A5070544806- Postharvest Quality and Shelf Life Management
- Analytical chemistry methods development
- Plant Physiology and Cultivation Studies
- Horticultural and Viticultural Research
- Mercury impact and mitigation studies
- Heavy metals in environment
- Electrochemical Analysis and Applications
- Fermentation and Sensory Analysis
- Heavy Metal Exposure and Toxicity
- Pesticide Residue Analysis and Safety
- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- Plant biochemistry and biosynthesis
- Environmental Toxicology and Ecotoxicology
- Polysaccharides and Plant Cell Walls
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Plant Reproductive Biology
- Plant Surface Properties and Treatments
- Water Quality Monitoring and Analysis
- Trace Elements in Health
- Toxic Organic Pollutants Impact
- Marine animal studies overview
- Heavy Metals in Plants
- Iron Metabolism and Disorders
University of Chile
2017-2025
Universidad Andrés Bello
2011-2021
University of Bologna
2017
Pontifical Catholic University of Rio de Janeiro
2005-2014
Universidade Federal do Estado do Rio de Janeiro
2001-2012
Cell Biotech (South Korea)
2012
Millennium Engineering and Integration (United States)
2008-2011
National Council for Scientific and Technological Development
2010
Instituto de Investigaciones Agropecuarias
2005-2009
Universidade Federal do Rio de Janeiro
1986-2007
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family.Optimized protocols RNA isolation are necessary to further advance studies in this species such that functional genomics analyses may be performed.Here we present an optimized protocol rapidly efficiently purify high quality total from peach fruits (Prunus persica).Isolating high-quality fruit tissue is often difficult due large quantities of polysaccharides polyphenolic compounds accumulate...
Plant defensins, components of the plant innate immune system, are cationic cysteine-rich antifungal peptides. Evidence from literature [Thevissen, K., et al. (2003) Peptides 24, 1705−1712] has demonstrated that patches fungi membrane containing mannosyldiinositolphosphorylceramide and glucosylceramides selective binding sites for defensins isolated Dahlia merckii Raphanus sativus, respectively. Whether interact directly or indirectly with fungus intracellular targets is unknown. To identify...
Abstract Background Peach fruit undergoes a rapid softening process that involves number of metabolic changes. Storing at low temperatures has been widely used to extend its postharvest life. However, this leads undesired changes, such as mealiness and browning, which affect the quality fruit. In study, 2-D DIGE approach was designed screen for differentially accumulated proteins in peach during normal well under conditions led chilling injury. Results The analysis allowed us identify 43...
Cutting leaves of Romaine lettuce (Lactuca sativa L. cv. Longifolia) produces a wound signal that induces the synthesis phenylalanine ammonia lyase (PAL, EC 4.3.1.5) and accumulation phenolic compounds in cells up to 2 cm from site injury, tissue browning near injury. The response within head putative chemical signals [abscisic acid (ABA), jasmonate (JA) methyl (MeJA)] differed significantly with leaf age. Exposure harvested heads ABA, JA, MeJA, or salicylic (SA) did not induce changes PAL...
Volatile species of Ag, Au, Cu, Zn, Cd, and As were generated at room temperature by the addition sodium tetrahydroborate(III) to an acidified solution analytes. The vapor-phase rapidly transported a heated quartz tube atomizer (QTA) for detection atomic absorption spectrometry. A univariate approach was used achieve optimized conditions derive analytical figures merit. analytes are released from as molecular (likely metal hydrides) (partially) atomized in QTA under nonoptimized conditions....
This paper intends to evaluate the uptake of cadmium ions from aqueous solution by 21 hydroxyapatite samples which have been synthesized in different conditions. It has determined that variation on sorption capacity is neither related sample solubility nor Ca/P molar ratio. Cd2+ controlled BET surface area, shows a direct dependence crystallite dimensions. The pore distribution presented modes at 1000 and 60 000 Å, corresponding intracrystallite voids between agglomerate these crystallites,...
Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods cold storage. This commercial importance since shipping to distant markets storage before selling require low temperature. However, knowledge about the molecular basis peach woolliness still incomplete. To address this issue, nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from ripe cDNA library was developed used. Gene expression...
Cold storage is used to inhibit peach fruit ripening during shipment distant markets. However, this cold can negatively affect the quality of when it ripened, resulting in disorders such as wooliness, browning or leathering. In order understand individual and combined biological effects that factors have on quality, we taken a comparative EST transcript profiling approach identify genes are differentially expressed response these factors.We sequenced 50,625 Expressed Sequence Tags (ESTs)...
Phenylalanine ammonia‐lyase (PAL) catalyses the first step controlling rate of phenylpropanoid metabolism. Wounding is a ubiquitous stress in nature and harvesting preparation fruits vegetables that induces an increase PAL activity, accumulation phenolic compounds subsequent tissue browning. A wound‐inducible gene ( LsPAL1 ) was isolated from Romaine lettuce by RT‐PCR. The putative protein encoded similar to predictive polypeptides sequences for other PALs. kinetics mRNA those induced enzyme...
Wounding lettuce ( Lactuca sativa L., var. Longifolia) leaves induced an eight‐fold increase in the activity of phenylalanine ammonia‐lyase (PAL; EC 4.3.1.5), and subsequent accumulation phenolic compounds tissue browning at 10°C. PAL is a key enzyme synthesis compounds. A cDNA was previously isolated by reverse‐transcription PCR using total RNA from wounded leaves. gel blots showed that maximum both mRNA occurred 24 h after wounding. 2‐min heat shock 45°C administered within 5 min wounding...