Context: The most common biomaterial used for dental implants is titanium. However, the release of metal ions and risk allergic reactions to metals that may occur in some patients cannot be avoided. Hydroxyapatite-polymethylmethacrylate (HA-PMMA) composite biomaterials are proposed have potential as implant due their mechanical, chemical, biological properties. HA-PMMA induce osseointegration, biocompatible, less reactions, no released. In addition, can obtained from Indonesia’s abundant...

Periodontal disease is a chronic inflammation of periodontal tissue as result complex interactions between subgingival pathogenic bacteria and the host immune system. Aggregatibacter actinomycetemcomitans Porphyromonas gingivalis are periodontopathogenic that can increase inflammatory response cause damage. Antibacterial agent needed to inhibit growth so tissues health in periodontitis patients improved. Objective: To determine inhibition spiny amaranth extract (Amaranthus spinosus) on...

Introduction: Periodontopathogenic bacteria are Gram-negative anaerobic that often cause peri-implantitis such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum. Objective: This study is to analyze the periodontopathogen antibacterial properties of Polymethylmethacrylate (PMMA) Hydroxyapatite (HA) composites against growth F. nucleatum, P. A. Actinomycetemcomitans in vitro. Materials Methods: Inhibition zone PMMA-HA periodontopathogenic was...

Modification of implant material can affect osseointegration between the surface and bone. Polymethylmethacrylate (PMMA) has potential as an material, but drawbacks such exothermic properties formation fibrous capsule tissue after implantation. PMMA must be combined with active ingredients like hydroxyapatite (HA) due to its osteoconductive properties. HA obtained from animals (xenograft), limestone (Alloplast). The presence osteogenic markers Runt-related transcription factor 2 (RUNX2)...

Periodontal disease if left untreated may result in tooth loss. Implants are a form of rehabilitative therapy for replacement. However, implants quite expensive Indonesia. Therefore, there is the need an alternative dental implant biomaterial with biocompatible properties that can effectively promote osseointegration. One such material polymethylmethacrylate (PMMA), which widely used by orthopedic surgeons as implants. PMMA lacks bioactivity necessary effective Unlike PMMA, hydroxyapatite...

Traumatic ulcers are one of the most common mucosal diseases. Ulcerated surfaces commonly formed as a result injuries involving soft tissues oral cavity. Injuries can occur events such accidentally biting oneself while speaking or sleeping. The goal this study was to see if there were any variations in IL-6 expression after Wistar rats treated topically with probiotic Lactobacillus casei Shirota treat traumatic ulcers. Research methods24 healthy male weighing 175-250 grams and aged 2-3...

Probiotics are living microorganisms that can provide health advantages. A popular probiotic called Lactobacillus casei affect the immune response by increasing number of macrophages and mast cells involved in healing traumatic ulcers. This study aims to investigate how administering Shirota probiotics locally systemically affects amount plasma throughout Wistar rats' The experiment 36 healthy male rats, weighing between 175-250g aged 2-3 months. labial area inferior incisor arch was...

The blood-brain barrier (BBB) is an essential physiological that regulates the transport of substances from circulation to brain. Accurate prediction BBB permeability for understanding drug delivery brain and developing effective therapies neurological disorders.Clinical experiments have provided a more accurate measure permeability.Nevertheless, these methods take time are labor-intensive.Consequently, several computational attempted predict permeability; however, their accuracy remains...

Post-tooth extraction socket preservation is necessary due to alveolar bone resorptive patterns through regenerative dentistry approaches that involve the use of stem cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth (SHED) are known potentially possess osteogenic ability. Meanwhile, carbonate apatite (CAS) can act as a biocompatible capable supporting mesenchymal (MSCs) proliferate differentiate optimally. The aim this study investigate expression...

Polymethylmethacrylate (PMMA) has been widely used, but it several fallback properties in its interaction with bone tissue, so the addition of hydroxyapatite (HAp) material aims to improve biocompatibility, regeneration process, and osteointegration implants. The HAp can be sourced from bovine processed through Good Manufacturing Practice Tissue Bank (HApGMP), limestone (CaCO3) by Balai Besar Keramik (HApBBK).This study was observe expression vascular endothelial growth factor (VEGF) Bone...

To observe the expression of Runt-Related Transcription Factors 2 (RUNX2) and Alkaline Phosphatase (ALP) markers in osteoblast cell cultures exposed to Polymethylmethacrylate (PMMA) combined with hydroxyapatite (HAp) material improve osteointegration bone implants.Sample PMMA HAp materials a mixture made from limestone as natural source which processed through Balai Besar Keramik (HApBBK) first group bovine Good Manufacturing Practice (HApGMP) second group. Twenty-four fetal rat calvarie...

