A5007390701- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- Genetic diversity and population structure
- Fish Biology and Ecology Studies
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Aquaculture disease management and microbiota
- Animal Behavior and Reproduction
- Zebrafish Biomedical Research Applications
- Fish Ecology and Management Studies
- Retinal Development and Disorders
- Fish biology, ecology, and behavior
- Identification and Quantification in Food
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Hypothalamic control of reproductive hormones
- Primate Behavior and Ecology
- Physiological and biochemical adaptations
- Amphibian and Reptile Biology
- Aquaculture Nutrition and Growth
- Evolutionary Psychology and Human Behavior
- Plant and animal studies
Okayama University
2024-2025
Marine Institute
2025
Okayama University of Science
2025
Kyoto University
2012-2024
National Institute for Basic Biology
2017-2024
National Institute of Genetics
2017-2024
Tohoku University
2020-2024
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonuclease (RGEN) has recently emerged as a simple and efficient tool for targeted genome editing. In this study, we showed successful mutagenesis using RGENs in medaka, Oryzias latipes. Somatic heritable mutations were induced with high efficiency at the genomic sequence on DJ-1 gene embryos that had been injected single guide RNA (sgRNA) transcribed by T7 promoter capped...
Transcription activator-like effector nucleases (TALENs) have become powerful tools for targeted genome editing. Here we demonstrate efficient mutagenesis in medaka (Oryzias latipes), which serves as an excellent vertebrate model genetics and genomics. We designed constructed a pair of TALENs targeting the DJ-1 gene, homolog human (PARK7). These induced number insertions deletions injected embryos with extremely high efficiency. This induction mutations occurred dose-dependent manner. All...
Abstract Teleost fishes exhibit complex sexual characteristics in response to androgens, such as fin enlargement and courtship display. However, the molecular mechanisms underlying their evolutionary acquisition remain largely unknown. To address this question, we analyse medaka ( Oryzias latipes ) mutants deficient teleost-specific androgen receptor ohnologs ara arb ). We discovered that neither ar ohnolog was required for spermatogenesis, whilst they appear be functionally redundant...
To increase individual male fitness, males of various species remain near a (potential) mating partner and repel their rivals (mate-guarding). Mate-guarding is assumed to be mediated by two different types motivation: sexual motivation toward the opposite sex competitive same sex. The genetic/molecular mechanisms underlying how mate presence affects in triadic relationship has remained largely unknown. Here we showed that medaka fish prominently exhibit mate-guarding behavior. female...
Genome editing using engineered nucleases such as transcription activator‐like effector ( TALEN s) has become a powerful technology for reverse genetics. In this study, we have described efficient detection methods ‐induced mutations at endogenous loci and presented guidelines of design targeted mutagenesis in medaka, Oryzias latipes . We performed heteroduplex mobility assay HMA ) an automated microchip electrophoresis system, which is simple high‐throughput method evaluation vivo activity...
Homozygous mutations in the glucocerebrosidase (GBA) gene result Gaucher disease (GD), most common lysosomal storage disease. Recent genetic studies have revealed that GBA confer a strong risk for sporadic Parkinson's (PD). To investigate how cause PD, we generated nonsense mutant (GBA-/-) medaka are completely deficient (GCase) activity. In contrast to perinatal death humans and mice lacking GCase activity, GBA-/- survived months, enabling analysis of pathological progression. displayed...
Oxytocin is a central neuromodulator required for facilitating mate preferences familiar individuals in monogamous rodent (prairie vole), irrespective of sex. While the role oxytocin choice only understood few species, its function nonmonogamous comprising vast majority vertebrate remains unclear. To address this issue, we evaluated involvement an homolog (isotocin, referred herein as oxt) medaka fish ( Oryzias latipes ). Female prefer to choose mates, whereas male court indiscriminately,...
Abstract Sexual selection drives rapid phenotypic diversification of mating traits. However, we know little about the causative genes underlying divergence in sexually selected Here, investigate genetic basis male trait medaka fishes (genus Oryzias ) from Sulawesi, Indonesia. Using linkage mapping, transcriptome analysis, and genome editing, identify csf1 as a gene for red pectoral fins that are unique to woworae . A cis -regulatory mutation enables androgen-induced expression fins....
Male and female animals display innate sex-specific mating behaviors. In teleost fish, altering the adult sex steroid milieu can effectively reverse sex-typical behaviors, suggesting remarkable sexual lability of their brains as adults. medaka, neuropeptide B (NPB) is expressed female-specifically in brain nuclei implicated behavior. Here, we demonstrate that NPB a direct mediator estrogen action on behavior, acting female-specific but reversible manner. Analysis regulatory mechanisms...
