A5046733214- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Marine and fisheries research
- Reproductive biology and impacts on aquatic species
- Crustacean biology and ecology
- Seismic Performance and Analysis
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Coral and Marine Ecosystems Studies
- Vibration Control and Rheological Fluids
- Structural Health Monitoring Techniques
- RNA Interference and Gene Delivery
- Biochemical Analysis and Sensing Techniques
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Meat and Animal Product Quality
- Protein Hydrolysis and Bioactive Peptides
Kyoto University
2015-2024
Kindai University
2021-2022
Nagahama Institute of Bio-Science and Technology
2022
National Institute for Basic Biology
2017
Kyoto Architecture University
2015
Tokyo University of Marine Science and Technology
2011-2014
Oil, hysteretic and inertial mass dampers are representatives of passive used for smart enhancement seismic performance building structures. Since oil have a nonlinear relief mechanism possess restoring-force characteristics, several difficulties arise in the evaluation buildings including such dampers. The purpose this paper is to propose practical method simultaneous optimal use optimum design problem formulated so as minimize maximum interstory drift under earthquakes terms set damper...
The CRISPR/Cas system is a powerful genome editing tool that enables targeted modifications in various organisms. In medaka (Oryzias latipes), mutagenesis with small insertions and deletions using this have become robust technique are now widely used. However, to date there been only number of reports on gene integration system. We thus sought the present study identify factors enhance efficiency events medaka.We show longer homology arms (ca. 500 bp) linearization circular donor plasmids by...
Myostatin (MSTN) is a negative regulator of skeletal muscle growth and popular target for enhancing the productivity farmed fish. We previously developed an mstn-knockout breed aquaculture fish red sea bream (Pagrus major) using genome editing technology. However, little known about effects mstn disruption on fillet quality other species. In this study, we used fillets mstn-deficient to evaluate their compositional textural changes during refrigeration. Compared wild type, mutant exhibited...
To determine the early life history of land hermit crab Coenobita brevimanus Dana, 1852, larvae were cultured individually in laboratory. The zoeal and megalopal stages are described illustrated. developed through four planktonic to stage. major differences characters between C. other species found armature pleomeres, whereas character pleomeres zoeae is same as that coconut Birgus latro, a different genus family. Morphological similarity was also segmentations antennules antennae megalopae...
Abstract The CRISPR/Cas system offers new opportunities for targeted gene modifications in a wide range of organisms. In medaka ( Oryzias latipes ), vertebrate model organism, wild‐type Cas9‐based approach is commonly used to establish desired strains, however, its use lethal genes still challenging due excess disruptions triggered by DNA double strand breaks (DSBs). To overcome this problem, we aimed develop knock‐in using Cas9 nickase (Cas9n) that can reduce DSBs. We revealed Cas9n allowed...
Abstract In this study, we developed a novel bioreactor system to deliver and accumulate foreign proteins in eggs using medaka fish Oryzias latipes with the aid of partial sequence vitellogenin (Vtg). teleost fish, Vtg, hepatically generated precursor egg yolk proteins, is secreted into bloodstream then taken up eggs. We predicted silico probable region (Vtg signal) Vtg that mediates transportation from liver Then, established two transgenic lines expressing fused including signal each...
Summary Homology‐directed repair (HDR)‐mediated genome editing has become a powerful method for altering chromosomal sequences in seamless and accurate manner. However, the low efficiency of HDR most cells hinders establishment desired strains harboring accurately modified genomes. To enhance HDR‐mediated knock‐in events, we explored two approaches, namely low‐temperature incubation chemical compound administration using medaka embryos after microinjection. We validated performance each by...
This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus.This method makes it possible to determine genotype each individual simply by observing fluorescence later than F1 generation.
Abstract Inosine monophosphate (IMP) is an important indicator of meat freshness and contributes to its umami taste. An attractive strategy for enhancing suppress the IMP-degrading activity increase IMP content in skeletal muscle through genome editing technology using CRISPR-Cas9 system. However, molecular mechanisms underlying degradation remain unclear. We cloned two ecto-5′-nucleotidase genes, designated as - a ( nt5ea ) b nt5eb ), from medaka Oryzias latipes vertebrate model organism....
We described a simple method for microinjecting DNA/RNA/Protein solutions into medaka eggs under an upright microscope. Medaka is excellent vertebrate model reverse genetics, because of its daily spawning, short generation time, and egg transparency. These features enable us to efficiently perform functional genomic analyses transgenic or genome edited fish. This protocol contains the initial steps necessary create various types genetically modified
The article "Development of an efficient bioreactor system for delivering foreign proteins secreted from liver into eggs with a vitellogenin signal in medaka Oryzias latipes", written by Yu Murakami, Tomohisa Horibe and Masato Kinoshita was originally published electronically on the publisher's internet portal 21 May 2019 without open access. After publication volume [85], issue [4], page [677-685] author(s)' decision to opt Open Choice copyright changed 31st March 2020 Author(s) is...