Festuca pratensis (meadow fescue) as the most frost-tolerant species within Lolium–Festuca complex was used a model for research aimed at identifying cellular components involved in cold acclimation (CA) of forage grasses. The work presented here also comprises first comprehensive proteomic on CA group monocotyledonous which are able to withstand winter conditions. Individual F. plants with contrasting levels frost tolerance, high tolerant (HFT) and low (LFT) plants, were selected...

In light of the growing interest in antimicrobial peptides (AMPs) as potential alternatives to traditional antibiotics, proteomic research has increasingly focused on this area. Addressing significant scientific need, we undertook an initiative review and analyze available databases containing information AMPs. These play a pivotal role foundation for most AMP-related studies, enabling not only identification new compounds, but also deeper understanding their properties therapeutic...

Chloroderivatives of phenoxyacetic acid are a group compounds commonly used as plant protection products. Differences in the molecular structure these related to varying substitution and number chlorine atoms aromatic ring. Different structures may affect activity compounds, their physicochemical properties, well toxicity biological effects. A 6 chemical derived from was tested. The analysed using spectroscopic methods (FTIR, FTRaman, UV-VIS, 1HNMR, 13CNMR) quantum computational (DFT)....

Fir honeydew honey is a uniquely beneficial product which often subjected to adulteration; however, pollen analysis not useful verify this type. Fourteen samples of EU protected designation origin fir gathered directly from apiaries were studied. Standards legal requirements and additional parameters, i.e., specific optical rotation, mineral content, antioxidant activity, tested. Five nectar honeys different varieties used as comparative material. HPTLC SDS-PAGE methods fingerprint the...

Snake venom is a rich source of peptides and proteins with wide range actions. Many the components are currently being tested for their usefulness in treatment many diseases ranging from neurological cardiovascular to cancer. It also important constantly search new properties not yet described. The

Abstract An increasing problem in the field of health protection is emergence drug-resistant and multi-drug-resistant bacterial strains. They cause a number infections, including hospital which currently available antibiotics are unable to fight. Therefore, many studies devoted search for new therapeutic agents with bactericidal bacteriostatic properties. One latest concepts this type substances among toxins produced by venomous animals. In approach, however, special attention paid snake...

Honey is a natural sweetener composed mostly of sugars, but it contains also pollen grains, proteins, free amino acids, and minerals. The amounts proportions these components depend on the honey type bee species. Despite low content protein, they are becoming popular study object, have recently been used as markers authenticity quality honey. Currently, most methods protein isolation from dialysis against distilled water, lyophilization dialysate, or various precipitation protocols. In this...

One of the key problems modern infectious disease medicine is growing number drug-resistant and multi-drug-resistant bacterial strains. For this reason, many studies are devoted to search for highly active antimicrobial substances that could be used in therapy against infections. As it turns out, snake venoms a rich source proteins exert strong antibacterial effect, therefore they have become an interesting research material. We analyzed Naja ashei venom such properties, we found specific...

Drone brood is a little-known bee product which frequently considered as male equivalent of royal jelly and sometimes used its adulterant. The aim the study was to compare chemical composition biological activity both products originated from same apiaries (n = 3) limiting influence genetic environmental factors. Moreover, for drone covered testing three stages larval development (days 7, 11, 14). comparison included mineral (ICP-OES method), protein content profile (SDS-PAGE), testosterone...

Background Intraspecific variations in snake venom composition have been extensively documented, contributing to the diverse clinical effects observed envenomed patients. Understanding these is essential for developing effective snakebite management strategies and targeted antivenom therapies. We aimed comprehensively investigate venoms from three distinct populations of N . mossambica Eswatini, Limpopo, KwaZulu-Natal regions Africa terms their protein reactivity with commercial antivenoms...

Naja ashei is an African spitting cobra species closely related to N. mossambica and nigricollis. It known that the venom of ashei, like other cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, attempt was made determine proteome with use 2-D electrophoresis MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time Flight) mass spectrometry techniques. Our investigation revealed main components analysed 3FTxs...

