Our previous studies have suggested that synthetic octacalcium phosphate (OCP) could be resorbed and replaced by newly formed bone if implanted in rat skull defects. We hypothesized the OCP is more resorbable than other commonly used graft substitutes of calcium compounds, such as hydroxyapatite (HA) beta-tricalcium (beta-TCP). To test hypothesis, present study was designed to compare histomorphometrically resorption OCP, HA, beta-TCP, which were kept experimental cranial defect rats for a...

Matrix metalloproteinases (MMPs) 8 and 13 comprise the collagenase subfamily in rats mice, only MMP13 has been implicated degradation of collagenous matrices during development bone cartilage. On hypothesis that MMP8 is also involved cartilage development, present study was designed to investigate gene expression rat embryonic mandibles hind limbs. Expression examined with situ hybridization RT-PCR compared MMP13. Osteoblastic chondrocytic cells expressing matrix molecules were identified...

Synthetic octacalcium phosphate (OCP) enhances bone formation if implanted into the subperiosteal region of murine bone. Such OCP may be resorbed and replaced by with time. We hypothesized that could used as an effective substitute. To test this hypothesis, we designed present study to investigate repair in a rat skull defect is enhanced implantation OCP. Rats were divided two groups: OCPtreated animals untreated controls. Six rats from each group fixed at 4, 12, 24 weeks after implantation....

Periodontal ligament tissue is remodeled on both the tension and compression sides of moving teeth during orthodontic tooth movement. The present study was designed to clarify hypothesis that expression MMP-8 MMP-13 mRNA promoted remodeling periodontal in We used situ hybridization method semi-quantitative reverse-transcription/polymerase chain-reaction analysis elucidate gene mRNA. Expression transiently increased active movement vivo. induced by tension, while indirectly through soluble...

Bone formation in vivo occurs via two major processes, one of which depends on pre-existing cartilage, and the other does not. morphogenetic proteins (BMPs) have been suggested to induce cartilage from non-skeletogenic mesenchymal cell population, results osteogenesis through endochondral sequence. In present study we examined if BMPs could cause direct bone independent using bovine fibrous collagen membrane (FCM) as a carrier for BMPs. Bovine metatarsal was extracted 4 M guanidine HC1 were...

Abstract Skeletal forms in vertebrates have been regarded as good models of morphological diversification. Fish fin are greatly diversified, and their bone structure is classified into soft rays spiny rays. In fish evolution, spiny-ray morphologies known to be sometimes extremely modified; however, it remains unknown how the developmental mechanisms spiny-rays contributed By using rainbowfish Melanotaenia praecox for examination extracellular matrix (ECM) cell dynamics development, we...

Extracellular matrix (ECM) remodeling is achieved by both production and degradation of ECM molecules during bone development. ADAMTS (a disintegrin metalloprotease with thrombospondin type 1 motifs) constitutes a family extracellular proteases which are implicated in cleaving the protein versican. The present study was designed to investigate expression versican ADAMTS1, 4, 5 mRNA development rat mandibles hind limbs RT-PCR situ hybridization. Versican localized immunohistochemistry....

The ultrastructural characteristics of multinucleated giant cells (MNGCs) on octacalcium phosphate (OCP) and hydroxyapatite (HA) were investigated in comparison with those osteoclasts, when the synthetic OCP HA implanted rat bone marrow. morphological difference MNGCs shown between implants 2 weeks after implantation. MNGC (i-OCP) developed ruffled border-like structure clear zone-like structure. i-OCP was frayed where it contact (i-HA) structure, whereas no border seen. surface i-HA...

A bone defect that is not repaired with completely designated a non-union or critical-size defect. The biological mechanism regulates the process of repair remains unknown. present study was designed to investigate in compared smaller non-critical-size Our original standardized rat calvarial model used for experiment. rate formation examined X-ray morphometry and production osteoblasts osteocytes assessed by molecular histology situ hybridization type I collagen osteocalcin. Formation ceased...

The present study was designed to investigate mRNA expression of matrix metalloproteinase-8 (MMP-8) and MMP-13 in forming periodontium during tooth eruption the rat. RT-PCR for decalcified paraffin sections indicated MMP-8 periodontal tissues. In situ hydridization demonstrated osteoblasts, osteocytes, ligament cells, cementoblasts, cementocytes along with collagen types I III. contrast, transcripts were confined a small population osteoblasts osteocytes alveolar bone. results suggested that...

Abstract Our previous studies suggested that a part of bone extracellular matrix (ECM) molecules are degraded and remodeled during embryonic formation. In contrast, little is known about ECM remodeling in postnatal appositional The present study was designed to investigate expression metalloproteinases (MMPs) tissue inhibitors (TIMPs) experimentally initiated formation rats. Expressions molecules, MMPs, TIMPs were examined using situ hybridization. Osteoblasts osteocytes expressed MMP2 ‐8,...

Abstract A bioactive and resorbable scaffold is necessary to exhibit the osteoinductive potency of recombinant human bone morphogenetic protein‐2 (rhBMP‐2). In a previous study, we found that synthetic octacalcium phosphate (OCP) enhances regeneration replaced by newly formed after it resorbed. We hypothesized OCP may be useful as an effective for rhBMP‐2 enhance regeneration. To test this hypothesis, present study was designed investigate whether OCP/BMP composite implant could more...

Abstract The study examined the expression of matrix metalloproteinases (MMPs), type I collagen and osteocalcin during bone healing in a rat calvarial experimental defect model. Twelve‐week‐old male Wistar rats were used. A full‐thickness standardized trephine was made parietal bone, with under anesthesia. RNA extracted from tissue that filled original on days 1 3 weeks 1, 2, 3, 5, 8, 10, 12, 18, 24 processed for quantitative analysis collagen, (MMPs) 13 by using real‐time polymerase chain...

Piezosurgery is an innovative technique widely used for osteotomies in the field of oral and maxillofacial surgery. The surgical has been clinically supposed to cut mineralized bone selectively with reducing risk damage adjacent soft tissues. However, none previous literature reported any evidence scientific experiments examine performance piezoelectric device, i.e. time required cutting effect on tissues under standardized conditions. This study was designed test hypothesis that device...

It is not known whether long bones and calvaria have distinct biological characteristics. Octacalcium phosphate (OCP), which a precursor phase of the hydroxyapatite, has been reported to stimulate bone formation if implanted in subperiosteal region mouse calvaria. The present study was designed investigate how calvarium respond OCP implantation compare their characteristics.The synthetic into rat tibiae parietal being mixed with bovine type I collagen treated by pepsin (Atelocollagen)....

The mechanical property of immobilized joints is not well understood. present study was designed to investigate the tissue elasticity anterior and posterior synovial membrane (SM) in a rat knee model using scanning acoustic microscopy (SAM). Moreover, structural characteristics SM after immobilization were examined by transmission electron (TEM).Thirty rats had their with plate metal screws. fixed at 1, 2, 4, 8 16 weeks surgery sectioned sagittally for SAM. Selected specimens processed TEM....