A5034969012- Advanced biosensing and bioanalysis techniques
- Molecular Sensors and Ion Detection
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Luminescence and Fluorescent Materials
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Ginger and Zingiberaceae research
- Mass Spectrometry Techniques and Applications
- Cytokine Signaling Pathways and Interactions
- Molecular Junctions and Nanostructures
- Carbon dioxide utilization in catalysis
- Advanced Proteomics Techniques and Applications
- Graphene research and applications
- Nanomaterials for catalytic reactions
- Immune Response and Inflammation
- Catalytic Processes in Materials Science
- DNA and Nucleic Acid Chemistry
- Diatoms and Algae Research
- Polymer composites and self-healing
- Berberine and alkaloids research
- Mesoporous Materials and Catalysis
- Cancer-related molecular mechanisms research
- Hedgehog Signaling Pathway Studies
- Plant Growth Enhancement Techniques
Jeju National University
2020-2023
Korea Institute of Industrial Technology
2016-2022
Korea University of Science and Technology
2020
University of Wisconsin–Madison
2018
Government of the Republic of Korea
2016
Agency for Defense Development
2015-2016
Soon Chun Hyang University Cheonan Hospital
2016
Seoul National University
2006-2012
University of Florida
2010
The need to decipher various biological events has led the elucidation of molecular mechanisms underlying a number disease processes. Consequently, detection and simultaneous monitoring chemical interactions between targets become indispensable in medical diagnosis, targeted therapeutics, biology. Multiplexed applications employing nanomaterials, which represent integration nanotechnology biology, have changed bioanalytical outlook provided promising tools. Among these fluorescent dye-doped...
Hormone-specific anticancer drugs for breast cancer treatment can cause serious side effects. Thus, with natural compounds has been considered a better approach as this minimizes effects and multiple targets. 6-Gingerol is an active polyphenol in ginger various modalities, including activity, although its mechanism of action remains unknown. Increases the level reactive oxygen species (ROS) lead to DNA damage induction response (DDR) mechanism, leading cell cycle arrest apoptosis tumorsphere...
Recently, natural compounds have been used globally for cancer treatment studies. Silibinin is a compound extracted from Silybum marianum (milk thistle), which has suggested as an anticancer drug through various Studies on its activity in cancers are undergoing. This study demonstrated the molecular signaling behind of silibinin non-small cell lung (NSCLC). Quantitative real-time polymerase chain reaction and Western blotting analysis were performed analysis. Wound healing assay, invasion...
Recently, significant advances have been made independently in electrogenerated chemiluminescence (ECL) analysis and supramolecular anion sensing. Herein, we demonstrate a new proof of concept for ECL-based pyrophosphate (PPi) sensing, where the emission intensity is changed by electrochemical turn-on. The ECL PPi sensor (1-2Zn) consists two orthogonally bonded moieties: boron dipyrromethene (ECL reporter) phenoxo-bridged bis(Zn2+−dipicolylamine) complex (PPi receptor). presence confirmed...
Although rolling circle amplification (RCA) is an efficient method to produce DNA materials for biomedical applications, it does not yield nano-sized products suitable intracellular delivery. We here provide the ways control size of RCA and show a potential application size-controlled nanoparticles.
Janus kinase 2 (JAK2) and STAT3 signaling is considered a major pathway in lipopolysaccharide (LPS)‑induced inflammation. Toll‑like receptor 4 (TLR‑4) an inflammatory response that activates JAK2 during transcription factor for the pro‑inflammatory cytokine IL‑6 Sulfur essential element amino acids required growth development. Non‑toxic sulfur (NTS) can be used livestock feeds as it lacks toxicity. The present study aimed to inhibit LPS‑induced inflammation C2C12 myoblasts using NTS by...
The detecting and screening of explosives has become a global consideration in dealing with potential terrorism threats the misuse high explosives. Several detection techniques have been developed, but their disadvantages include requirement expensive equipment, complicated pre-treatment, prolonged testing. So far, fluorescence-based sensors great interest as they overcome limitations other techniques. BODIPY, which is fluorophore, shows excellent fluorescence features, it can be used sensor...
This study describes the development and characterization of a novel dendritic signal amplification strategy. It relies on use two different Ru(bpy)32+-doped silica nanoparticles (Probe1,2RSNP Probe2cRSNP) coated with complementary DNAs, which can be simply conveniently self-assembled to build sandwich-type architectures gold grid. The performance this route was demonstrated in conjunction electrogenerated chemiluminescent (ECL) detection target DNA. Compared normal amplification, allowed...
