A5026762657- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Physiological and biochemical adaptations
- Retinal Development and Disorders
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Erythrocyte Function and Pathophysiology
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Neuroscience and Neural Engineering
- Microtubule and mitosis dynamics
- Neurobiology and Insect Physiology Research
- Regulation of Appetite and Obesity
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Fish biology, ecology, and behavior
- Biochemical Analysis and Sensing Techniques
- Genetic and Kidney Cyst Diseases
- Gastrointestinal motility and disorders
- Neuroendocrine regulation and behavior
- RNA Research and Splicing
- Diet, Metabolism, and Disease
- Receptor Mechanisms and Signaling
Saitama University
2017-2025
Nanyang Technological University
2014-2016
Institute of Molecular and Cell Biology
2014-2016
Duke-NUS Medical School
2010-2014
Agency for Science, Technology and Research
2012-2014
Marine Biological Laboratory
2012-2014
The University of Tokyo
2007-2010
Tokyo University of Science
2010
Tohoku University
1995
Kyushu University
1972-1975
Recent studies have revealed that a cilium‐generated liquid flow in the node has crucial role establishment of left‐right (LR) axis mouse. In fish, Kupffer's vesicle (KV), teleost‐specific spherical organ attached to tail region, is known an equivalent mouse during LR formation. However, at present, there been no report asymmetric gene expressed KV under control fluid flow. Here we earliest teleost KV, medaka charon , and its regulation. Charon member Cerberus/DAN family proteins, first...
Here we characterize several new lines of transgenic mice useful for optogenetic analysis brain circuit function. These express probes, such as enhanced halorhodopsin or different versions channelrhodopsins, behind various neuron-specific promoters. permit photoinhibition photostimulation both in vitro and vivo. Our results also reveal the important influence fluorescent tags on probe expression function mice.
We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us focally photostimulate these neurons, while measuring resulting responses postsynaptic Purkinje cells. This approach revealed that converge upon cells over a broad area and at least seven form functional synapses with single cell. The number...
GCaMP-based calcium imaging is a powerful tool for investigating neural function in specific neurons. We generated transgenic (Tg) medaka strains expressing jGCaMP7s across extensive brain regions under the control of gap43 promoter. Using these Tg larvae, successfully detected tricaine-induced suppression spontaneous activity and topographical visual responses optic tectum elicited by moving paramecia or optical fiber stimulation. These results indicate that our provide versatile platform...
During the development of vertebrate nervous system, mitosis neural progenitor cells takes place near lumen, apical side tube, through a characteristic movement nuclei known as interkinetic nuclear migration (INM). Furthermore, during proliferative period, exhibit planar cell divisions to produce equivalent daughter cells. Here, we examine potential role extracellular signals in INM and using medaka mutant tacobo (tab). This tab shows pleiotropic phenotypes, including neurogenesis,...
Abstract Optical measurement of membrane potentials enables fast, direct and simultaneous detection from a population neurons, providing desirable approach for functional analysis neuronal circuits. Here, we applied recently developed genetically encoded voltage indicators, ASAP1 (Accelerated Sensor Action Potentials 1) QuasAr2 (Quality superior to Arch 2), zebrafish, an ideal model system studying neurogenesis. To achieve this, established transgenic lines which express the sensors, showed...
Germinating seeds of many species contain two types β‐cyanoalanine synthase (CAS, EC 4.4.1.9) that convert HCN to β‐cyanoalanine. One is cytoplasmic CAS (cyt‐CAS), which precipitated by 50 60% (NH 4 ) 2 SO and has a pH optimum 10.5. Cytoplasmic present at high levels in dry seed its activity does not increase during imbibition. The cyt‐CAS affected exogenously applied ethylene (C H ), except rice ( Oryza sativa cv. Sasanishiki). second type found mitochondrial (mit‐CAS), 60 70% 9.5....
Abstract We have isolated and characterized a ventralized mutant in medaka (the Japanese killifish; Oryzias latipes ), which turned out to mutation the chordin gene. The exhibits ventralization of body axis, malformation axial bones, over‐bifurcation yolk sac blood vessels, laterality defects internal organs. variability phenotypes, depending on culture temperature, from embryos with slightly phenotype those without any head trunk structures. Taking advantages these variable severe we...
