A5102709346- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- interferon and immune responses
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Hepatitis C virus research
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Viral gastroenteritis research and epidemiology
- Ubiquitin and proteasome pathways
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Phytochemistry and Biological Activities
- Click Chemistry and Applications
- Coal Properties and Utilization
- Genomics and Chromatin Dynamics
- Cellular transport and secretion
- Microbial Natural Products and Biosynthesis
- Virology and Viral Diseases
- Head and Neck Anomalies
- Bee Products Chemical Analysis
- Biochemical and Molecular Research
Shaanxi Normal University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Huazhong University of Science and Technology
2015
Beijing Union University
2015
Nanjing Agricultural University
2013
University of Chinese Academy of Sciences
2011
Institute of Biophysics
2011
Chinese Academy of Sciences
2011
Long noncoding RNAs (lncRNAs) participate in host antiviral defense by modulating immune responses. However, it remains largely unexplored how viruses exploit interferon (IFN)-independent lncRNAs to facilitate viral replication. Here, we have identified a group of human that modulate influenza A virus (IAV) replication loss-of-function screen and found an IFN-independent lncRNA, called IPAN, is hijacked IAV assist IPAN specifically induced infection independently IFN associates with...
Mannosylerythritol lipid-A (MEL-A) is a kind of novel biosurfactant and has great potential to apply into food pharmaceutical field with its outstanding physicochemical biological property. In this study, Thyme essential oil (TEO) microcapsules based on MEL-A were prepared through ultrasonic emulsification characterized by size, morphology, structure, antioxidant antibacterial activity. The results showed the optimal preparation condition was duration 15 min power intensities 400 W/cm2...
Mounting evidence indicates that Zika virus (ZIKV) is closely related to neurological disorders such as microcephaly and Guillain-Barré syndrome. There are currently no effective vaccines FDA-approved inhibitors against ZIKV infection. The flaviviral heterodimeric serine protease NS2B-NS3 plays an essential role in maturation replication, thus becoming a promising target anti-ZIKV therapy. Herein, we developed fluorescence-based screening assay search for targeting the (ZIKVpro), identified...
Abstract Schlafen-5 (SLFN5) is an interferon-induced protein of the Schlafen family, which are involved in immune responses and oncogenesis. To date, little known regarding its anti-HIV-1 function. Here, authors report that overexpression SLFN5 inhibits HIV-1 replication reduces viral mRNA levels, whereas depletion endogenous promotes replication. Moreover, they show markedly decreases transcriptional activity long terminal repeat (LTR) via binding to two sequences U5-R region, consequently...
Abstract The coronavirus disease 2019 (COVID-19) pandemic has devastated global health. Identifying key host factors essential for SARS-CoV-2 RNA replication is expected to unravel cellular targets the development of broad-spectrum antiviral drugs which have been quested preparedness future viral outbreaks. Here, we identified proteins that associate with nonstructural protein 12 (nsp12), RNA-dependent polymerase (RdRp) using a mass spectrometry (MS)-based proteomic approach. Among candidate...
Human BST-2 inhibits HIV-1 replication by tethering nascent virions to the cell surface. codes Vpu that counteracts down-regulating this restriction factor from This important function makes a potential therapeutic target. Yet, no agents have been reported block antagonizing BST-2. In study, we report small molecule compound IMB-LA abrogates of and thereby strongly suppresses sensitizing virus restriction. Further studies revealed specifically Vpu-mediated degradation restores expression at...
While Highly Active Antiretroviral Therapy (HAART) has significantly decreased the mortality of human immunodeficiency virus (HIV)-infected patients, emerging drug resistance to approved HIV-1 integrase inhibitors highlights need develop new antivirals with novel mechanisms action. In this study, we screened a library microbial natural compounds from endophytic fungus Colletotrichum sp. and identified alternariol 5-O-methyl ether (AME) as compound that inhibits pre-integration steps. Time-of...
Despite that most HIV-infected individuals experience progressive CD4+ T cell loss and develop AIDS, a minority of remain asymptomatic maintain high level counts several years after seroconversion. Efforts have been made to understand the determinants nonprogressive status, exemplified by clinical course elite controllers (ECs) who an undetectable viremia viremic nonprogressors (VNPs) normal count in spite circulating viral load. However, intrinsic mechanism underlying nonprogression...
BST-2 is an interferon-induced host restriction factor that inhibits the release of diverse mammalian enveloped viruses from infected cells by physically trapping newly formed virions onto cell surface. Human Immunodeficiency Virus-1 (HIV-1) encodes accessory protein Vpu antagonizes down-regulating surface.Using a cell-based ELISA screening system, we have discovered lead compound, 2-thio-6-azauridine, restores surface level in presence Vpu. This compound has no effect on expression and Vpu,...
Bone marrow stromal cell antigen 2 (BST-2) inhibits the release of HIV-1 and other enveloped viruses from surface. Vpu binds to BST-2 through an interaction between transmembrane domains (TMD) two proteins induces downregulation surface BST-2, thereby counteracting its antiviral activity. In this study, we designed prepared a modified peptide BST2-TM-P1, which include sequence TMD, keeping property competing with bind Vpu. Biological assay results indicate BST2-TM-P1 could increase level at...
The rapid emergence of highly transmissible SARS-CoV-2 variants poses serious threat to the efficacy vaccines and neutralizing antibodies. Thus, there is an urgent need develop new effective inhibitors against future outbreaks. Here, we have identified a series glycopeptide antibiotics teicoplanin derivatives that bind spike (S) protein, interrupt its interaction with ACE2 receptor selectively inhibit viral entry mediated by S protein. Computation modeling predicts these compounds interact...
Human APOBEC3G (hA3G) is a cytidine deaminase which inhibits HIV-1 replication. The accessory protein viral infectivity factor (Vif) counteracts with hA3G by targeting it for proteasomal degradation. In this work, we constructed and optimized molecular models of the dimer hA3G-Vif complex. modeling study revealed that loop7 motif appears on interfaces both complex dimer. Biochemical analysis provided evidence suggesting binding Vif to results in steric blocking dimerization, implying...
Given the emerging pivotal roles of stimulator interferon genes (STING) in host pathogen defense and immune-oncology, STING is regarded as a promising target for drug development. Cyclic dinucleotides (CDNs) are first-generation agonists. However, their poor metabolic stability membrane permeability limits therapeutic application. In contrast, small-molecule agonists show superior properties such molecular weight, polar character, delivery diversity. The quest potent small-molecular agonist...
APOBEC3G(A3G) is a host cytidine deaminase that incorporates into HIV-1 virions and efficiently inhibits viral replication. The virally encoded protein Vif binds to A3G induces its degradation, thereby counteracting the antiviral activity of A3G. Vif-mediated degradation clearly represents potential target for anti-HIV drug development. Currently, there an urgent need understanding three dimensional structure full-length In this work, we use homology modeling approach propose based on...