying out post-irradiation experiments often be challenging, expensive, time-consuming. Although computational simulations have potential solutions these experimental...
Fragile X Syndrome (FXS) is caused by mutations in the fragile mental retardation 1 gene, characterized low plasma cholesterol levels. Considering essential role of brain signaling and synaptogenesis, it important to screen for abnormalities FXS explore their link with neuropsychological profiles. Brain synthesized situ, excess primarily converted 24(S)-hydroxycholesterol (24(S)-OHC). 27-hydroxycholesterol (27-OHC) major oxidation metabolite that crosses blood-brain barrier from peripheral...
Autism spectrum disorder (ASD) has been associated with low cholesterol levels in a limited number of studies. However, the prevalence hypocholesterolemia as well degree association ASD remains to be elucidated. We therefore sought investigate lipid profiles group French‐Canadian individuals. The medical records 79 individuals and age gender‐matched healthy controls were retrospectively reviewed. fasting including total (TC), high‐density lipoprotein, triglycerides, low‐density lipoprotein...
The estrogen-related receptor alpha (ERRα) is a primary regulator of mitochondrial energy metabolism, function and dynamics, has been implicated in autophagy immune regulation. ERRα abundantly expressed the intestine cells system. However, its role inflammatory bowel disease (IBD) remains unknown. Here, we report protective intestine. We found that mice deficient were susceptible to experimental colitis, exhibiting increased colon inflammation tissue damage. This phenotype was mediated by...
ABSTRACT Orphan nuclear receptor fetoprotein transcription factor (FTF) was previously identified as a specific regulator of the α 1 -fetoprotein gene during early liver development and in response to hormonal signals (L. Galarneau, J.-F. Paré, D. Allard, Hamel, L. Lévesque, J. Tugwood, S. Green, Bélanger, Mol. Cell. Biol. 16:3853–3865, 1996). Here we report functional analysis FTF interactions with hepatitis B virus (HBV) nucleocapsid promoter. DNA-protein-binding assays show that HBV...
Fragile X syndrome (FXS) is the most prevalent monogenic cause of intellectual disability and autism spectrum disorder (ASD). Affected individuals have a high prevalence hypocholesterolemia, however, underlying mechanisms clinical significance remains unknown. We hypothesized that decrease in plasma cholesterol levels associated with an alteration content within lipid rafts (LRs) which ultimately affects profile FXS individuals. The platelets LRs were isolated by ultracentrifugation on...
Review Articles| December 16 1998 Assignment of the fetoprotein transcription factor gene (FTF) to human chromosome band 1q32.11 by in situ hybridization Subject Area: Genetics L. Galarneau; Galarneau aLe Centre de recherche en cancérologie l'Université Laval, hospitalier universitaire Québec, Pavillon L'Hôtel-Dieu; Search for other works this author on: This Site PubMed Google Scholar R. Drouin; Drouin bUnité génétique humaine et moléculaire, Québec,Pavillon Saint-François-d'Assise, Québec...
Accurate quantification of 24(S)-hydroxycholesterol and 27-hydroxycholesterol holds substantial biological significance due to their involvement in pivotal cellular processes, encompassing cholesterol homeostasis, inflammatory responses, neuronal signaling, potential as disease biomarkers. The plasma determination these oxysterols is challenging considering low concentrations similarities terms empirical formulae, molecular structure, physicochemical properties across all human endogenous...
Abstract Purpose Radiotherapy patients often face undue anxiety due to misconceptions about radiation and their inability visualize upcoming treatments. Access personal treatment plans is one way in which pre‐treatment may be reduced. But radiotherapy data are quite complex, requiring specialized software for display necessitating personalized explanations understand them. Therefore, our goal was design implement a novel menu patient portal improve access understanding of plans. Methods A...
Abstract High plasma matrix metalloproteases-9 (MMP-9) levels have been reported in Fragile X Syndrome a limited number of animal and human studies. Since the results obtained are method-dependent not directly comparable, clinical utility MMP-9 measurement FXS remains unclear. This study aimed to compare quantitative gel zymography ELISA determine which method better discriminates abnormal individuals with from healthy controls correlates profile. The active total forms were quantified...
Orphan nuclear receptor fetoprotein transcription factor (FTF) was previously identified as a specific regulator of the α1-fetoprotein gene during early liver development and in response to hormonal signals (L. Galarneau, J.-F. Paré, D. Allard, Hamel, L. Lévesque, J. Tugwood, S. Green, Bélanger, Mol. Cell. Biol. 16:3853–3865, 1996). Here we report functional analysis FTF interactions with hepatitis B virus (HBV) nucleocapsid promoter. DNA-protein-binding assays show that HBV core promoter...