In this work, direct electron transfer (DET)-type extended gate field effect transistor (EGFET) enzymatic sensors were developed by employing DET-type or quasi-DET-type enzymes to detect glucose lactate in both 100 mM potassium phosphate buffer and artificial sweat. The system employed either a dehydrogenase oxidase, the latter of which was mutant enzyme with suppressed oxidase activity modified amine-reactive phenazine ethosulfate. These immobilized on electrodes. Changes measured drain current (

Glutamate and dopamine (DA) represent two key contributors to striatal functioning, a region of the brain that is essential motor coordination motivated behavior. While electroanalytical techniques can be utilized for rapid, spatially resolved detection DA in interferent-rich environment, glutamate, nonelectroactive analyte, cannot directly detected using techniques. However, it probed enzyme-based sensors, which generate an electroactive reporter presence glutamate. The vast majority...

Carbon nanotubes (CNTs) are known for their excellent conductive properties. Here, we present two novel methods, "sandwich" (sCNT) and dual deposition (DD CNT), incorporating CNTs into electrospun polycaprolactone (PCL) gelatin scaffolds to increase conductance. Based on CNT percentage, the DD contain significantly higher quantities of than sCNT scaffolds. The inclusion increased electrical conductance from 0.0 ± 0.00 kS (non-CNT) 0.54 0.10 5.22 0.49 CNT) when measured parallel arrays 0.25...

Abstract Recyclable and biodegradable microelectronics, i.e., “green” electronics, are emerging as a viable solution to the global challenge of electronic waste. Specifically, flexible circuit boards represent prime target for materials development increasing utility green electronics in biomedical applications. Circuit board substrates packaging good dielectrics, mechanically thermally robust, compatible with microfabrication processes. Poly(octamethylene maleate (anhydride) citrate)...

Knee osteoarthritis is a major cause of global disability and cost for the healthcare system. Lower extremity loading determinant knee onset progression; however, technology that assists rehabilitative clinicians in optimizing key metrics lower significantly limited. The peak vertical component ground reaction force (vGRF) first 50% stance highly associated with biological patient-reported outcomes linked to symptoms. Monitoring maintaining typical vGRF profiles may support healthy gait...

Introduction In this paper, we aim to utilize an extended gate field-effect transistor (EGFET)-based transducer measure insulin. The design of EGFETs is characterized by a unique structural arrangement, where physical barrier exists between the semiconductor component and analyte or medium. Due structure, EGFET possesses advantage protecting electrode from intricacies within measurement solution need for redox probe also effectively eliminated incorporating EGFETs, marking significant...

Most affinity-based biosensors are designed to be single-use devices, based on the measurement of irreversible binding events, which makes longitudinal monitoring resource-intensive, and typically prohibits analyte fluctuations over time using same device. Selective reversal biorecognition i.e., regeneration, may enable repeated use biosensors; however, typical regeneration methods utilize additional chemical reagents, requiring longer processing times increasing likelihood operator error....

Textile electronics is a rapidly evolving industry, particularly in sectors of wellness, medical, and athletics. The advantage textile electronic, over current wrist worn wearables products, the ability to form non-noticeable integrated system multiple sensors across large contact area that garment has intimate with body. Key challenges exist commercialization these technologies, as it relates identifying consumer needs for justifying use technology cost factors fabrication are limited by...

Abstract Recyclable and biodegradable microelectronics, i.e., “green” electronics, are emerging as a viable solution to the global challenge of electronic waste. Accordingly, development novel materials replace passive components packaging is necessary realize sustainable manufacturing growing distribution devices. Specifically, alternatives printed circuit boards (PCBs) represent prime target for increasing utility green electronics in biomedical Internet-of-Things (IoT) applications. Ideal...

Monitoring of plant growth within agriculture is essential for ensuring the survival crops and optimization resources in face environmental industrial challenges. Herein, we describe a low-cost easily deployable flexible circuit board sensor measurement stalk growth, providing remote tracking development on an scale. Three topologies strategies - "ladder-type," "multiplex-type," "mixed-type" are initially assessed off-plant simulated experiment. Further "multiplex-type" on-plant validation...

Heart disease is the leading cause of death in U.S. and worldwide. Cardiac tissue engineering offers promising diagnostic tools treatments. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are an attractive cell source for these applications due to their patient-specificity theoretical capacity supply limitless quantities. However, hiPSC-CMs immature compared healthy, adult cardiomyocytes, demonstrating morphology, metabolics, electrophysiology. Previous research has...

Abstract Knee osteoarthritis is a major cause of global disability and cost for the healthcare system. Lower extremity loading determinant knee onset progression; however, technology that assists rehabilitative clinicians in optimizing key metrics lower significantly limited. The peak vertical component ground reaction force (vGRF) first 50% stance highly associated with biological patient-reported outcomes linked to symptoms. Monitoring maintaining typical vGRF profiles may support healthy...

Figure 1. Scaffold (i) and bioreactor (ii) viability for iPSC-CM conduction-stimulation platform.Heart disease is the leading cause of death worldwide. Cardiac tissue engineering offers various solutions, frequently employing induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). iPSC-CMs can be expanded limitlessly used autologously; however, they are immature compared to healthy, adult in vivo . We interested conductive scaffolds mature by facilitating gap-junctional coupling...

Conformal electrodes provide a soft and conforming interface with the skin for reduced impedance, comfortable contact, improved signal quality compared to commercial electrodes. In this paper, we present conformal micropatterned organic-metal (CMOM) our investigation on effect of perforation micropatterning PEDOT:PSS coating. CMOM were characterized then evaluated in vivo against commercial-off-the-shelf was found reduce overall impedance each electrode variant, resulting >97% decrease at...

Wearable inertial sensors represent an opportunity to enable gait monitoring and feedback-based rehabilitation in real-world environments. Here, we describe the development of inexpensive I MU-based wireless body area network capable recording 9-axis motion data from 8 sites on simultaneously. This system can generate comparable existing commercial sensor networks distinguish varying loading conditions observed during real-time biofeedback-based human subject testing.