A5081699113- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Effects of Vibration on Health
- Ergonomics and Musculoskeletal Disorders
- Tactile and Sensory Interactions
- Chalcogenide Semiconductor Thin Films
- Genetics and Neurodevelopmental Disorders
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- 2D Materials and Applications
- Regulation of Appetite and Obesity
- Advanced Thermoelectric Materials and Devices
- Semiconductor materials and interfaces
- T-cell and B-cell Immunology
- Color perception and design
- Neuropeptides and Animal Physiology
- Health, Environment, Cognitive Aging
- Zebrafish Biomedical Research Applications
- Graphene research and applications
The University of Tokyo
2023
Fujita Health University
2019-2023
Nihon University
2023
Korea Institute of Science and Technology
2012-2021
Korean Association Of Science and Technology Studies
2021
Fujita Health University Hospital
2021
Korea Institute of Brain Science
2021
Kanazawa University
2004-2017
RIKEN Center for Brain Science
2006-2008
Tokyo University of Pharmacy and Life Sciences
2004
A subtle but chronic alteration in metabolic balance between amyloid-beta peptide (Abeta) anabolic and catabolic activities is thought to cause Abeta accumulation, leading a decade-long pathological cascade of Alzheimer disease. However, it still unclear whether reduction the activity brain causes neuronal dysfunction vivo. In present study, clarify possible connection neprilysin impairment synaptic cognitive functions, we cross-bred amyloid precursor protein (APP) transgenic mice (APP23)...
Abstract Aims Synaptic Ras GTPase‐activating protein 1 (SYNGAP1) regulates synaptic plasticity through AMPA receptor trafficking. SYNGAP1 mutations have been found in human patients with intellectual disability (ID) and autism spectrum disorder (ASD). Almost every individual ‐related ID develops epilepsy, approximately 50% ASD. is estimated to account for at least 1% of cases. In mouse models Syngap1 mutations, strong cognitive affective dysfunctions reported, yet some findings are...
Here we characterize several new lines of transgenic mice useful for optogenetic analysis brain circuit function. These express probes, such as enhanced halorhodopsin or different versions channelrhodopsins, behind various neuron-specific promoters. permit photoinhibition photostimulation both in vitro and vivo. Our results also reveal the important influence fluorescent tags on probe expression function mice.
Significance Impulsivity is a tendency to act with little or no forethought consideration of the consequences and major component various psychiatric disorders. However, known about brain circuits that regulate reward-related impulsivity. Our genetic manipulations reveal dopamine D2 receptors in central nucleus amygdala have crucial role impulsive behavior. By using optogenetics control neurons possess these receptors, we identified elements neural circuit contributes regulating This...
In Pavlovian fear conditioning, the lateral amygdala (LA) has been highlighted as a key brain site for association between sensory cues and aversive stimuli. However, learning-related changes are also found in upstream regions such thalamus cortex. To isolate essential neural circuit components memory association, we tested whether direct activation of presynaptic inputs LA, without participation activity, is sufficient to form mice. Photostimulation axonal projections from two main auditory...
Calpastatin is an endogenous inhibitor of calpain, intracellular calcium-activated protease. It has been suggested to be involved in molecular mechanisms long-term plasticity and excitotoxic pathways. However, functions calpastatin vivo are still largely unknown. To examine the physiological roles calpastatin, we subjected calpastatin-knockout mice a comprehensive behavioral test battery. Calpastatin-knockout showed decreased locomotor activity under stressful environments, acoustic startle...
An improved genetically encoded voltage indicator (GEVI) was achieved by altering the charge composition of region linking voltage-sensing domain GEVI to a pH-sensitive fluorescent protein. Negatively charged linker segments reduced voltage-dependent optical signal while positively linkers increased size. Arginine scanning mutagenesis size GEVI, Bongwoori, yielding signals as high 20% ΔF/F during firing action potentials. The speed this new sensor also capable optically resolving potentials...
