A5075918764- Steroid Chemistry and Biochemistry
- Pharmacogenetics and Drug Metabolism
- Microbial Fuel Cells and Bioremediation
- Electrochemical sensors and biosensors
- Microbial Metabolic Engineering and Bioproduction
- Hormonal Regulation and Hypertension
- Algal biology and biofuel production
- Supercapacitor Materials and Fabrication
- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Plant-Microbe Interactions and Immunity
- Crystallization and Solubility Studies
- Electrochemical Analysis and Applications
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Fermentation and Sensory Analysis
- Nematode management and characterization studies
- Molecular Biology Techniques and Applications
- Evolution and Genetic Dynamics
- Chemical Reactions and Isotopes
- RNA and protein synthesis mechanisms
- Phytochemistry and Bioactive Compounds
- Evolutionary Game Theory and Cooperation
- X-ray Diffraction in Crystallography
- Genomics and Phylogenetic Studies
Tianjin University of Science and Technology
2016-2025
Tianjin Economic-Technological Development Area
2017-2020
Nankai University
2018-2020
Institute for Fermentation
2020
Tianjin Medical University
2019-2020
Lanzhou Veterinary Research Institute
2015
Chinese Academy of Agricultural Sciences
2015
Chongqing Three Gorges University
2011
Ministry of Education of the People's Republic of China
2010
University of Shanghai for Science and Technology
2008
In recent years, the bacteriophage Φ29 (Phi29) DNA polymerase has garnered increasing attention due to its high-fidelity amplification capacity at constant temperatures. To advance industrial application of this type isothermal polymerases, study mined and characterized new enzymes from microbial metagenome based on known Phi29 sequence. The results revealed that a enzyme, Php29 polymerase, was identified in with plants as hosts. This enzyme exhibited higher strand displacement activity,...
Improvement of natamycin production by Streptomyces gilvosporeus ATCC 13326 was performed recursive protoplast fusion in a genome-shuffling format. After four rounds genome shuffling, the best producer, GS 4-21, with genetic stability obtained and its reached 4.69 ± 0.05 g/L shaking flask after 96 h cultivation, which increased 97.1% 379% comparison highest parental strain pop-72Ar07 initial 13326, respectively. Compared recombinant 4-21 presented higher polymorphism. Fifty-four proteins...
Polyphenols extracted from Shanxi-aged vinegar (SAVEP) can alleviate oxidative stress and inflammatory caused by high-fat diets, improving intestinal microbial disorders. SAVEP may be a novel treatment of the metabolic syndrome.
Androstenedione (AD) is an important steroid medicine intermediate that obtained via the degradation of phytosterols by mycobacteria. The production process AD mainly phytosterol aliphatic side chain, which accompanied propionyl CoA. Excessive accumulation intracellular propionyl-CoA produces a toxic effect in mycobacteria, restricts improvement efficiency. 2-methylcitrate cycle pathway (MCC) plays significant role detoxification bacterial. MCC on biotransformation mycobacteria has not been...
Oligonucleotides represent a class of shorter DNA or RNA nucleic acid polymers extensively applied in the biomedical field. Despite progress detecting and analyzing oligonucleotides, high-throughput analysis samples remains challenging. In this work, method for oligonucleotide was developed based on acoustic droplet ejection-open port interface-mass spectrometry (ADE-OPI-MS) technology. This approach to determine enzymatic activity terminal deoxynucleotide transferase (TdT) synthesis, with...