Iron homeostasis is considered a key factor in human metabolism, and abrogation the system could create adverse effects, including cancer. Moreover, 6-gingerol widely used bioactive phenolic compound with anticancer activity, studies on its exact mechanisms non-small cell lung cancer (NSCLC) cells are still undergoing. This study aimed to find mechanism of death induction by NSCLC cells. Western blotting, real-time polymerase chain reaction, flow cytometry were for molecular signaling...
A great deal of effort has been made to develop devices and methods for detecting toxic heavy metals their compounds. Traditionally, such as mass spectrometry atomic absorption have used the detection these metal ions. However, in spite superb sensitivity, equipment can be operated only by experts requires pre-treatment steps taking more than 10 hours. On other hand, spectrophoto-metric often determination ions, due advantages simplicity inexpensive instrumentation. they cannot practice...
High glucose-induced inflammation leads to atherosclerosis, which is considered a major cause of death in type 1 and 2 diabetic patients. Nuclear factor-kappa B (NF-κB) plays central role high activated through toll-like receptors (TLRs) as well canonical protein kinase C-dependent (PKC) pathways. Non-toxic sulfur (NTS) methylsulfonylmethane (MSM) are two sulfur-containing natural compounds that can induce anti-inflammation. Using Western blotting, real-time polymerase chain reaction, flow...
The selective isolation of phosphorylated peptides and subsequent analysis using mass spectrometry is important for understanding how protein kinase phosphatase signals can precisely modulate the on/off states signal transduction pathways. However, detection multi-phosphorylated still limited due to their distinct affinity various materials poor ionization efficiency. Here, we report a highly efficient enrichment binuclear Zn2+-dipicolylamine complex-coated magnetic microspheres (ZnMMs)....
Easy detection: The target DNA in a 10–100 aM range can be detected by pre-complexed nanoparticles without additional amplification or labeling. [Ru(bpy)3]2+-doped silica are hybridized to form complex with highly enhanced sensitivity (see scheme). This method will significant improvement over conventional microarray/fluorescence readout systems. Detailed facts of importance specialist readers published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited typeset....
Abstract DNA aptamers are integrated into synthetic hydrogel networks with the aim of creating hydrogels that undergo volume changes when exposed to target molecules. Specifically, single‐stranded in cDNA‐bound, extended state incorporated as cross‐links, so nanoscale conformational change upon binding molecules will induce macroscopic decreases hydrogels. Hydrogels incorporating adenosine triphosphate (ATP)–binding controllable up 40.3 ± 4.6% ATP, depending on concentration network,...
Lipopolysaccharide (LPS)-induced inflammatory response leads to serious damage, up and including tumorigenesis. Natural mineral sulfur, non-toxic sulfur (NTS), methylsulfonylmethane (MSM) have anti-inflammatory activity that may inhibit LPS-induced inflammation. We hypothesized compounds could responses in CCD-986Sk skin fibroblasts. used Western blotting real-time PCR analyze molecular signaling treated untreated cultures. also flow cytometry for cell surface receptor analysis, comet assays...
Embryonic cancer cells (CSCs) could cause different types of cancer, a skill that makes them even more dangerous than other cells. Identifying CSCs using natural products is good option as it inhibits the recurrence with moderate various effects. Ursolic acid (UA) pentacyclic triterpenoid extracted from fruit and herbal remedies has known anticancer functions against However, its potential remains uncertain. This study was planned to examine induction cell apoptosis by UA. For signaling...
A synthetic fluorescent sensor (sensor 1) is developed using boron-dipyrromethene as a fluorophore and phenoxo-bridged dipicolylamine receptor. Sensor 1 shows significant enhancement of intensity high selectivity for Zn2+ over various metal cations. The electrochemical study provides rationale the fluorescence turn-on upon complexation with by blocking photo-induced electron transfer mechanism. can be used to image in A549 cells.
For the fabrication of a peelable coating material that decomposes methyl paraoxone (MPO), nerve agent simulant, self-crosslinkable waterborne polyurethanes (PUs) containing silane groups at ends and metal organic framework (UiO-66) were synthesized. UiO-66 dispersed PU solutions for spray prepared by controlling amount in content UiO-66. PUs with large (more than 7.2 wt.%) easily gelated adding because solution was changed from neutral (pH = 7.3) to strongly acidic 2.5). Therefore, should...