A5041423154- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Metal complexes synthesis and properties
- Electrochemical Analysis and Applications
- Advanced Proteomics Techniques and Applications
- Organometallic Compounds Synthesis and Characterization
- Electrocatalysts for Energy Conversion
- Crystal structures of chemical compounds
- Lanthanide and Transition Metal Complexes
- Enzyme Structure and Function
- Trace Elements in Health
- Metabolomics and Mass Spectrometry Studies
- Crystallography and molecular interactions
- Mass Spectrometry Techniques and Applications
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Magnetism in coordination complexes
- Metal-Catalyzed Oxygenation Mechanisms
- COVID-19 Clinical Research Studies
- Chemical Synthesis and Analysis
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Prion Diseases and Protein Misfolding
- RNA and protein synthesis mechanisms
Charité - Universitätsmedizin Berlin
2021-2024
Freie Universität Berlin
2023
The Francis Crick Institute
2020-2023
Humboldt-Universität zu Berlin
2023
European X-Ray Free-Electron Laser
2019
SPring-8
2015-2019
Wroclaw Medical University
2012-2017
Université de Pau et des Pays de l'Adour
2012-2016
Centre National de la Recherche Scientifique
2012-2016
In-Q-Tel
2015
The dia-PASEF technology uses ion mobility separation to reduce signal interferences and increase sensitivity in proteomic experiments. Here we present a two-dimensional peak-picking algorithm generation of optimized spectral libraries, as well take advantage neural network-based processing data. Our computational platform boosts depth by up 83% compared previous work, is specifically beneficial for fast experiments those with low sample amounts. It quantifies over 5300 proteins single...
COVID-19 is highly variable in its clinical presentation, ranging from asymptomatic infection to severe organ damage and death. We characterized the time-dependent progression of disease 139 inpatients by measuring 86 accredited diagnostic parameters, such as blood cell counts enzyme activities, well untargeted plasma proteomes at 687 sampling points. report an initial spike a systemic inflammatory response, which gradually alleviated followed protein signature indicative tissue repair,...
Abstract Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is known to contain an active-site cysteine residue undergoing oxidation in response hydrogen peroxide, leading rapid inactivation of the enzyme. Here we show that human and mouse cells expressing a GAPDH mutant lacking this redox switch retain catalytic activity but are unable stimulate oxidative pentose phosphate pathway enhance their reductive capacity. Specifically, find anchorage-independent growth spheroids limited by elevation...
Functional genomic strategies have become fundamental for annotating gene function and regulatory networks. Here, we combined functional genomics with proteomics by quantifying protein abundances in a genome-scale knockout library Saccharomyces cerevisiae, using data-independent acquisition mass spectrometry. We find that global expression is driven complex interplay of (1) general biological properties, including translation rate, turnover, the formation complexes, growth genome...
Global healthcare systems are challenged by the COVID-19 pandemic. There is a need to optimize allocation of treatment and resources in intensive care, as clinically established risk assessments such SOFA APACHE II scores show only limited performance for predicting survival severely ill patients. Additional tools also needed monitor treatment, including experimental therapies clinical trials. Comprehensively capturing human physiology, we speculated that proteomics combination with new...
Abstract We present Slice-PASEF, a novel mass spectrometry technology based on trapped ion mobility separation of ions. Slice-PASEF allows to achieve the theoretical maximum MS/MS sensitivity and boosts proteomics low sample amounts. Leveraging we show, for first time, that comprehensive profiling single cell-level peptide amounts is possible using ultra-fast microflow chromatography general-purpose spectrometer, allowing quantification 1417 proteins from 200 picograms HeLa cell standard an...
Metabolism is deeply intertwined with aging. Effects of metabolic interventions on aging have been explained intracellular metabolism, growth control, and signaling. Studying chronological in yeast, we reveal a so far overlooked property that influences via the exchange metabolites. We observed metabolites exported by young cells are re-imported chronologically cells, resulting cross-generational interactions. Then, used self-establishing metabolically cooperating communities (SeMeCo) as...
Abstract Increased throughput in proteomic experiments can improve accessibility of platforms, reduce costs, and facilitate new approaches systems biology biomedical research. Here we propose combination analytical flow rate chromatography with ion mobility separation peptide ions, data‐independent acquisition, data analysis the DIA‐NN software suite, to achieve high‐quality from limited sample amounts, at a up 400 samples per day. For instance, when benchmarking our workflow using...
Two Cu<sup>II</sup> complexes with branched tetrapeptides undergo PCET and catalyse water oxidation, processes promoted by the presence of C-terminal histidine.
Abstract Genetically identical cells are known to differ in many physiological parameters such as growth rate and drug tolerance. Metabolic specialization is believed be a cause of phenotypic heterogeneity, but detection metabolically divergent subpopulations remains technically challenging. We developed proteomics-based technology, termed differential isotope labelling by amino acids (DILAC), that can detect producer consumer particular acid within an isogenic cell population monitoring...
