A5102767277- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Antimicrobial agents and applications
- Fermentation and Sensory Analysis
- Advanced Sensor and Control Systems
- Hydraulic and Pneumatic Systems
- Ruminant Nutrition and Digestive Physiology
- Fecal contamination and water quality
- Bacillus and Francisella bacterial research
- Advanced Algorithms and Applications
- Biosensors and Analytical Detection
- Industrial Technology and Control Systems
- Analytical Chemistry and Chromatography
- Animal Disease Management and Epidemiology
- Food Safety and Hygiene
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Manufacturing Process and Optimization
- Proteins in Food Systems
- Microbial Metabolism and Applications
Roman L. Hruska U.S. Meat Animal Research Center
2016-2025
Agricultural Research Service
2013-2024
East China University of Science and Technology
2017-2024
Tianjin University of Technology
2024
Changchun University of Chinese Medicine
2023
North University of China
2022
Institute of Subtropical Agriculture
2022
Zhengzhou University of Light Industry
2021
The University of Tokyo
2020
Northwest A&F University
2019-2020
Spores produced by bacilli are encased in a proteinaceous multilayered coat and, some species (including Bacillus anthracis), further surrounded glycoprotein-containing exosporium. To characterize bacillus spore surface morphology and to identify proteins that direct formation of features, we used atomic-force microscopy (AFM) image the surfaces wild-type mutant spores subtilis, as well cereus 569 Sterne strain anthracis. This analysis revealed these strains populated series bumps ranging...
Bovine peripheral lymph nodes (LNs), including subiliac LNs, have been identified as a potential source of human exposure to Salmonella enterica, when adipose trim containing these is incorporated into ground beef. In order gain better understanding the burden S. enterica in LNs feedlot and cull cattle, cross-sectional study was undertaken which 3327 were collected from cattle at harvest seven plants, located three geographically distinct regions United States. Samples seasons: Fall 2010,...
Specific concerns have been raised that third-generation cephalosporin-resistant (3GC(r)) Escherichia coli, trimethoprim-sulfamethoxazole-resistant (COT(r)) E. 3GC(r) Salmonella enterica, and nalidixic acid-resistant (NAL(r)) S. enterica may be present in cattle production environments, persist through beef processing, contaminate final products. The prevalences concentrations of these organisms were determined feces hides (at feedlot processing plant), pre-evisceration carcasses, carcasses...
To more fully characterize the burden of Salmonella enterica in bovine peripheral lymph nodes (PLN), PLN (n = 5,450) were collected from healthy cattle at slaughter 12 commercial abattoirs that slaughtered feedlot-fattened (FF) exclusively 7), removed (or culled) breeding herds 3), or both FF and cull 2). Qualitative quantitative methods used to estimate prevalence concentration PLN. Isolates subjected a variety phenotypic, serological, molecular assays. Overall, was 7.1 1.8%. However,...
Salmonella enterica is a leading cause of foodborne illness in the U.S. In meat industry, one action taken to address pathogen contamination incidence an intense sanitization (IS) entire processing plant that many large processors perform annually or semiannually. However, this procedure’s immediate and long-term impact on environment microbial community colonization are unknown. Here we investigated IS procedure environmental biofilms subsequent S. stress tolerance. Environmental samples...
Biofilm formation by foodborne pathogens is a serious threat to food safety and public health. Meat processing plants may harbor various microorganisms occasional pathogens; thus, the environmental microbial community might impact pathogen survival via mixed biofilm formation. We collected floor drain samples from two beef with different E. coli O157:H7 prevalence history investigated effects of on sanitizer tolerance. The results showed that forming ability bacterial species composition...
Salmonella enterica represents a diverse group of pathogens commonly associated with food contamination including red meat. Even though pre- and post-harvest cleaning sanitization procedures are widely implemented at meat processing plants to mitigate the hazard, S. cells may escape process by colonizing, on contact, surfaces in form biofilm that functions as an aggregated microbial community facilitate mutual protection, antimicrobial resistance, proliferation dissemination. Biofilm...
In the beef industry, product contamination by Salmonella enterica is a serious public health concern, which may result in human infection and cause significant financial loss due to recalls. Currently, precise mechanism pathogen source responsible for commercial establishments are not well understood. We characterized 89 S. strains isolated from trim with respect their biofilm-forming ability, antimicrobial resistance, biofilm cell survival/recovery growth after sanitizer exposure. A total...
The lipopolysaccharides of Actinobacillus actinomycetemcomitans strain Y4 and a human clinical isolate PO 1021-7 were examined by SDS/PAGE, deoxycholate/PAGE mass spectrometry. PAGE analysis revealed an electrophoretic pattern similar to the SR-type lipopolysaccharide (LPS) Salmonella. Deoxycholate/PAGE indicated LPS A. consist short sugar chains. Chemical presence thiobarbituric-acid-positive material (3-deoxy-D-manno-octulosonic acid equivalents) four neutral sugars: glucose, galactose,...
Two new sacchariferous starters were developed based on functional strains isolated from the pits of several famous Chinese Luzhou-flavor liquor brewers and a genetically engineered mold strain previously constructed in this laboratory. Compared with traditional Daqu, starters, especially strain, possessed higher abilities saccharification fermentation as well alcohol yield ratio percent conversion. Applying these production has advantages that include easy preparation, convenient operation...
The genome of Thermoanaerobacter tengcongensis is estimated to encode 2588 theoretical proteins. In this study, we have vitalized approximately 46% the proteome experimentally using a proteomic strategy that combines three different methods, shotgun digestion plus high-performance liquid chromatography (HPLC) with ion-trap tandem mass spectrometry (shotgun-liquid (LC)/MS), one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) HPLC (one-dimensional (1DE)-LC/MS),...
Salmonella enterica serovar Montevideo has been linked to recent foodborne illness outbreaks resulting from contamination of products such as fruits, vegetables, seeds and spices. Studies have shown that also is frequently associated with healthy cattle can be isolated ground beef, yet human salmonellosis beef are rare. This disparity fuelled our interest in characterizing the genomic differences between strains products, those patients outbreak sources. To end, we sequenced 13 completion,...