A5081941934- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Glutathione Transferases and Polymorphisms
- Diagnosis and treatment of tuberculosis
- Carcinogens and Genotoxicity Assessment
- Chemical Reactions and Isotopes
- Respiratory Support and Mechanisms
- Pharmacogenetics and Drug Metabolism
- Sepsis Diagnosis and Treatment
- Pneumonia and Respiratory Infections
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Helicobacter pylori-related gastroenterology studies
- DNA and Nucleic Acid Chemistry
- interferon and immune responses
- COVID-19 Clinical Research Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advanced biosensing and bioanalysis techniques
- Respiratory viral infections research
- Biochemical and Molecular Research
- Nosocomial Infections in ICU
- Genetic Associations and Epidemiology
- Adrenal Hormones and Disorders
- Effects of Radiation Exposure
- Inflammatory Biomarkers in Disease Prognosis
Vavilov Institute of General Genetics
2010-2024
St Petersburg University
2023
Lomonosov Moscow State University
2010
Rare variants affecting host defense against pathogens may be involved in COVID-19 severity, but most rare are not expected to have a major impact on the course of COVID-19. We hypothesized that accumulation weak effects many functional throughout exome contribute overall risk patients with severe disease. This assumption is consistent omnigenic model relationship between genetic and phenotypic variation complex traits, according which association signals tend spread across genome through...
The aim of the study was to investigate serial changes in inflammatory indices derived from blood cell counts and C-reactive protein (CRP) levels COVID-19 patients with good poor outcomes. We retrospectively analyzed 169 patients. Comparative analyses were performed on first last days a hospital stay or death serially day 1 30 symptom onset. On admission, non-survivors had higher CRP lymphocytes ratio (CLR) multi-inflammatory index (MII) values than survivors, while at time discharge/death,...
. The Mycobacterium tuberculosis Beijing genotype is globally spread lineage with important medical properties that however vary among its subtypes. M. 14717-15-cluster was recently discovered as both multidrug-resistant, hypervirulent, and highly-lethal strain circulating in the Far Eastern region of Russia. Here, we aimed to analyze pathogenomic features phylogeographic pattern.
Brain tumors are the common site for solid in childhood. Very few studies have investigated genes with low penetrance relation to pediatric brain tumor (pBT) development. do occur more frequently males compared females regardless of age, histology, or region world. Taken into account these facts, we designed a study aimed analyse contribution some genetic factors pBP and females. Patients glial embryonic (160 males, 124 females) healthy controls (277 187 were included study. All subjects...
Multiple myeloma (MM) is the second most common hematological malignancy, which remains incurable despite recent advances in treatment strategies. Like other forms of cancer, MM characterized by genomic instability, caused defects DNA repair. Along with mutations repair genes and genotoxic drugs used to treat MM, non-canonical secondary structures (four-stranded G-quadruplex structures) can affect accumulation somatic chromosomal abnormalities tumor cells patients. Here, we tested hypothesis...
Host genetic variations may influence a changing profile of biochemical markers and outcome in patients with trauma/injury. The objective this study was to assess clinical associations single nucleotide polymorphisms (SNPs) the genes cytokines critically ill patients. A total 430 were genotyped for SNPs pro- (IL1B, IL6, IL8) anti-inflammatory (IL4, IL10, IL13) cytokines. main end-points sepsis, mortality adult respiratory distress syndrome (ARDS). We evaluated dynamic levels bilirubin, blood...
This study presents the results of research on DNA polymorphism in children with malignant brain tumors (172 patients, 183 control group). Genotyping was performed using an allele-specific tetraprimer reaction for genes first (CYP1A1 (2 sites)) and second phases xenobiotic detoxication (GSTM1, GSTT1, GSTP1, GSTM3), repair XRCC1, XPD sites), OGG1, as well NOS1 MTHFR. The increased risk disease is associated a minor variant CYP1A1 (606G) (p = 0.009; OR 1.50) deletion 0.013, 1.96). Maximum...
We performed synthesis of new nitrofuranyl amides and investigated their anti-TB activity primary genetic response mycobacteria through whole-genome sequencing (WGS) spontaneous resistant mutants. The in vitro was assessed on reference strain Mycobacterium tuberculosis H37Rv. most active compound 11 used for selection same mutations six genes were detected bacterial cultures grown under increased concentrations (2×, 4×, 8× MIC). mutant positions presented as mixed wild type alleles while...
With the aim of developing genetic tests for elevated and reduced radiation sensitivity, authors studied correlations between various genotypes frequencies spontaneous radiation-induced chromosome aberrations in human lymphocytes. Cytogenetic analysis genotyping (19 sites detoxification DNA repair genes) were carried out a group cleanup workers Chernobyl nuclear power plant accident (83 people) uniform control volunteers (97 people). In both cases, type higher carriers minor alleles gene XPD...
Genetic predisposition partially accounts for the clinical variability of course an infectious process. A total 750 people, including 419 (81.1%) male patients aged 42.9±0.9 years, admitted to clinics V. A. Negovsky Research Institute General Reanimatology (Moscow, Russia), were genotyped establish influence genetic factors on their susceptibility critical conditions. Materials and methods. Tetra-primer allele-specific polymerase chain reaction was used investigate single-nucleotide...
Pneumonia is an acute infectious disease with high morbidity and mortality rates. Pneumonia's development, severity outcome depend on age, comorbidities the host immune response. In this study, we combined theoretical experimental investigations to characterize pneumonia its as well assess response measured by TREC/KREC levels in patients pneumonia. The study was carried out using Columbia Open Health Data (COHD) resource, which provides access clinical concept prevalence co-occurrence from...
This study aimed to determine phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis strains from children with (TB) in China Russia, two high-burden countries for multi/extensively-drug resistant (MDR/XDR) TB. Whole-genome sequencing data M. isolates (n = 137) Russia 60) were analyzed phylogenetic markers drug-resistance mutations, followed by comparison susceptibility data. The Beijing genotype was detected 126 Chinese 50 Russian isolates. Euro-American lineage 10...
Aim. To develop a method for detecting Mycobacterium tuberculosis strains belonging to highly lethal, hypervirulent, multidrug-resistant Beijing 14717-15-cluster be applied screen M. retrospective collections obtained from various regions of Russia. The collection included DNA samples during population studies. Spoligotyping and genotyping 24 variable number tandem repeats loci were performed according standard protocols. Phylogenetic analysis was based on the whole genome sequencing data....
The results of the associative study predisposition to an elevated somatic mutagenesis in human lymphocytes determined by test TCR mutant cells (CD3-CD4+phenotype) for 251 females are presented. presence minor alleles polymorphic sites CYP1A1 gene, which increase enzyme activity, correlated with increasing spontaneous frequency cells. analysis gaplotypes locus (3 sites) showed that a gaplotype CG T3801C-T606G, had strong effect on parameters mutation at around 10%, has maximum prognostic...