To understand the circuitry of brain, it is essential to clarify functional connectivity among distinct neuronal populations. For this purpose, activity imaging using genetically-encoded calcium sensors such as GCaMP has been a powerful approach due its cell-type specificity. However, (Ca2+) an indirect measure activity. A more direct would be use genetically encoded voltage indicators (GEVIs) observe subthreshold, synaptic activities. The GEVI, ArcLight, which exhibits large fluorescence...
In order to attain high-performance p-type field-effect transistors (FETs) based on two-dimensional materials, it becomes imperative mitigate Fermi level pinning. Employing Bi with a low work function of ∼4.2 eV, characterized by its melting point, significant vapor pressure, and smaller density states, has been instrumental in establishing pinning-free n-type contacts. this context, we present new approach for This involves the modulation effective through bilayer electrode structure...
<i>Objectives:</i> The aim of this study was to evaluate whether nedaplatin-based concurrent chemoradiotherapy (CCRT) using high-dose-rate intracavitary brachytherapy (HDR-ICBT) is superior radiotherapy (RT) alone in patients with FIGO stage IIIb cervical cancer. <i>Methods:</i> records 41 consecutive women treated either CCRT HDR-ICBT (n = 20) or RT (nonrandomized control group, n 21) for cancer were retrospectively reviewed. activity and toxicity compared between...
The dentate gyrus (DG) plays critical roles in cognitive functions, such as learning, memory, and spatial coding, its dysfunction is implicated various neuropsychiatric disorders. However, it remains largely unknown how information represented this region. Here, we recorded neuronal activity the DG using Ca 2+ imaging freely moving mice analyzed machine learning. patterns of populations neurons enabled us to successfully decode position, speed, motion direction an open field, well current...
This study aimed to explore the clinical characteristics of hand arm vibration syndrome (HAVS) in a group tree fellers tropical environment. We examined all and selected control subjects logging camp central Sarawak for exposure presence HAVS symptoms utilizing vibrotactile perception threshold test (VPT) cold water provocation (CWP). None reported white finger. The significantly higher prevalence finger coldness as compared (OR=10.32, 95%CI=1.21–87.94). A lower skin temperature, longer...
Abstract Aims Neurogranin (NRGN) is a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. Recent studies suggest NRGN involved neuropsychiatric disorders, including schizophrenia, ADHD, and Alzheimer's disease. Previous behavioral Nrgn knockout ( KO) mice identified hyperactivity, deficits spatial learning, impaired sociability, decreased prepulse inhibition, which these recapitulate some symptoms disorders. To further validate KO as model we assessed...
Experimental animals, such as non-human primates (NHPs), mice, Zebrafish, and Drosophila, are frequently employed models to gain insights into human physiology pathology. In developmental neuroscience related research fields, information about the similarities of gene expression patterns between animal humans is vital choose what employ. Here, we aimed statistically compare changes in brains with those used field. The datasets that analyzed consist fold-changes P values animals various ages...
Abstract The CA1 area in the mammalian hippocampus is essential for spatial learning. Pyramidal cells are output neurons and their activities regulated by inhibition exerted a diversified population of interneurons. Lateral has been suggested as mechanism enabling reconfiguration pyramidal cell assembly activity observed during learning tasks rodents. However, lateral lacks overwhelming evidence reported other hippocampal areas such CA3 dentate gyrus. use genetically encoded voltage...
Abstract We have developed a novel device for the quantification of edematous morphology changes in acute brain slices. can also carry out real‐time monitoring detailed hippocampal cells. The we is based on infrared differential interference contrast microscopy (IR‐DIC) and custom‐made computerized image‐analysis system morphological dynamics cells slice preparations. applied coefficient variation (CV) light intensity IR‐DIC images to evaluate change dynamics. examined three kinds edema CA1...
Scanning small spots of laser light allows mapping synaptic circuits in brain slices from transgenic mice expressing channelrhodopsin-2 (ChR2). These photostimulate presynaptic neurons ChR2, while postsynaptic responses can be monitored that do not express ChR2. Correlating the location spot with amplitude response elicited at yields maps spatial organization circuits. This approach within minutes, which is several orders magnitude faster than achieved conventional paired...