A5076040046- Antioxidant Activity and Oxidative Stress
- Free Radicals and Antioxidants
- Atherosclerosis and Cardiovascular Diseases
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Peroxisome Proliferator-Activated Receptors
- Vitamin C and Antioxidants Research
- Cholesterol and Lipid Metabolism
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Phytochemicals and Antioxidant Activities
- Gut microbiota and health
- Cancer, Lipids, and Metabolism
- Nitric Oxide and Endothelin Effects
- Enzyme Production and Characterization
- Endoplasmic Reticulum Stress and Disease
- Lipoproteins and Cardiovascular Health
- Circadian rhythm and melatonin
- Muscle metabolism and nutrition
- Biofuel production and bioconversion
- Trace Elements in Health
- Paraoxonase enzyme and polymorphisms
- Computational Drug Discovery Methods
- Alcohol Consumption and Health Effects
- Enzyme Structure and Function
Medical University of Graz
2013-2024
University of Graz
1997-2009
Austrian Academy of Sciences
1999-2008
Mylan (Switzerland)
2007
Center for Clinical Research (United States)
2007
Université Paris-Est Créteil
2007
Taipei Institute of Pathology
2007
Institute for Solid State Physics and Optics
2003
Institute for Atomic Energy Research
2003
Institute of Biophysics
2003
Molecular profiling from liquid biopsy, in particular cell-free DNA (cfDNA), represents an attractive alternative to tissue biopsies for the detection of actionable targets and tumor monitoring. In addition PCR-based assays, Next Generation Sequencing (NGS)-based cfDNA assays are now commercially available being increasingly adopted clinical practice. However, validity these products as well utility management patients with cancer has yet be proven. Within framework Innovative Medicines...
The highly contagious SARS-CoV-2 virus is primarily transmitted through respiratory droplets, aerosols, and contaminated surfaces. In addition to antiviral drugs, the decontamination of surfaces personal protective equipment (PPE) crucial mitigate spread infection. Conventional approaches, including ultraviolet radiation, vaporized hydrogen peroxide, heat liquid chemicals, can damage materials or lack comprehensive, effective disinfection. Consequently, alternative material-compatible...
Uric acid and ascorbic are important low molecular weight antioxidants in plasma. Their interactions combined effect on Cu 2+ ‐catalysed oxidation of human density lipoprotein were studied vitro. It was found that uric alone becomes strongly prooxidant whenever it is added to shortly after the start (conditional prooxidant). Ascorbic acid, which present plasma at much lower concentrations (20–60 μM) than urate (300–400 μM), itself not a conditional prooxidant. Moreover, ascorbate prevents...
Spray-dried elderberry juice, containing high amounts of anthocyanin glucosides, was investigated for its antioxidant and prooxidant potentials mechanisms. A strong, concentration-dependent prolongation the lag phase copper-induced oxidation human low-density lipoprotein (LDL) found, but maximum rate unchanged. Peroxyl-radical-driven LDL showed both time reduction rate. In case copper-mediated oxidation, anthocyanins were able to reduce α-tocopheroxyl radical α-tocopherol. Clear activity in...
The antioxidant activity of melatonin (MEL) has been considered to constitute part its physiological as well pharmacological effects. However, described herein we found a profound prooxidant micro‐ millimolar concentrations MEL in the human leukemic Jurkat cell line. This effect was increased glutathione‐depleted cells and counteracted by antioxidants. As consequence promoted fas‐induced death. These data therefore indicate that may be modulator cellular redox status, but does not...
Liquid biopsies are emerging as valuable clinical biomarkers for cancer monitoring. Although International Organization Standards (ISO) and Technical Specifications from the European Committee Standardization (CEN/TS) standardized workflows exist, their implementation in practice is underdeveloped. We aimed to assess applicability of ISO CEN/TS standards a real-world setting, with particular focus on evaluating impact preanalytical parameters hemolysis liquid biopsy analysis.
The development of metabolomics in clinical applications has been limited by the lack validation large multicenter studies. Large population cohorts and their biobanks are a valuable resource for acquiring insights into molecular disease mechanisms. Nevertheless, most collections not tailored have created without specific attention to pre-analytical requirements high-quality metabolome assessment. Thus, comparing samples obtained different procedures remains major challenge. Here, 1H...
Both melatonin and its precursor N ‐acetylserotonin have been reported to exert antioxidant properties both in vitro vivo. Since little is known about their activity lymphocytes, we investigated effects on spontaneous oxidant‐induced reactive oxygen species formation human peripheral blood lymphocytes comparison the trolox, a water‐soluble analogue of α‐tocopherol. exhibited against t‐butylated hydroperoxide‐ diamide‐induced lymphocytes. turned out be three times more effective than...
It is concluded that melatonin not incorporated into LDL in sufficient concentrations to prevent lipid peroxidation effectively. When present the incubation medium during oxidation, a partitioning equilibrium between aqueous and phase established. Only under these conditions can act as chain breaking antioxidant. The required, however, are far beyond those found human plasma. Therefore, data this study do support direct physiological relevance of an antioxidant processes.
Abstract Background Oxidative stress occurs during strenuous physical exercise, perhaps as a result of increased consumption oxygen. Materials and methods In this study, different markers oxidative were determined in eight national league American football players. Before (March) at three time‐points the competition season (May, June, July) serum total peroxide concentrations, autoantibody titres against oxidized low‐density lipoprotein (oLab), lag time reactive oxygen species‐induced...
Kinetic simulation can help obtain deeper insight into the molecular mechanisms of complex processes, such as lipid peroxidation (LPO) in low-density lipoprotein (LDL). We have previously set up a single-compartment model this process, inititating with radicals generated externally at constant rate to show interplay radical scavenging and chain propagation. Here we focus on initiating events, substituting initiation (Ri) by redox cycling Cu2+ Cu+. Our reveals that early events...