A5052519811- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Insect-Plant Interactions and Control
- Entomopathogenic Microorganisms in Pest Control
- Studies on Chitinases and Chitosanases
- Insect Pest Control Strategies
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Plant Virus Research Studies
- Insect Utilization and Effects
- Invertebrate Immune Response Mechanisms
- Insect behavior and control techniques
- Plant and animal studies
- Biological Control of Invasive Species
- Hemiptera Insect Studies
- Plant Parasitism and Resistance
- Virus-based gene therapy research
- Forest Insect Ecology and Management
- Orthoptera Research and Taxonomy
- Viral Infections and Vectors
- Insect and Arachnid Ecology and Behavior
- Nematode management and characterization studies
- Protein purification and stability
- Bacteriophages and microbial interactions
- Genomics, phytochemicals, and oxidative stress
Saskatoon Research and Development Centre
2004-2024
Agriculture and Agri-Food Canada
2013-2024
BC Research (Canada)
2002-2024
University of Saskatchewan
2006-2021
University of Wisconsin–La Crosse
2014
University of Alberta
1995
Queen's University
1983-1985
Abstract: The polymerase chain reaction (PCR) revolutionized the field of diagnostics, and today it has routine applications in medical, veterinary, forensic botanical sciences. fields biological control insect pest management have generally been slow to adopt PCR‐based diagnostics comparison with other science. However, there increasing interest use molecular diagnostic tools arthropod control. In applied entomology, techniques used for identification systematics; however, are increasingly...
Baculovirus occlusion-derived virus (ODV) initiates infection of lepidopteran larval hosts by binding to the midgut epithelia, which is mediated per os infectivity factors (PIFs). Autographa californica multiple nucleopolyhedrovirus (AcMNPV) encodes seven PIF proteins, PIF1 PIF4 form a core complex in ODV envelopes PIF0 and PIF6 loosely associate. Deletion any pif gene results being unable bind or enter cells. AC83 also associates with complex, this study further analyzed its role oral...
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac96 is a core gene, but its role in virus replication still unknown. To determine the baculovirus life cycle, we used AcMNPV bacmid system to generate an ac96-null (vAc(96)(null)). Our analyses showed that absence of does not affect budded (BV) production or viral DNA infected Sf9 cells. Western blotting and confocal immunofluorescence analysis AC96 expressed both cytoplasm nucleus throughout infection. In addition, was detected...
Abstract Biopesticides have the potential to play an important role in sustainable, environmentally safe pest control. A diverse range of biopesticides employ chemical modes action. This review explores three such biopesticides: a fungus used weed control, beneficial bacteria controlling fungal and bacterial disease virus active against insect pests. Through these case studies, we demonstrate that rely on both biochemical approaches complementary biological modalities. Hence, are more...
Protection from parasitism by a virus Parasitoid wasps have developed myriad systems to overcome the defense mechanisms of their hosts as they lay eggs in bodies and targeted species. Gasmi et al . report how host can fight back when infected that expresses protein conferring resistance parasitoid. When members butterfly moth family are wasps, has evolved is horizontally carried viruses—and sometimes incorporated into genome—and impairs ability parasitoid offspring fully develop emerge....
ABSTRACT The cry gene content of Bacillus thuringiensis subsp. aizawai HD-133 was analyzed by a combination high-pressure liquid chromatography (HPLC) and exclusive PCR. A total six genes were detected in genomic DNA purified from HD-133, four the cry1 family ( cry1Aa , cry1Ab cry1C cry1D ) as well each cry2 cry2B cry1I families. To directly determine which expressed crystallized parasporal inclusions, solubilized trypsinized crystals subjected to chromatographic separation HPLC. Only three...
Baculovirus occlusion-derived virus (ODV) infects insect midgut cells under alkaline conditions, a process mediated by highly conserved per os infectivity factors (PIFs), P74 (PIF0), PIF1, PIF2, PIF3, PIF4, and PIF5 (ODV-E56). Previously, multimolecular complex composed of was identified which proposed to play an essential role during ODV entry. Recently, more proteins have been that important roles in oral infectivity, including PIF5, SF58, might work concert with previously known PIFs...
Abstract The activities of digestive protease within the midgut Mamestra configurata (bertha armyworm) larvae were examined using specific substrates and inhibitors. bulk activity was associated with serine proteases comprising trypsin‐, chymotrypsin‐, elastase‐like enzymes. At least 10–15 isozymes detected one‐dimension gelatin gel electrophoresis. Cysteine or aspartic not present; however, amino‐ carboxypeptidase extract. Midgut active in pH range 5.0–12.0 peaks at 7.5 11.0. In general,...
Summary Accurate identification of natural enemies is the cornerstone biological control, and methods that can separate closely related species are essential in ecological studies parasitoids. Conventionally, host rearing dissection used to define range candidate control agents assess host‐specificity However, molecular may be more suitable for evaluation host–parasitoid associations. To demonstrate utility diagnostics host‐range on parasitoids Lygus plant bugs, rearing, multiplex polymerase...
Abstract One‐ and two‐dimensional gel electrophoresis coupled with liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) three insect intestinal lipases (McIIL1, McIIL2 McIIL3) associated the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 active activities were detected in midgut. McIIL genes expressed exclusively midgut; however, all larval stages, whereas McIIL1 mainly feeding larvae McIIL3...
Autographa californica multiple nucleopolyhedrovirus ac68 is a core gene that overlaps lef3 which encodes the single-stranded DNA binding protein. A knockout (KO) virus lacking both and was generated (lef3-ac68 2×KO) to enable functional study of ac68. To produce an ac68KO did not impact expression, lef3-ac68 2×KO repaired with fragment containing ac68, in contained point mutations so only LEF3 expressed. Repair just lef3KO virus. Analysis showed viral replication budded (BV) levels were...