Ghrelin is a unique fatty acid-modified peptide hormone produced in the stomach and has important roles energy homeostasis gastrointestinal motility. However, medium-chain acid source for ghrelin acyl-modification not known. We found that fat-free diet removal of intestinal microbiota did decrease acyl-ghrelin production or plasma levels mice. RT-PCR analysis showed genes involving synthesis, metabolism, transport were expressed pancreas-derived ghrelinoma (PG-1) cells. Treatment with an...
Voltage-sensitive dye (VSD) imaging enables fast, direct, and simultaneous detection of membrane potentials from a population neurons forming neuronal circuits. This the hyperpolarization together with depolarization, whose balance plays pivotal role in function many brain regions. Among these is cerebellum, which contains significant number inhibitory neurons. However, mechanism underlying functional development remains unclear. In this study, we used model system ideal to study...
G protein-coupled receptor 139 (GPR139), a highly conserved orphan receptor, is predominantly expressed in the habenula of vertebrate species. Habenula an ancient epithalamic structure, which critical to comprehending adaptive behaviors vertebrates. We have previously demonstrated role GPR139 agonists fear-associated decision-making processes zebrafish. However, how signaling modulates such behavioral responses remains unsolved. Fish centrally administered with synthetic antagonist for human...
Dry seeds can transform volatile metabolites via enzymes pre-existing in them. The interconversion between acetaldehyde and ethanol occurred when they were applied gaseously to of rice, lettuce, pea, cocklebur during storage at different relative humidities (RH) either 23°C or −3.5°C. Interconversions the compounds decreased with decreasing RH from 75% 12% regardless seed species temperature, but it was still detectable even However, conversion did not occur killed by heating prior storage....
Introduction During the morphogenesis of neural tube, progenitors continuously divide, and post mitotic neurons then migrate to form complex networks. It is widely conserved among vertebrates that this process takes place near lumen, apical side tube (Gotz Huttner, 2005). When viewed at cellular level, apically localized mitosis region output for cell-cycle-dependent oscillation nuclei within neuroepithelium. Mitotic (M phase) are located in close proximity surfaces, whereas undergoing DNA...
In the fasted gastrointestinal (GI) tract, a characteristic cyclical rhythmic migrating motor complex (MMC) occurs in an ultradian rhythm, at 90–120 min time intervals, many species. However, underlying mechanism directing this MMC pattern is yet to be completely elucidated. Therefore, study aimed identify possible causes or factors that involve occurrence of fasting gastric contractions by using Suncus murinus small model animal featuring almost same as found humans and dogs. We observed...
Stomach contractions show two types of specific patterns in many species, that is migrating motor contraction (MMC) and postprandial (PPCs), the fasting fed states respectively. We found gastric PPCs terminated with strong humans, dogs suncus. In this study, we reveal detailed characteristics physiological implications these PPC.Human, suncus canine were recorded a motility-monitoring ingestible capsule strain-gauge force transducer. The response motilin ghrelin its receptor antagonist on...
The brain consists of neural circuits, which are assemblies various neuron types. For understanding how the works, it is essential to identify functions each type and neuronal circuits. Recent advances in our function its development have been achieved using light detect activity. Optical measurement membrane potentials through voltage imaging a desirable approach, enabling fast, direct, simultaneous detection population neurons. Its high speed directness can help synaptic action...
The cerebellum, a brain region with high degree of plasticity, is pivotal in motor control, learning, and cognition. cerebellar reserve the capacity cerebellum to respond adapt various disorders via resilience reversibility. Although structural functional recovery has been reported mammals attracted attention regarding treatments for dysfunction, such as spinocerebellar degeneration, regulatory mechanisms are largely unidentified, particularly at circuit level. Herein, we established an...
Establishment of robust gene expression boundary is crucial for creating elaborate morphology during development. However, mechanisms underlying formation have been extensively studied only in a few model systems. We examined the establishment zic1/zic4-expression demarcating dorsoventral entire trunk medaka fish (Oryzias latipes) and identified subgroup dermomyotomal cells called horizontal (HBCs) as players formation. Embryological genetic analyses demonstrated that HBCs play roles two...