Tissue engineering using Stem cell from Human Exfoliated Deciduous Teeth (SHED) and a natural biomaterials biomaterial scaffold has become promising therapy for the alveolar bone defect. The aim of this study was to analyze Osteoprotegerin (OPG) Receptor Activator NF-Κb ligand (RANKL) expression after application Hydroxyapatite SHED.A laboratory experimental research with post test-only control group. 14 male Wistar rats weighing 260 280 g were used as animal study. animals randomly assigned...

Background: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite scaffold (CAS) may have multiple functions that could be used to regenerate the alveolar bone defects. The purpose of this study is examine ability SHED and CAS defects using an immunohistochemical analysis. Methods: ten three-month-old healthy male Wistar rats (R. novergicus) weighed between 150-250 grams (g) were as animal models. A simple blind random sampling method was select sample assigned...

Periodontitis progression is characterized by alveolar bone loss, and its prevention a major clinical problem in periodontal disease management. Matrix metalloproteinase-8 (MMP-8) has been shown to adequately monitor the treatment of chronic periodontitis patients as gingival crevicular fluid MMP-8s were positively associated with severity disease. Moreover, modulating vascular endothelial growth factor (VEGF) levels bones could be good way improve regeneration cure VEGF promotes cell...

Objective: To investigate the regeneration of rat’s salivary gland diabetic defect after intraglandular transplantation Human Dental Pulp Stem Cells (HDPSCs) on acinar cell vacuolization and Interleukin-10 (IL-10).  Material Methods: HDPSCs isolated from dental pulp first premolars #34. 3rd passage was characterized by immunocytochemistry CD73, CD90, CD105 CD45. Twenty-four male Wistar rats, 3-month-old, 250-300 grams induced with Streptozotocin 30 mg/kg body weight to create diabetes...

Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance other side effects. Lactobacillus casei Shirota can affect the number fibroblasts blood vessels in wound healing. aim this study was investigate difference fibroblast cells after topical systemic administration L. probiotics Wistar rats with ulcer. Materials Methods: Overall, 36 healthy male aged 2-3 months old weighing 175-250 g body weight were used sample. Traumatic ulcer made on labial...

Background: Bone graft material is used with periodontal flap procedure that expected to help the growth of new bone through process osteogenesis, osteoinduction, and osteoconduction. Some work must be done support regeneration tissue, including three key elements principal, such as scaffolds (collagen material), signal molecules (growth factors) cells. IGF-1 a factor has been studied stimulate replication osteoblasts matrix synthesis remodeling process. Osteocalcin specific product...

<ns4:p><ns4:bold>Background</ns4:bold>: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite scaffold (CAS) may have multiple functions that could be used to regenerate the alveolar bone defects. The purpose of this study is examine ability SHED and CAS defects using an immunohistochemical analysis.</ns4:p><ns4:p> <ns4:bold>Methods</ns4:bold>: ten three-month-old healthy male Wistar rats <ns4:italic>(R. novergicus</ns4:italic>) weighed between 150–250 grams (g)...

Abstract Objectives Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone tissue engineering and therapy regenerative medicine. It been combined with biomaterials such as mixed of polymethylmethacrylate (PMMA) hydroxyapatite (HA) candidates synthetic graft biomaterial. The aim this study was to analyze the toxicity test PMMA-HA scaffold seeded SHED osteoblast in vitro. Materials Methods isolated pulp noncarious cells were cultured, exposed scaffolds three...

Surgery is required to restore bone loss brought on by regenerative periodontal diseases while retaining the patient's aesthetics. The deficits caused disease have been repaired using a variety of transplant materials. One graft materials used dentin since it resembles in terms both organic and inorganic components. In order evaluate viability dental grafts, this study intended count osteoblast cells that were still alive after specific therapy. Osteoblast cell cultures 42 well plates...

The success of implant placement will depend on the ability material to integrate with surrounding tissue. Polymethylmethacrylate (PMMA) has been used as an material, but it several fallback properties in its interaction bone addition hydroxyapatite (HAp) PMMA is expected produce reinforced bioceramic polymers better mechanical and biological properties. purpose this study was evaluate expression osteopontin osteocalcin cultured osteoblasts when exposed two candidate materials: PMMA-HApGMP,...

This study aims to examine the toxicity of collagen extracted from gouramy fish scales (Oshpronemus gouramy) by evaluating its viability against baby hamster kidney fibroblasts-21.Collagen was (O. with 6% acetic acid. Its results were analyzed using Fourier-transform infrared spectroscopy and freeze-dried technique. morphology then scanning electron microscope. Afterward, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay conducted compare cells without scale...