A5102932552- Drug-Induced Hepatotoxicity and Protection
- Liver Disease Diagnosis and Treatment
- Pharmacogenetics and Drug Metabolism
- Computational Drug Discovery Methods
- Environmental Toxicology and Ecotoxicology
- Alcohol Consumption and Health Effects
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Spectroscopy Techniques in Biomedical and Chemical Research
- Diet, Metabolism, and Disease
- Biotin and Related Studies
- Eicosanoids and Hypertension Pharmacology
- Nanoparticle-Based Drug Delivery
- T-cell and Retrovirus Studies
- Hair Growth and Disorders
- Click Chemistry and Applications
- Microplastics and Plastic Pollution
- Electrochemical sensors and biosensors
- Animal testing and alternatives
- Synthesis of Indole Derivatives
- Chronic Myeloid Leukemia Treatments
- Diverse Scientific Research Studies
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
Korea Institute of Toxicology
2004-2023
Korea University of Science and Technology
2012-2023
Seoul National University Hospital
2019
Seoul National University
2019
Tuskegee University
2009
Korea Research Institute of Chemical Technology
2004-2008
National Veterinary Research and Quarantine Service
2005
Karolinska Institutet
2002-2004
Hokkaido University
2000
The University of Texas Southwestern Medical Center
1997
A number of the Saccharomyces cerevisiae vacuolar protein-sorting (vps) mutants exhibit an altered morphology. Unlike wild-type cells that contain 1-3 large structures, class B vps5 and vps17 mutant 10-20 smaller vacuole-like compartments. To explore role these VPS gene products in vacuole biogenesis, we cloned sequenced VPS5 characterized its protein products. The is predicted to encode a very hydrophilic 675 amino acids shows significant sequence homology with mammalian sorting nexin-1....
Metabolic labeling techniques are powerful tools for cell labeling, tracking and proteomic analysis.However, at present, the effects of metabolic agents on metabolism physiology not known.To address this question, in study, we analyzed cells treated with Ac4ManNAz through microarray analysis analyses membrane channel activity, individual bio-physiological properties, glycolytic flux.According to results, treatment 50 μM led reduction major cellular functions, including energy generation...
The intragastric administration of ethanol as part a lowcarbohydrate diet results in alcohol hepatotoxicity. We aimed to investigate whether comparable liver injury can be achieved by oral intake. Male Sprague-Dawley rats were fed low-carbohydrate diets for 36–42 days either intragastrically or orally. Liver pathology, blood concentration, serum alanine amino transferase (ALT), endotoxin level, hepatic CYP2E1 induction, and cytokine profiles assessed. Both resulted marked steatosis with...
Drug-induced liver injury (DILI) is one of the major reasons for termination drug development. Due to importance predicting DILI in early phases development, diverse silico models have been developed filter out DILI-causing candidates before clinical study. However, no computational achieved sufficient prediction power screening because 1) drugs often cause through reactive metabolites, 2) different outcomes mechanisms, and 3) label on not clearly defined. In this study, we binary...
Benzo( a )pyrene (B P) is polycyclic aromatic hydrocarbon that causes mutations and tumor formation. Zacco platypus sentinel species suitable for monitoring aquatic environments. We studied cytochrome P450 system (CYP system) expression DNA adduct formation in the liver of Z. following waterborne exposure to B P. The results showed both dose time dependency. significant induction levels CYP mRNA protein reached maximums at 2 days 14 days, respectively, hepatosomatic index was maximally...
Abstract Summary Drug-induced liver injury (DILI) is a challenging endpoint in predictive toxicology because of the complex reactive metabolites that cause damage and wide range mechanisms involved development disease. ToxSTAR provides structural similarity-based DILI analysis in-house prediction models predict four subtypes (cholestasis, cirrhosis, hepatitis steatosis) based on drug metabolite molecules. Availability implementation freely available at https://toxstar.kitox.re.kr/....
In the present study, cDNA microarray analyses were performed with mouse chips in order to evaluate similarities and differences gene expression profiles for compounds differing their genotoxic carcinogenic potential. Eight test substances evaluated, two each from four classes of compounds: carcinogens (1,2-dibromoethane glycidol), noncarcinogens (8-hydroxyquinoline emodin), nongenotoxic (methyl carbamate o-nitrotoluene), (D-mannitol 1,2-dichlorobenzene). Quadruplicate hybridization...
Eicosanoids play important roles in inflammation, allergy, fever, and immune responses. In the eicosanoid pathway, cyclooxygenase (COX) catalyzes conversion of arachidonic acid to prostaglandins is a crucial target nonsteroidal anti-inflammatory drugs (NSAIDs). Thus, toxicological studies on pathway are for drug discovery evaluation adverse health outcomes due environmental contaminants. However, experimental models limited owing concerns regarding ethical standards. new alternative...
AnimalsSamples were obtained from slaughtered cattle, and cattle submitted for disease diagnosis to the Pathology division, National Veterinary Research Quarantine Service (NVRQS), Korea.Four lymphoma-suspected samples collected at NVRQS diagnostic laboratory January 1993 June 2004 43 abattoir 2002 2004. Gross observation of lymphomaAll part carcass organs in abdominal thoracic cavities carefully observed presence lymphoma-like nodular lesion.However, superficial lymph node, spinal cord,...
Androgenic alopecia (AGA) is the most common type of hair loss. It likely inherited genetically and promoted by dihydrotestosterone. 5α-reductase has been proven a good target through finasteride use. However, pathogenesis AGA cannot be fully explained based only on dihydrotestosterone levels.To identify similar hairloss inhibition activity RE-ORGA with mode action other than finasteride.We prepared from Korean herb mixtures. We performed MTT assays for cytotoxicity, Cell Counting Kit-8 cell...
Safe endocrine-disrupting alternatives for bisphenol A (BPA) are needed because its adverse health effects have become a public concern. Some analogues (bisphenol F and S) been applied, but their potential is either not negligible or weaker than that of BPA. However, the AP (BPAP), another BPA alternative, has yet fully assessed. Hence, we evaluated thyroid hormone (TH)-disrupting potency BPAP THs essential endocrine hormones. Zebrafish embryos were exposed to (0, 18.2, 43.4, 105.9 μg/L) 120...
Carbon tetrachloride (CCl4) induced rat hepatitis was studied by observing an FTIR spectrum of the liver microsomal or homogenate extract compared with those model compounds. The from healthy control rats showed almost same as a mixture phosphatidylcholine and phosphatidylethanolamine (2:1 weight). Intraperitoneal injection CCl4 decreased absorption intensity due to CH in CCH at 3012 cm−1 extract, it developed new 1,2-diacylglycerol band 1070 extract. An HPLC study added assign...