A5034699200- Iron Metabolism and Disorders
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Erythropoietin and Anemia Treatment
- Parathyroid Disorders and Treatments
- Mesenchymal stem cell research
- COVID-19 diagnosis using AI
- Folate and B Vitamins Research
- Hemoglobinopathies and Related Disorders
- Adipose Tissue and Metabolism
- Magnesium in Health and Disease
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Telomeres, Telomerase, and Senescence
- Wnt/β-catenin signaling in development and cancer
- Mycobacterium research and diagnosis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Liver Disease Diagnosis and Treatment
- German History and Society
- European history and politics
- Inflammasome and immune disorders
- Infectious Diseases and Tuberculosis
- Pectus Deformity Diagnosis and Treatment
- Blood transfusion and management
Christian Doppler Klinik
2020-2025
Universität Innsbruck
2019-2025
Innsbruck Medical University
2020-2025
Christian Doppler Laboratory for Thermoelectricity
2021-2024
University Hospital Innsbruck
2022
Universitätsklinikum Erlangen
2017
Helios Klinikum Emil von Behring
2012
Essen University Hospital
2008
COVID-19 is highly variable in its clinical presentation, ranging from asymptomatic infection to severe organ damage and death. We characterized the time-dependent progression of disease 139 inpatients by measuring 86 accredited diagnostic parameters, such as blood cell counts enzyme activities, well untargeted plasma proteomes at 687 sampling points. report an initial spike a systemic inflammatory response, which gradually alleviated followed protein signature indicative tissue repair,...
Global healthcare systems are challenged by the COVID-19 pandemic. There is a need to optimize allocation of treatment and resources in intensive care, as clinically established risk assessments such SOFA APACHE II scores show only limited performance for predicting survival severely ill patients. Additional tools also needed monitor treatment, including experimental therapies clinical trials. Comprehensively capturing human physiology, we speculated that proteomics combination with new...
The bone marrow (BM) is a major reservoir of resting memory T cells and long-lived plasma cells, capable providing protection against recurrent infections. Whether the age-related accumulation adipose tissue in BM affects functionality maintenance not well understood. For first time, we compare human femur (fMAT) subcutaneous white thigh (tsWAT) obtained from same donors. Therefore, used microarrays for comparative global gene expression analysis, employed assays to analyse parameters...
Abstract Background and Aims Haemochromatosis is characterized by progressive iron overload affecting the liver can cause cirrhosis hepatocellular carcinoma. Most haemochromatosis patients are homozygous for p.C282Y in HFE , but only a minority of individuals with this genotype will develop disease. The aim was to assess penetrance overload, fibrosis, carcinoma life expectancy. Methods A total 8839 from Austrian region Tyrol were genotyped variant between 1997 2021. Demographic, laboratory...
Patients with Crohn's disease are at increased risk for symptomatic nephrolithiasis. Stones in these patients most commonly composed of calcium oxalate monohydrate or mixed calcium-oxalate and calcium-phosphate. Precipitation both minerals depends on urinary pH, calcium, phosphate excretion. The present manuscript reports two bowel resection, whom the onset urolithiasis occurred after repeated infusions ferric carboxymaltose - a drug, which is known to cause hyperphosphaturia. study shows...
The single nucleotide polymorphism rs12979860 is associated with the production of IFNλ4, a type III interferon, which offers protection from viral infection via its proinflammatory properties. We investigated if genetically determined increase in IFNλ4 affects disease progression SARS-CoV-2. This prospective, single-center study involved critically ill SARS-CoV-2 patients admitted to intensive care unit. performed genotyping for and analyzed daily laboratory data. Genotype frequencies were...
We explore the status of quiescence, stemness and adipogenic differentiation capacity in adipose stem/progenitor cells (ASCs) ex vivo, immediately after isolation from human subcutaneous white tissue, by sorting stromal vascular fraction into cell-surface DLK1+/CD34−, DLK1+/CD34dim DLK1−/CD34+ cells. demonstrate that cells, only population exhibiting proliferative capacity, express vivo bonafide quiescence markers p21Cip1, p27Kip1 p57Kip2 but neither proliferation nor senescence marker...
