Background Obturator-type hip dislocation is a rare condition in pediatric patients, with the simultaneous occurrence of an ipsilateral subtrochanteric femoral fracture being exceptionally uncommon. Although various treatment methods have been described for or fractures, managing these injuries remains challenging due to potential risk head avascular necrosis and concerns about fixation stability. Case presentation An 8-year-old boy sustained traumatic obturator following fall from height....

Calpastatin is an endogenous inhibitor of calpain, intracellular calcium-activated protease. It has been suggested to be involved in molecular mechanisms long-term plasticity and excitotoxic pathways. However, functions calpastatin vivo are still largely unknown. To examine the physiological roles calpastatin, we subjected calpastatin-knockout mice a comprehensive behavioral test battery. Calpastatin-knockout showed decreased locomotor activity under stressful environments, acoustic startle...

Valeriana amurensis, a perennial medicinal herb, has been widely used as anxiolytic, antidepressant, antispasmodic, and sedative in traditional Chinese medicines (TCMs). Moreover, it to treat dementia Mongolia preparations. In our previous study, we reported that AD-effective fraction of amurensis (AD-EFV) protective effect on Aβ-induced toxicity PC12 cells. Up now, however, the therapeutic Alzheimer disease (AD) not explored. This study was designed determine whether AD-EFV could improve...

Kai Xin San is a Chinese herbal formula composed of Radix Ginseng, Poria, Polygalae and Acorus Tatarinowii Rhizome. It has been used in China for many years treating amnesia. ameliorates amyloid-β (Aβ)-induced cognitive dysfunction neuroprotective vivo, but its precise mechanism remains unclear. Expression insulin-degrading enzyme (IDE), which degrades Aβ, strongly correlated with function. Here, we injected rats exogenous Aβ42 (200 μM, 5 μL) into the hippocampus subsequently administered...

Kaixin-San (KXS), a Chinese formula, was used to treat "amnesia," senile dementia in the modern world. This formula reported improve behavioral performances many animal models. study designed explore how KXS has improved amyloid-β (Aβ)-induced memory dysfunction mice. The mouse models were achieved through unilateral ventricle injection with Aβ42. effects of on improvement evaluated by step-down test. electrophysiological changes induced measured long-term potentiation (LTP) analysis...

Depressive symptoms are throughout our life, especially in the older population, sex hormones reduction link to a high risk of depression. In this study, we investigated whether bilateral orchiectomy (ORX) modifies mice behaviors and antidepressant drugs effects through tail suspension test (TST). We evaluated behavioral changes at 1 week, 2 weeks, month, up months after ORX. The behavior responses doxepin, fluoxetine, venlafaxine ORX were evaluated. No apparent difference was detected among...

This study aimed to investigate the mechanisms of Kai-Xin-San (KXS, a famous Chinese herbal decoction used treat amnesia) on degradation Aβ and further elucidate mechanism KXS Aβ-induced memory dysfunction. After pretreatment with (1.08 g/kg/day) for two weeks, Aβ42 (2 μL, 200 μM) was injected into rat hippocampus induce cognitive Morris water maze (MWM) test developed evaluate performance in rats. Hippocampal neurons were observed by histological staining using Hematoxylin-Eosin (HE)...

Depressive symptoms are very frequent over a lifetime, especially for women. Menopause is period of higher depressive vulnerability. There suggestive data that estrogen deficiency may increase the susceptibility depression. We studied whether bilateral ovariectomy (OVX) modifies mice behaviors and antidepressant drug effects through tail suspension test (TST). evaluated behavioral changes at 1 week, 2 weeks, up to months after OVX. The behavior responses doxepin, paroxetine, venlafaxine OVX...

Lung cancer is a severe health problem and threatens patient's quality of life. The metabolites present in biological systems are expected to be key mediators the changes these play an important role promoting health. Metabolomics can unravel global metabolic identify significant pathways involved disease development. However, lung still largely unknown. In study, we developed liquid chromatography coupled with tandem mass spectrometry method for biomarker discovery identification non-small...

Estrogen deficiency increases the incidence of female anxiety disorders; however, whether estrogen alters responses to anxiolytic drugs is unknown. We studied long-term deprivation (ovariectomy, OVX) changes behavior mice (buspirone, diazepam, and venlafaxine), using elevated plus maze (EPM) test. The percentages EPM open-arm time entries OVX decreased significantly compared control, sham 2 months after OVX. response buspirone increased in at 1 week, while diazepam months. Moreover, we found...

Previous small animal models for menopausal transition are mainly performed to mimic the biochemical changes of patients with transition. The psychological symptoms in often overlooked. To clinical situation, we developed a new mouse model transition: combination bilateral ovariectomy (OVX) and empty bottle stimulation (EBS). After OVX 21 days EBS, behavioral test pentobarbital-induced sleep were carried out. Then all animals euthanized further investigations. Compared control group, EBS...

Background Yin Huo Tang (YHT), a traditional Chinese herbal formula, is effectively used for the clinical treatment of menopause-like symptoms in China. This study aimed to investigate its efficacy on mice using behavioral tests and histopathological assessment, determine possible mechanism action based network pharmacology. Methods Liquid chromatography-mass spectrometry (LC-MS) technology was identify potential active ingredients YHT. In mice, were induced by combination bilateral...

Objective: To explore the mechanism underlying Kaixin-San (KXS) regulation of postsynaptic AMPA receptor (AMPAR) expression to mitigate toxic effects amyloid-β protein (Aβ). Methods: An animal model was established via intracerebroventricular injection Aβ1–42. The Morris water maze test conducted evaluate learning and memory, while electrophysiological recording assess hippocampal long-term potentiation (LTP). Western blotting used detect levels AMPAR its accessory proteins. Results: time...

Objective To discuss the expression of interleukin-32 (IL-32) in glioma and its regulatory mechanism. Methods RT-PCR Western blotting were used to detect mRNA protein levels IL-32 CHG-5 U251 cell lines vitro cultured for 3 d.And then, 10 ng/mL IL-1β, IL-4, IL-6, IL-10, IL-17, tumour necrosis factor alpha (TNF-α), TNF-β interferon-gamma(IFN-γ) put into 24 h,and was employed IL-32; meanwhile,RT-PCR also after treatment IL- 1 β,TNF-α IFN-γ different times,respectively. Results The cells...