A5101705228- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Genetics and Plant Breeding
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Crop Yield and Soil Fertility
- Plant Disease Resistance and Genetics
- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Biological and pharmacological studies of plants
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Medicinal Plants and Neuroprotection
- GABA and Rice Research
- Soybean genetics and cultivation
- Nematode management and characterization studies
- Plant Reproductive Biology
- Wheat and Barley Genetics and Pathology
- Potato Plant Research
- Genetic and phenotypic traits in livestock
- Plant responses to elevated CO2
Sichuan Agricultural University
2010-2024
Ministry of Agriculture
2021
The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant (BZR1) transcription factors, key components in the brassinosteroid signaling pathway, play pivotal roles plant growth and development. However, function of BES1/BZR1 crops during stress response remains poorly understood. In present study, we characterized ZmBES1/BZR1-5 from maize, which was localized to nucleus responsive abscisic acid (ABA), salt drought stresses. Heterologous expression transgenic Arabidopsis resulted decreased ABA...
Abstract The BES1/BZR1 transcription factors regulate the expression of genes responsive to brassinosteroids and play pivotal roles in plant development, but their role regulating kernel development maize remains unclear. In this study, we found that ZmBES1/BZR1-5 positively regulates size. Association analysis candidate 513 diverse inbred lines indicated three SNPs related were significantly associated with width whilst four 100-kernel weight. Overexpression Arabidopsis rice both increased...
Serine/threonine protein phosphatase 2C (PP2C) dephosphorylates proteins and plays crucial roles in plant growth, development, stress response. In this study, we characterized a clade B member of maize PP2C family, i.e., ZmPP2C26, that negatively regulated drought tolerance by dephosphorylating ZmMAPK3 ZmMAPK7 maize. The ZmPP2C26 gene generated ZmPP2C26L ZmPP2C26S isoforms through untypical alternative splicing. lost 71 amino acids including an MAPK interaction motif showed higher activity...
The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1(BZR1) transcription factors play crucial roles in plant growth, development, and stress response. However, little is known about the function of maize's BES1/BZR1s. In this study, ZmBES1/BZR1-3 ZmBES1/BZR1-9 genes were cloned from inbred line, B73, they functionally evaluated by analyzing their expression pattern, subcellular localization, transcriptional activation activity, as well heterologous Arabidopsis, respectively. results...
ABSTRACT Banded leaf and sheath blight (BLSB) caused by Rhizoctonia solani Kühn in maize ( Zea mays L.) is an important disease China as well South Southeast Asia. The identification of quantitative trait loci (QTL) for resistance to this would facilitate the development resistant hybrids. A mapping population consisting 229 F 2 individuals derived from cross inbreds R15 (resistant) 478 (susceptible) was used study. genetic linkage map constructed containing 146 single sequence repeat (SSR)...
Zinc is an essential micronutrient for plant growth and development, functions as a cofactor hundreds of transcription factors enzymes in numerous biological processes. deficiency common abiotic stress resulting yield loss quality deterioration crops, but zinc excess causes toxicity systems. In plants, homeostasis tightly modulated by transporters binding compounds that uptake/release, transport, localize, store zinc, well their upstream regulators. Lazarus 1 (LAZ1), member DUF300 protein...
Summary Previous studies found that the browning characteristics of ten potato cultivars were closely related to their ascorbic acid (AsA) content. In order reveal relationship between endogenous AsA and browning, we quantified AsA, dehydroascorbic (DHA). Among seven cultivars, 181–25, 182–9 41–2 had little colour alteration within 48 h, 51–2 39–1 severely browned at fresh‐cut 3 h. A correlation analysis discovered was significantly negatively correlated with total (AsA + DHA), AsA/DHA...
To transfer desirable characters from perennial teosinte [ Zea perennis (Hitchc.) Reeves & Mangelsd.] into maize ( Z. mays L.), we have generated an interspecific hybrid and its backcross generations (BC 1 F 3 ). The × BC (MZI202) resembled maize, with chromosome number 2 n = 20. MZI202 was studied by multicolor genomic in situ hybridization (McGISH) simple‐sequence repeat (SSR) microsatellites markers. McGISH experiments provided direct evidence that a maize–perennial substitution line...
The coated protein gene of sugarcane mosaic virus ( SCMV CP gene) was cloned from maize Zea mays L.) leaves showing dwarf symptoms by reverse-transcription polymerase chain reaction (RTPCR) with degraded primers. results sequencing and homologous comparison indicated that the strain MDB, 920 bp in length, including an open reading frame (ORF) encoding 219 amino acids a 3' untranslated region (3'UTR). This constructed into expression vector p35SCPrBar antisense orientation, under control...
Abstract Temperature stress restricts plant growth and development. Antifreeze protein (AFP) can improve plants antifreeze ability. In our previous study, the AnAFP gene cloned from Ammopiptanthus nanus was confirmed to be an excellent candidate enhancing cold resistance. But, shared similar structures with KnS type dehydrins including K, N S domains except ice crystal binding domain A. Here, we generated AnAFPΔA , ΔK, AnAFPΔN ΔS, transformed them into ordinary sensitive strains of E. coli...
BES1/BZR1s are plant-specific transcription factors and play crucial roles in plant growth, development response to environmental stress. The Ammopiptanthus nanus (A. nanus) is a relict-xerophyte shrub tolerates various of abiotic stimuli. However, few genes identified from A. nanus. To date, the information function AnBES1/BZR1s remains unknown. In present study, we genome-wide analysed characterized their properties through sequence analysis expression profiling against osmotic total,...
The Pumilio (Pum) RNA-binding protein family regulates post-transcription and plays crucial roles in stress response growth. However, little is known about Pum plants. In this study, a total of 19 ZmPum genes were identified classified into two groups maize. Although each contains the conserved domain, members show diversity gene architectures, physicochemical properties, chromosomal location, collinearity, cis-elements, expression patterns. typical proteins have eight α-helices repeats,...
Maize varieties with high amylose proportion are more valuable for starch industry. The SBEⅡb gene encodes one of the branching isozymes (SBEⅠ, SBEⅡa, and SBEⅡb). Its recessive mutant amylose-extender (ae/sbe2b) decreases total activities SBEs increases up to 60%. Here, breeding potential introduced germplasm line GEMS-0067 was evaluated by genotyping phenotyping. deletion ninth exon gene, proportion, typical irregular granules suggested that this derived from same resource AE11....
Abstract Maize is acutely vulnerable to water deficit. Due the slow progress in conventional breeding, more beneficial genes should be transformed into maize for improvement of drought tolerance. In present study, vacuolar H + ‐pyrophosphatase (H ‐PPase) gene ( AnVP1 ) from Ammopiptanthus nanus was by Agrobacterium ‐mediated calli transformation. After identification integration and ectopic expression PCR amplification, DNA resequencing, reverse transcription PCR, real‐time quantitative...