A5101641856- Pharmaceutical and Antibiotic Environmental Impacts
- Pesticide and Herbicide Environmental Studies
- Environmental Toxicology and Ecotoxicology
- Advanced Proteomics Techniques and Applications
- bioluminescence and chemiluminescence research
- Microbial bioremediation and biosurfactants
- Plant Stress Responses and Tolerance
- Water Quality and Pollution Assessment
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
- Soil Carbon and Nitrogen Dynamics
- Chemical Synthesis and Analysis
- Soil Geostatistics and Mapping
- Biotin and Related Studies
- Pesticide Exposure and Toxicity
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Water Treatment and Disinfection
- Phytase and its Applications
- Mass Spectrometry Techniques and Applications
- Soil and Water Nutrient Dynamics
- Plant nutrient uptake and metabolism
- Groundwater and Isotope Geochemistry
- Fecal contamination and water quality
Chinese Academy of Sciences
2011-2024
Guizhou University
2024
Beijing Forestry University
2024
Institute of Mechanics
2024
Institut Pasteur of Shanghai
2023
Institute of Microbiology
2023
Dalian Institute of Chemical Physics
2020-2022
Tobacco Research Institute
2017-2022
China Tobacco
2017-2022
Guangzhou Institute of Energy Conversion
2022
An effective chlorpyrifos-degrading bacterium (named strain YC-1) was isolated from the sludge of wastewater treating system an organophosphorus pesticides manufacturer. Based on results phenotypic features, phylogenetic similarity 16S rRNA gene sequences and BIOLOG test, YC-1 identified as genus Stenotrophomonas. The isolate utilized chlorpyrifos sole source carbon phosphorus for its growth hydrolyzed to 3,5,6-trichloro-2-pyridinol. Parathion, methyl parathion, fenitrothion also could be...
Abstract Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for first time using only N‐ and C‐terminal domains ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding INPNC–MPH was constructed used to target MPH onto a natural p ‐nitrophenol (PNP) degrader, putida JS444, overcoming potential substrate uptake limitation. Over 90% activity located cell determined by protease accessibility...
Methyl parathion (MP), a highly toxic organophosphorus pesticide, was widely used for agriculture crop protection. During the production of MP and process MP-containing wastewater treatment, can release into atmosphere will do great harm to adjacent communities. A consortium comprised an engineered microorganism natural p-nitrophenol (PNP) degrader assembled complete mineralization MP. We genetically Escherichia coli BL21 (DE3) enabling overexpression methyl hydrolase (MPH). In addition, we...
At present, Lpp−OmpA-mediated surface display has opened a new dimension in the development of whole-cell factories. Here, we report methyl parathion hydrolase (MPH) and enhanced green fluorescent protein (EGFP) fusions (60 kDa) by employing Lpp−OmpA chimera as an anchoring motif. A broad-host-range vector, pLOMG33, coding for Lpp−OmpA-MPH-GFP fusion was constructed targeting onto Escherichia coli. The localization demonstrated Western blot analysis, immunofluorescence microscopy, protease...
Organophosphorous hydrolase (OPH) was expressed onto the surface of a Stenotrophomonas species (LZ-1), capable simultaneously degrading 4-substituted phenols, using N- and C-terminal domains ice nucleation protein (INPNC) as an anchoring motif for first time. The engineered strain LZ-1 could degrade p-nitrophenyl-substituted organophosphates well their hydrolytic product, PNP, rapidly. Especially, addition 4-CP (below 0.8 mM) significantly accelerated complete degradation above (47.1, 34.0,...
Surface display of the active proteins on living cells has enormous potential in degradation numerous toxic compounds. Here, we report codisplay organophosphorus hydrolase (OPH) and enhanced green fluorescent protein (GFP) cell surface Escherichia coli by use truncated ice nucleation (INPNC) Lpp-OmpA fusion systems. The localization both INPNC-OPH Lpp-OmpA-GFP was demonstrated Western blot analysis, immunofluorescence microscopy, a protease accessibility experiment. Anchorage GFP OPH outer...
A copper-resistant strain (09906) of Pseudomonas fluorescens that was isolated from a citrus grove soil is being investigated as biological control agent for Phytophthora root rot. Since soils in California are often contaminated with copper many years fungicide applications, the role resistance survival 09906 investigated. Three copper-sensitive Tn5 mutants were obtained insertions different chromosomal DNA regions. These not region hybridized operon (cop) cloned syringae. mutant survived...
Experiments were designed to determine the effects of different chemical inhibitors lysozyme and peptidases on rumen protozoa associated prokaryotes, in vitro fermentation using Entodinium caudatum as a model protozoan species.Imidazole (a inhibitor), phenylmethylsulphonyl fluoride (PMSF, serine peptidase inhibitor) iodoacetamide (IOD, cysteine evaluated both individually two- three-way combinations E. monocultures with respect their ability inhibit effect feed digestion, microbiota. All...
Zelkova schneideriana Hand.-Mazz is a valuable ornamental tree and timber source, whose seedling breeding large-scale cultivation are restricted by low seed germination rates. The regulatory mechanisms underlying establishment in Z. remain unknown. This study conducted metabolomic transcriptomic analyses of schneideriana. Regular expression genes metabolite levels has been observed plant hormone signal transduction, starch sucrose metabolism, linoleic acid phenylpropanoid biosynthesis....
Organophosphorus pesticides (OPs) cause serious environmental problems, and bioremediation using bacterial enzymes may provide an efficient economical method for their detoxification. Green fluorescent protein (GFP) is a stable easily detectable marker in monitoring genetically engineered microorganisms (GEMs) the environment. In our research, methyl parathion hydrolase gene (mpd) enhanced green (egfp) were successfully coexpressed pETDuet vector E. coli BL21 (DE3). The coexpression of (MPH)...
The uptake of organophosphates (OPs) is a rate-limiting factor in whole-cell biocatalysis systems. Here, we report the periplasmic secretion methyl parathion hydrolase (MPH) by employing twin-arginine translocation (Tat) pathway Escherichia coli. signal peptide trimethylamine N-oxide reductase (TorA) from E. coli was used for exporting MPH to periplasm coli, alleviating substrate limitation. A expression vector, pUTM18, coding TorA−MPH constructed, and functionality were demonstrated cell...
Although many members encoding different ammonium- and nitrate-transporters (AMTs, NRTs) were identified functionally characterized from several plant species, little is known about molecular components for NH4+- NO3- acquisition/transport in tobacco, which often used as a model biological studies besides its agricultural industrial interest. We reported here the first identification tobacco (Nicotiana tabacum) of nine AMTs four NRTs, are respectively divided into (AMT1/2/3/4) two (NRT1/2)...