Abstract The genus Oryzias consists of 35 medaka-fish species each exhibiting various ecological, morphological and physiological peculiarities adaptations. Beyond being a comprehensive phylogenetic group for studying intra-genus evolution several traits like sex determination, behavior, morphology or adaptation through comparative genomic approaches, all medaka share many advantages experimental model organisms including small size short generation time, transparent embryos genome editing...
Seasonal changes in metabolism are crucial for animals to adapt annual environmental fluctuations. However, the molecular mechanisms underlying these adaptations remain poorly understood. Here, we identified a novel gene, photoperiod decoder 1 ( phod1 ), which exhibits unique bimodal expression pattern under long-day conditions Japanese medaka fish Oryzias latipes ). While is conserved across many vertebrates, except eutherians, its function remained unknown. Single-cell RNA sequencing...
Abstract Skeletal forms in vertebrates have been regarded as good models of morphological diversification. Fish fin are greatly diversified, and their bone structure is classified into soft rays spiny rays. In fish evolution, spiny-ray morphologies known to be sometimes extremely modified; however, it remains unknown how the developmental mechanisms spiny-rays contributed By using rainbowfish Melanotaenia praecox for examination extracellular matrix (ECM) cell dynamics development, we...
GCaMP-based calcium imaging is a powerful tool for investigating neural function in specific neurons. We generated transgenic (Tg) medaka strains expressing jGCaMP7s across extensive brain regions under the control of gap43 promoter. Using these Tg larvae, successfully detected tricaine-induced suppression spontaneous activity and topographical visual responses optic tectum elicited by moving paramecia or optical fiber stimulation. These results indicate that our provide versatile platform...
Male-male competition and female mating preference are major mechanisms of sexual selection, which influences individual fitness. How male-male affects preference, however, remains poorly understood. Under laboratory conditions, medaka (Oryzias latipes) males compete to position themselves between a rival male the (mate-guarding) in triadic relationships (male, male, female). In addition, females prefer mate with visually familiar males. present study, examine whether mate-guarding via...
The CRISPR/Cas system is a powerful genome editing tool that enables targeted modifications in various organisms. In medaka (Oryzias latipes), mutagenesis with small insertions and deletions using this have become robust technique are now widely used. However, to date there been only number of reports on gene integration system. We thus sought the present study identify factors enhance efficiency events medaka.We show longer homology arms (ca. 500 bp) linearization circular donor plasmids by...
Despite drastic cellular changes during cleavage, a mitotic spindle assembles in each blastomere to accurately segregate duplicated chromosomes. Mechanisms of assembly have been extensively studied using small somatic cells. However, mechanisms large vertebrate embryos remain little understood. Here, we establish functional assay systems medaka (Oryzias latipes) by combining CRISPR knock-in with auxin-inducible degron technology. Live imaging reveals several unexpected features microtubule...
Sexual dimorphism allows species to meet their fitness optima based on the physiological availability of each sex. Although intralocus sexual conflict appears be a genetic constraint for evolution sex-specific traits, sex-linked genes and regulation sex steroid hormones contribute resolving this by allowing developments. Androgens receptor, androgen receptor (Ar), regulate male-biased phenotypes. In teleost fish, ar ohnologs have emerged as result teleost-specific whole genome duplication...
Zinc‐finger nucleases ( ZFN s) are artificial enzymes that create site‐specific double‐strand breaks and thereby induce targeted genome editing. Here, we demonstrated successful gene disruption in somatic germ cells of medaka O ryzias latipes ) using to target exogenous EGFP genes. Embryos were injected with an RNA sequence pair coding for s showed mosaic loss green fluorescent protein fluorescence skeletal muscle. A number mutations included both deletions insertions identified within the...
An increasing volume of empirical studies demonstrated that hybridization between distant lineages may have promoted speciation in various taxa. However, the timing, extent and direction introgressive remain unknown many cases. Here, we report a possible case which repeated divergence Oryzias ricefishes (Adrianichthyidae) on Sulawesi, an island Wallacea. Four species are endemic to Malili Lake system central is composed five tectonic lakes; these, one lake inhabited by two species....
AbstractSex chromosomes rapidly turn over in several taxonomic groups. Sex chromosome turnover is generally thought to start with the appearance of a new sex-determining gene on an autosome while old still exists, followed by fixation one. However, we do not know how prevalent transient state is, where multiple loci coexist within natural populations. Here, removed Y master male-determining DMY from medaka fish using high temperature-induced sex-reversed males. After four generations,...