Many imported honeys distributed on the Polish market compete with local products mainly by lower price, which can correspond to quality and widespread adulteration. The aim of study was compare honey samples (11 blends 5 honeys) based their antioxidant activity (measured DPPH, FRAP, total phenolic content), protein profile obtained native PAGE, soluble content, diastase, acid phosphatase activities identified zymography. These indicators were correlated standard parameters (water, HMF, pH,...

The aim of the study was to use protein and polyphenolic profiles as fingerprints goldenrod honey apply them for verification labeled variety. markers 10 samples were correlated with standard physicochemical parameters biological activity measured in vitro antioxidant, antifungal antibacterial activities. Honey proteins examined regarding soluble protein, diastase SDS-PAGE profile. profile obtained HPTLC antioxidant detected colorimetric methods. antimicrobial effect representative different...

Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity pharmacological potential. Much attention has been paid to the study composition different species also detailed analysis properties individual components. Since peptides are active ingredients in venom, rapidly developing proteomic techniques used analyze them. During such analyses, one routine operations measure protein concentration sample. The aim this was compare five...

Snake venom is a complex mixture of proteins and peptides which in the Viperidae mainly hemotoxic. The diversity these components causes to be an extremely interesting object study. Discovered can used search for new pharmaceuticals primarily treatment diseases cardiovascular system. In order determine protein composition southern copperhead venom, we have high resolution two dimensional electrophoresis MALDI ToF/ToF MS-based identification. We identified 10 groups present phospholipase A2...

In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties elapid venoms. the present study, proteome two red spitting cobra Naja pallida (NPV) Mozambique mossambica (NMV) was characterized using LC-MS/MS approach, against three Candida species established. A complex response venom treatment revealed. NPV NMV, when used at relatively high concentrations, decreased cell...

Abstract Proteins and amino acids are minor components of honey that rarely used for its quality evaluation, although these create biological activity as well can serve overheating detection. The aim the study was to use indicators confirm a declared on label variety. Fifty-eight samples 8 different varieties meeting commercial requirements were used, including 28 local Polish 30 (mainly imported from EU non-EU) honeys. For protein profiling previously polyacrylamide electrophoresis in...

The dynamic development of venomics in recent years has resulted a significant increase publicly available proteomic data. information contained therein is often used for comparisons between different datasets and to draw biological conclusions therefrom. In this article, we aimed show the possible differences that can arise, final results experiment, while using research workflows. We applied two software solutions (PeptideShaker MaxQuant) process data from shotgun LC-MS/MS analysis Naja...

The administration of toxin-specific therapy in snake envenoming is predicated on improved diagnostic techniques capable detecting specific venom toxins. Various serological tests have been used snakebite envenoming. Comparatively, enzyme-linked immunosorbent assay (ELISA) has shown to offer a wider practical application. We report an inhibition ELISA for three-finger toxin (3FTx) proteins venoms African spitting cobras. optimized detected 3FTxs N. ashei (including other Naja sp.) venoms,...

Antivenom immunotherapy is the mainstay of treatment for snakebite envenoming. Most parts world affected by envenoming depend on broad-spectrum polyspecific antivenoms that are known to contain a low content case-specific efficacious immunoglobulins. Thus, advances in toxin-specific antibodies production hold much promise future therapeutic strategies We report anti-3FTxs monoclonal developed against N. ashei venom mice. All three test mAbs (P4G6a, P6D9a, and P6D9b) were found be IgG...

Snake venoms are widely studied in terms of their systemic toxicity and proteolytic, hemotoxic, neurotoxic, cytotoxic activities. However, little is known about snake-venom-mediated effects when used at low, noncytotoxic concentrations. In the current study, two human fibroblast cell lines different origin, namely WI-38 fetal lung fibroblasts BJ foreskin were to investigate snake-venom-induced adaptive response a relatively concentration (0.01 µg/ml). The Indochinese spitting cobra ( Naja...

Changes in proteome level as a result of methyl jasmonate and cholesterol treatment were investigated. The identified proteins often involved response to stress caused by various treatments. Furthermore, 18 expressed treatment-specific manner. In this study, the fenugreek plants treated with (as an elicitor) precursor steroids steroidal saponins) check reaction at investigate saponin (diosgenin) biosynthesis. Proteins separated two-dimensional electrophoresis (2-DE) MALDI-ToF/ToF followed...