A5100337172- Enzyme Catalysis and Immobilization
- Glycosylation and Glycoproteins Research
- Pharmacogenetics and Drug Metabolism
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolic Engineering and Bioproduction
- Microfluidic and Capillary Electrophoresis Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Microbial Natural Products and Biosynthesis
- Plant chemical constituents analysis
- Probiotics and Fermented Foods
- Enzyme Structure and Function
- Innovative Microfluidic and Catalytic Techniques Innovation
- Meat and Animal Product Quality
- Advanced X-ray and CT Imaging
- Animal Nutrition and Physiology
- Advanced oxidation water treatment
- Biochemical and biochemical processes
- Ion-surface interactions and analysis
- Medical Imaging Techniques and Applications
- Bioactive natural compounds
- Microwave-Assisted Synthesis and Applications
- Nanopore and Nanochannel Transport Studies
- Lysosomal Storage Disorders Research
- Sphingolipid Metabolism and Signaling
University of California, Davis
2021-2025
Shanghai Jiao Tong University
2024
Guizhou University
2024
Nanjing University of Science and Technology
2024
China Pharmaceutical University
2023
State Key Laboratory of Natural Medicine
2023
Jilin University
2011-2023
University of South Carolina
2020-2022
Liaoning University
2022
Shenzhen Institute of Information Technology
2022
Carbohydrates are biologically and medicinally important molecules that attracting growing attention to their synthesis applications. Unlike the biosynthetic processes for nucleic acids proteins, carbohydrate biosynthesis is not template-driven, more challenging, often leads product variations. In lieu of templates biosynthesis, we describe herein a new concept designing enzyme assembly synthetic maps (EASyMaps) as blueprints guide glycosyltransferase-dependent stepwise one-pot multienzyme...
RBM5 physically interacts DHX15, PRP19 and U2AF65 by anti bait coimmunoprecipitation (View interaction)
Protein N-glycosylation is a common post-translational modification that plays significant roles on the structure, property, and function of glycoproteins. Due to N-glycan heterogeneity naturally occurring glycoproteins, functions specific N-glycans particular glycoprotein are not always clear. Glycoprotein in vitro engineering using purified recombinant enzymes an attractive strategy produce glycoproteins with homogeneous N-glycoforms elucidate develop better therapeutics. Toward this goal,...
Pyran- and furanocoumarins are key representatives of tetrahydropyrans tetrahydrofurans, respectively, exhibiting diverse physiological medical bioactivities. However, the biosynthetic mechanisms for their core remain poorly understood. Here we combined multiomics analyses enzymes in Peucedanum praeruptorum vitro functional verification identified two types critical pyran furan ring biosynthesis plants. These included three distinct P. prenyltransferases (PpPT1–3) responsible prenylation...
Enzymes immobilized on cellulose-based supports provide important applications because they are convenient, economical, and stable. In this paper, we report the application of delignified bamboo as a novel scaffold for enzyme immobilization enzyme-based flow bioreactors, which can drastically improve catalytic reaction subsequent separation. The provides superior biobenign hierarchically structured platform that be conveniently derivatized to conjugate functional proteins with high loading...
Antler ossified tissue has been widely used for the extraction of bioactive peptides. In this study, collagen was prepared from antler via acetic acid and pepsin. Five different proteases were to hydrolyze hydrolysate treated by neutrase (collagen peptide named ACP) showed highest DPPH radical clearance rate. The process ACP optimized response surface methodology, optimal conditions as follows: a temperature 52 °C, pH 6.1, an enzyme concentration 3200 U/g, which resulted in maximum rate...
A process for extracting the antler tip peptides (ATPs) from Sika deer ( Cervus nippon ) tips was optimized using a method that combines single‐factor experiment and response surface methodology. The optimal conditions involved papain as hydrolase, an enzyme concentration of 5000 U/g, hydrolysis time 3.0 h, temperature 52.0°C, pH 7.10, substrate 14%. Under these conditions, clearance rate 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical 73.05 ± 1.49%, degree (DH) 19.43 1.06%. antioxidant...
Abstract Large‐scale synthesis of GM1, an important ganglioside in mammalian cells especially those the nervous system, is needed to explore its therapeutic potential. Biocatalytic production a promising platform for such purpose. We report herein development process engineering and glycosyltransferase improvement strategies advance chemoenzymatic total GM1. Firstly, new short route was developed chemical lactosylsphingosine from commercially available Garner's aldehyde. Secondly, two...