Abstract The dia-PASEF technology exploits ion mobility separation for high-sensitivity analysis of complex proteomes. Here, we demonstrate neural network-based processing the data, which implement in DIA-NN software suite. Using spectral libraries generated with MSFragger-based FragPipe computational platform, raw data increases proteomic depth by up to 69% compared originally published workflow. For example, quantify over 5200 proteins from 10ng HeLa peptides separated a 95-minute nanoflow...
Abstract The reaction of Salen‐like Zn II and Ni precursors with carbacylamidophosphate lanthanide moieties yields six new types 3d–4f compounds. complexes were characterized by means 1 H, 31 P NMR IR spectroscopy, elemental analysis, ESI mass spectrometry, X‐ray diffraction analysis. Depending on the Schiff base ligands, ion adopts either a square‐planar or an octahedral geometry, whereas has tetragonal‐pyramidal geometry. coordination number lanthanides is nine ten. Hydrolytic activities...
The heterometallic [2 × 2] grid-type complex [Cu2Mn2(pop)4(OAc)4]·7H2O (1) has been selectively synthesized in a targeted one-pot reaction. Single-crystal X-ray analysis shows the expected structure with identical metal ions located at diagonal vertices of grid. Magnetochemical studies reveal that 1 ferrimagnetic spin ground state some admixture low-lying excited states.
The reactions of uranyl sources with 2-pyridyl ketoximes give a variety products; common structural motif in the complexes containing anionic ligands is η<sup>2</sup> coordination mode deprotonated oxime group and non-participation pyridyl nitrogen coordination.
Fatty acids are taken up by cells and incorporated into complex lipids such as neutral glycerophospholipids. Glycerophospholipids major constituents of cellular membranes. More than 1000 molecular species glycerophospholipids differ in their polar head groups fatty acid compositions. They related to functions diseases have been well analyzed mass spectrometry. However, intracellular imaging has not successful due insufficient resolution using conventional methods. Here, we developed a method...
Molecular design represents an exciting platform to refine mechanistic details of electrocatalytic water oxidation and explore new perspectives. In the growing number publications some general trends seem be outlined concerning operation mechanisms, with help experimental theoretical approaches that have been broadly applied in case bioinorganic systems. this review we focus on bio-inspired Cu-containing complexes are classified according proposed pathways related evidence, strongly linked...
The structure of the metabolic network is highly conserved, but we know little about its evolutionary origins. Key for explaining early evolution metabolism solving a chicken–egg dilemma, which describes that enzymes are made from very same molecules they produce. recent discovery several nonenzymatic reaction sequences topologically resemble central has provided experimental support “metabolism first” theory, in at least part extant emerged on basis reactions. But how could kick-start metal...
Abstract Increased throughput in proteomic experiments can improve accessibility of platforms, reduce costs and facilitate new approaches systems biology biomedical research. Here we propose Speedy-PASEF, a combination analytical flow rate chromatography with ion mobility separation peptide ions, data-independent acquisition data analysis the DIA-NN software suite, for conducting fast, high-quality that require only moderate sample amounts. For instance, using 500-μl/min 3-minute...
Summary Metal ions play crucial roles in cells, yet the broader impact of metal availability on biological networks remains underexplored. We generated genome-wide resources, systematically quantifying yeast cell growth, metallomic, proteomic, and genetic responses upon varying each its essential (Ca, Cu, Fe, K, Mg, Mn, Mo, Na, Zn), over several orders magnitude. find that deeply cellular networks, with 57.6% proteome, including most signalling pathways, responding. While response to is...
The homeostasis of metal ions, especially copper and zinc, is a major factor that may influence the prion diseases biological function protein (PrP). His-rich regions are basic sites for binding antioxidant activity PrP structures. Animal prion-like proteins contain also domains, their coordination chemistry provide better insight into biology structures related diseases. Herein, we report an equilibrium study on heteronuclear Zn2+−Cu2+ complexes with zrel-PrP fragments from zebrafish....
This work demonstrates the heterogenization of homogeneous water oxidation electrocatalysts in surface coatings produced by combining substances with a suitable polyelectrolyte. The i.e. Cu(ii)-branched peptide complexes involving 2,3-l-diaminopropionic acid junction unit are heterogenized building composite layers on indium-tin-oxide (ITO) electrode surface. Alternating deposition and poly(l-lysine) or poly(allylamine hydrochloride) were carried out presence phosphate pH range 7.5-10.5....
Triple-arm, His-rich branched peptide stabilizes 1 : Cu<sup>II</sup> complex forms by switching between N- and C-terminal His coordination upon changes in pH.
Abstract COVID-19 is highly variable in its clinical presentation, ranging from asymptomatic infection to severe organ damage and death. There an urgent need for predictive markers that can guide decision-making, inform about the effect of experimental therapies, point novel therapeutic targets. Here, we characterize time-dependent progression through different stages disease, by measuring 86 accredited diagnostic parameters plasma proteomes at 687 sampling points, a cohort 139 patients...