Abstract The differentiation of adipose stem/progenitor cells (ASCs) into adipocytes contributes to tissue expansion in obesity. This process is regulated by numerous signalling pathways including MAPK signalling. In the present study, we show that weight loss (WL) interventions induce upregulation Sprouty1 ( SPRY1 ), a negative regulator signalling, human ASCs and elucidate role Sprouty1/MAPK interaction for adipogenic differentiation. We found protein levels are low proliferating ASCs,...
Abstract COVID-19 is highly variable in its clinical presentation, ranging from asymptomatic infection to severe organ damage and death. There an urgent need for predictive markers that can guide decision-making, inform about the effect of experimental therapies, point novel therapeutic targets. Here, we characterize time-dependent progression through different stages disease, by measuring 86 accredited diagnostic parameters plasma proteomes at 687 sampling points, a cohort 139 patients...
Abstract The role of Ras-Mitogen-activated protein kinase (MAPK) signaling in cellular aging is not precisely understood. Recently, we identified Sprouty1 (SPRY1) as a weight-loss target gene human adipose stem/progenitor cells (ASCs) and showed that important for proper regulation adipogenesis. In the present study, show loss-of-function by CRISPR/Cas9-mediated genome editing ASCs leads to hyper-activation MAPK senescence phenotype. knockout undergo an irreversible cell cycle arrest, become...
Interferon gamma release assays (IGRAs) are in vitro immunologic diagnostic tests used to identify Mycobacterium tuberculosis infection. They cannot differentiate between latent and active infections. The cutoff suggested by the manufacturer is 0.35 IU/mL for tuberculosis. As IGRA were recently approved differential diagnosis of tuberculosis, we assessed accuracy latest generation detection a low-incidence area Germany. Our consecutive case series includes 61 HIV negative, culture positive...
Hypophosphataemia is a common side-effect in patients with iron deficiency anaemia treated ferric carboxymaltose, which not class effect of all intravenous (IV) formulations. The report by Chu et al. shows that moderate and severe hypophosphataemia can even require IV supplementation phosphate unknown long-term consequences. Commentary on: Incidence predictors after carboxymaltose use-a 3-year experience from single institution Singapore. Br J Haematol 2023;202:1199-1204.
Abstract Global healthcare systems are challenged by the COVID-19 pandemic. There is a need to optimize allocation of treatment and resources in intensive care, as clinically established risk assessments such SOFA APACHE II scores show only limited performance for predicting survival severely ill patients. Comprehensively capturing host physiology, we speculated that proteomics combination with new data-driven analysis strategies could produce generation prognostic discriminators. We studied...
The CRISPR/Cas9 system is a powerful tool to generate specific loss-of-function phenotype by gene knockout (KO). However, this approach challenging in primary human cells. In technical report, we present reliable protocol achieve functional KO the genome of adipose stem/progenitor cells (ASCs). Using Sprouty1 (SPRY1) as model target for mediated KO, particularize procedure including selection sequences and employment appropriate lentiviral vectors obtain KO. efficiency mutate SPRY1...
Background Anemia is present in up to two-thirds of patients undergoing colorectal surgery mainly caused by iron deficiency and inflammation. As anemia associated with increased risk perioperative death, diagnosis treatment preoperative according etiology have been recommended. Objective The aim the study was assess if association between survival determined severity alone or also etiology. Methods To determine prevalence etiology, hematological parameters, C-reactive protein, ferritin...
Cardiovascular surgery might be followed by a complicated post-operative course with prolonged intensive medical care (PIMC) and extensive costs. The outcome after PIMC is unclear. Therefore the aim of this retrospective study to ascertain quality life survivors.