A5103219842- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
- CAR-T cell therapy research
- Microtubule and mitosis dynamics
- Ovarian cancer diagnosis and treatment
- Optical Systems and Laser Technology
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- Single-cell and spatial transcriptomics
- Caveolin-1 and cellular processes
- Ubiquitin and proteasome pathways
- Image Processing Techniques and Applications
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
Science for Life Laboratory
2021-2025
Karolinska Institutet
2021-2025
Svenska Örtmedicinska Institute
2025
Farnesylation is a lipid post-translational modification of proteins crucial for protein membrane anchoring and cellular signaling. Farnesyltransferase inhibitors (FTIs), such as tipifarnib, are being tested in cancer therapy. However, the full impact FTIs on farnesylation substrates remains poorly understood, thus limiting their use precision medicine. In this study, we performed global proteomics analysis to investigate effects tipifarnib lung cell lines. Using metabolic labeling mass...
Abstract Single-cell image analysis is crucial for studying drug effects on cellular morphology and phenotypic changes. Most studies focus single cell types, overlooking the complexity of interactions. Here, we establish an pipeline to extract features cancer cells cultured with fibroblasts. Using high-content imaging, analyze oncology library across five fibroblast line co-culture combinations, generating 61,440 images ∼170 million single-cell objects. Traditional phenotyping CellProfiler...
Abstract Introduction: Clinical trials of mesothelin (MSLN) chimeric antigen receptor (CAR) T-cell therapy have shown limited efficacy in solid tumors like ovarian cancer (OVCA), emphasizing the need to enhance CAR T cell performance. This study evaluated whether combining MSLN-CAR cells with drugs, either sequentially or concurrently, could achieve synergistic anti-tumor effects. Methods :MSLN-directed were generated using two constructs CD28 (M28z) 4-1BB (MBBz) co-stimulatory domains. A...
Abstract Ovarian cancer (OC) is a leading cause of gynecological cancers deaths in women. Despite high histological and genetic heterogeneity OC, the standard treatment remains largely unchanged, consisting cytoreductive surgery followed by platinum-taxol chemotherapy. The introduction PARP inhibitor OC shows some advancements, but relapse rates acquired resistance to secondary continue contribute poor 5-year survival rate, highlighting need for improved personalized strategies. Hence this...
Most patients with advanced ovarian cancer (OC) relapse and progress despite systemic therapy, pointing to the need for improved tailored therapy options. Functional precision medicine can help identify effective therapies individual in a clinically relevant timeframe. Here, we present scalable functional platform: DET3Ct (Drug Efficacy Testing 3D Cultures), where response of patient cells drugs drug combinations are quantified live-cell imaging. We demonstrate delivery sensitivity profiles...
Abstract Most patients with ovarian cancer (OC) are diagnosed at a late stage when there very few therapeutic options and poor prognosis. This is due to the lack of clearly defined underlying mechanisms or an oncogenic addiction that can be targeted pharmacologically, unlike other types cancer. Here, we identified protein tyrosine kinase 7 (PTK7) as potential new target in OC following multiomics approach using genetic pharmacological interventions. We performed proteomics analyses upon PTK7...
Replicative repair of interstrand crosslinks (ICL) generated by platinum chemotherapeutics is orchestrated the Fanconi anemia (FA) pathway to ensure resolution stalled replication forks and maintenance genomic integrity. Here, we identify novel regulation FA cancer-associated glycolytic enzyme PFKFB3 that has functional consequences for replication-associated ICL cancer cell survival. Inhibition displays a cancer-specific synergy with compounds in blocking viability restores sensitivity...
<h3>Background</h3> Chimeric antigen receptor (CAR) T cell therapy has shown efficacy in several hematologic malignancies, either as monotherapy or a bridge to allogeneic hematopoietic transplantation.<sup>1–4</sup> Despite this success, some patients do not respond treatment and others relapse within 1 year of treatment.<sup>5</sup> Additionally, efforts are underway extend CAR solid tumors, with the glycoprotein mesothelin (MSLN) emerging selective target ovarian cancer...
Abstract Most patients with advanced ovarian cancer (OC) relapse and progress despite systemic therapy, pointing to the need for improved tailored therapy options. Functional precision medicine can help identify effective therapies individual in a clinically relevant timeframe. Here, we present scalable functional platform: DETECt (Drug Efficacy Testing Ex vivo 3D Cultures), where responses of primary patient cells drugs drug combinations are tested live-cell imaging. We demonstrate delivery...
Abstract Many ovarian cancer (OC) patients receiving chemotherapy relapse with acquired resistance, hence novel treatment allocation strategies are urgently needed. 3D cell culture models and organoid technologies have emerged as exciting tools to study cancer. Despite the advances, there is a need for protocols that can quickly generate patient-relevant high-throughput drug screening diagnostic sensitivity testing. Ideally, such methods should be cost-effective, provide multi-parametric...