Benzylisoquinoline alkaloids (BIAs) are key plant metabolites that offer significant pharmacological benefits. While most naturally isolated BIAs identified as (S)-enantiomers, (R)-configured also abundant in specific species. However, the formation mechanism of (R)-enantiospecific remains largely unknown. Norcoclaurine synthase (NCS)-catalyzed Pictet–Spengler condensation is responsible for scaffold and establishing a unique chiral carbon center. Nevertheless, all NCSs hitherto were...
Abstract Polymer–protein core–shell nanoparticles have been explored for enzyme immobilization. This work reports on the development of functional polymeric micelles immobilizing His 6 ‐tagged cellulases with controlled spatial orientation enzymes, resulting in “artificial cellulosomes” effective cellulose hydrolysis. Poly(styrene)‐ b ‐poly(styrene‐ alt ‐maleic anhydride) was prepared through one‐pot reversible addition–fragmentation chain‐transfer polymerization and modified...
Nanopore technology has been employed as a powerful tool for DNA sequencing and analysis. To extend this method to peptide sequencing, necessary step is profile individual amino acids (AAs) through their nanopore stochastic signals, which remains great challenge because of the low signal-to-noise ratio unpredictable conformational changes AAs during translocation nanopores. We showed that combination an N-terminal derivatization strategy with could lead effective in situ differentiation AAs....
Gangliosides are sialic acid-containing glycosphingolipids that have been found in the cell membranes of all vertebrates. Their important biological functions contributed by both glycan and ceramide lipid components. GM3 is a major ganglioside precursor for many other more complex gangliosides. To obtain structurally diverse gangliosides containing various acid forms different fatty acyl chains low cost, an improved process was developed to chemically synthesize lactosyl sphingosine from...
Computed tomography angiography (CTA) has become the main imaging technique for cardiovascular diseases. Before performing transcatheter aortic valve intervention operation, segmenting images of sinus and nearby tissue from enhanced human heart is essential auxiliary diagnosis guiding doctors to make treatment plans. This paper proposes a nnU-Net (no-new-Net) framework based on deep learning (DL) methods segment aorta near in cardiac CTA images, verifies its accuracy effectiveness. A total...
The objective of this study was to investigate the correlation between dual-energy computed tomography (DECT)-based iodine quantitation and fluorine-18 fluorodeoxyglucose (F-FDG) positron emission (PET)/computed (CT) imaging for response evaluation lung cancers treatment.In prospective study, a total 32 pairs DECT F-FDG PET/CT acquired consecutively from 13 patients with primary or metastatic receiving either radiotherapy alone chemoradiotherapy were analyzed. Imaging examinations performed...
The stable N-acetyl analogues of biologically important 9-O-acetylated b-series gangliosides including 9NAc-GD3, 9NAc-GD2, 9NAc-GD1b, and 9NAc-GT1b were chemoenzymatically synthesized from a GM3 sphingosine. Two chemoenzymatic methods using either 6-azido-6-deoxy-N-acetylmannosamine (ManNAc6N3) as synthon or 6-acetamido-6-deoxy-N-acetylmannosamine (ManNAc6NAc) an enzymatic precursor for 9-acetamido-9-deoxy-N-acetylneuraminic acid (Neu5Ac9NAc) developed compared the synthesis 9NAc-GD3. latter...
Electrostatic accumulation is associated with almost all powder-conveying processes which could bring about electrostatic discharges. In most cases of industrial accidents, discharge proven to be the primary source ignition and explosion. Herein, a surface modification process polyaniline (PANI) proposed construct highly exothermic special powders, namely, HMX@PANI energetic composites, low charge for improving powder safety. Pure HMX are encapsulated within PANI-conductive polymer layer...
Abstract Cytochrome P450 OleT is a fatty acid decarboxylase that catalyzes the production of olefins with biofuel and synthetic applications. However, relatively sluggish catalytic efficiency enzyme limits its Here, we report application novel class benzene containing small molecules to improve activity. The UV‐Vis spectroscopy study molecular docking results confirmed high proximity heme group OleT. Up 6‐fold increase product yield has been achieved in molecule‐modulated enzymatic...
A novel N-oxygenase-coated core–shell nanoparticle was generated through the coassembly of poly(4-vinylpyridine) (P4VP) and arylamine N-oxygenase CmlI. The resulting enzyme-hybridized particles, P4VP-CmlI, showed excellent catalytic activities on oxidation two substrates, i.e., p-aminophenol (pAP) p-aminobenzoic acid (pABA), using a surrogate redox system or peroxide shunt as co-oxidants. In comparison with free enzyme, P4VP-CmlI particles exhibited significantly enhanced efficiency when...