CRISPR-Cas9 has emerged as a highly effective and customizable genome editing tool, holding significant promise for the treatment of KRAS mutations in lung cancer. In this study, we introduce novel micelleplex, named C14-PEI, designed to co-deliver Cas9 mRNA sgRNA efficiently excise mutated allele cancer cells. C14-PEI is synthesised from 1,2-epoxytetradecane branched PEI 600 Da via ring-opening reaction. The resulting critical micelle concentration (CMC) approximately 20.86 ± 0.15 mg L-1,...

In this paper, we present significant progress performed on an experiment dedicated to the determination of Boltzmann constant, k, by accurately measuring Doppler absorption profile a line in gas ammonia at thermal equilibrium. This optical method based first principles statistical mechanics is alternative acoustical which has led unique k published CODATA with relative accuracy 1.7 ppm. We report measurement constant laser spectroscopy uncertainty below 10 ppm, more specifically 6.4 results...

Tall cell carcinoma with reverse polarity (TCCRP) is a rare and newly recognized subtype of invasive breast carcinoma, first described in 2003. It shares histological similarities tall papillary thyroid characterized by structures cellular polarity. Due to its rarity, TCCRP has been poorly understood, fewer than 100 cases have reported globally. This report presents the case South Tunisia, contributing growing body knowledge regarding this tumor. A 34-year-old woman no significant personal...

We sought to investigate the impact of recent violence exposure on relationship between extrafamilial adverse childhood experiences (ACEs) and self-inflicted in male Tunisian prisoners. performed a cross-sectional study among adult prisoners during April 2023. used validated Arabic versions World Health Organization ACE questionnaire Hospital Anxiety Depression Scale. assessed incarceration. Self-directed was subdivided into suicidal behaviors self-harm. The participation anonymous...

We report on the growth, fabrication, experimental study and application in an absorption gas setup of distributed feed-back antimonide diode lasers with buried grating. First, half laser structures were grown by molecular beam epitaxy GaSb substrates stopped at top waveguide. A second order Bragg grating was then defined interferometric lithography structure dry etched Reactive Ion Etching. The was, afterwards, thanks to epitaxial regrowth cladding layer. Finally, wafer processed using...

In this paper we present an accurate analysis of the shape isolated rovibrational ammonia line from strong nu2 band around 10 $\mu$m, recorded by laser absorption spectroscopy. Experimental spectra obtained under controlled temperature and pressure, are confronted to various models that take into account Dicke narrowing or speed-dependent effects. Our results show clear evidence for broadening shifting, which had never been demonstrated so far in NH3. Accurate lineshape parameters saQ(6,3)...

An antimonide distributed feedback quantum wells diode laser operating at 3.32 μm near room temperature in the continuous wave regime has been used to perform ethylene detection based on quartz enhanced photoacoustic spectroscopy. absorption line centered 3007.52 cm−1 was investigated and a normalized noise equivalent coefficient (1σ) of 3.09 10−7 W Hz−1/2 obtained. The linearity stability have evaluated. Biological samples’ respiration measured validate feasibility setup an agronomic...

We report on our on-going effort to measure the Boltzmann constant, kB, using Doppler broadening technique. The main systematic effects affecting measurement are discussed. A revised error budget is presented in which global uncertainty reduced 2.3 ppm. This corresponds a reduction of more than one order magnitude compared with previous constant measurement. Means reach determination kB at part per million accuracy level outlined.

Hepatic injuries have been reported in patients with Coronavirus disease 2019 infection, particularly those moderate to severe illness. To date, pathological changes caused by acute respiratory syndrome coronavirus 2 (SARS-CoV-2) liver tissue are unclear. Moreover, the mechanisms involved injury infection not yet established. In this paper, we summarize spectrum of pathologic findings infected SARS-CoV-2 and discuss clinicopathological correlation damage infection.

BACKGROUND: Breast cancer (BC) patients’ diagnosis and management was affected by a global reorganization after the Coronavirus disease 2019 (COVID-19). Our study aimed to assess impact of pandemic on pathological stage newly diagnosed patients with BC compared pre-pandemic identify predictive factors tumor advanced stage. METHODS: Pathological records all consecutive operated between March 2020 December 2021 were reviewed retrospectively. Clinical prognostic collected periods. Then,...

We report first results on the coupling by optical-feedback of a vertical-external-cavity surface-emitting laser to high finesse optical cavity obtain sensitivity absorption measurements around 2.3 μm. This was possible thanks mechanically stiff, short-cavity (0.3 mm) design allowing broad tuning capabilities together with low frequency noise. With respect other semiconductor sources, we achieved comparable (base line noise 9 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML"...

We report on single-frequency operation and wide mode-hop-free tuning range of a diode laser-pumped vertical-external-cavity surface-emitting semiconductor laser producing up to 3 mW around 2.3 mum. An optically stable external cavity only 1.4 mm is formed using concave dielectric mirror 2-mm radius. The maximum continuous about 500 GHz wide, obtained by synchronized ramp length the gain medium temperature. used this tunable source record direct absorption spectrum methane, which found match...

This paper deals with thermal attenuation of a transmitted THz signal (165 GHz) across POM polymer sample subjected to gradient in the thickness direction. The describes experimental setup, including sensor, system impose loading and an infrared camera used measure temperature variations at surface. dependence through was studied along spatial profile. A simple polynomial model, validated finite element analysis imaging measurement, estimate correspondence between 2D 3D distribution allowed...

We report on the modeling, growth, processing, characterization and integration in a gas detection setup of side wall corrugated distributed feed-back antimonide diode lasers emitting at 2.28 2.67 μm. The laser structures were grown by molecular beam epitaxy GaSb substrate. Ridge fabricated from wafers according to following process: second order Bragg grating was defined sides ridges interferometric lithography, optical lithography etched Cl-based inductively coupled plasma reactor. devices...

Background: Congenital epulis is a benign gingival tumor whose differential diagnosis includes other oral-facial masses such as teratoma, hemangioma, lymphatic malformation and dermoid cysts. This can cause obstruction of the airway or feeding problems in newborn. Surgical excision treatment choice. Case Report: We present case congenital epulis, diagnosed prenatally with ultrasonography. Conclusion: Although difficult, defined prenatal image possible by means accurate high-resolution It...

Kaposiform hemangioendothelioma is a rare, borderline tumor that typically occurs during early childhood. Kasabach-Merritt phenomenon complication characterized by consumptive coagulopathy resulting from the localized intravascular coagulation. Hypercalcemia generation rare association was related in our case to parathyroid hormone-related protein produced this angioma.

Inflammatory myofibroblastic tumors (IMT) are rare borderline with a variable histological appearance that may mimic multiple mesenchymal tumors. We present case of challenging abdominal mass discovered in premature newborn. The histopathology showed bland proliferation associated an inflammatory infiltrate was positive for smooth muscle actin and desmin but negative anaplastic lymphoma kinase (ALK) protein. diagnosis ALK-negative IMT established. tumor partially resected. After six months...