A5008191157- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Cytomegalovirus and herpesvirus research
- Bacterial Genetics and Biotechnology
- Innovation and Socioeconomic Development
- Streptococcal Infections and Treatments
- Insect symbiosis and bacterial influences
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Microbial infections and disease research
- Neonatal and Maternal Infections
- Vibrio bacteria research studies
- Evolution and Genetic Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Plant Virus Research Studies
- Biochemical and Molecular Research
- Bacterial biofilms and quorum sensing
- Microbial Metabolites in Food Biotechnology
- RNA regulation and disease
- Peptidase Inhibition and Analysis
- Bacterial Infections and Vaccines
- Chemotherapy-related skin toxicity
DuPont (France)
2012-2021
DuPont (United States)
2012-2013
Vilnius University
2011
Université Laval
2007
Rush University Medical Center
1995
Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes most Bacteria and Archaea thought to be involved in resistance bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition particular modified phage-resistance phenotype cell. Thus, CRISPR, together with associated cas genes, provided against phages, specificity is determined by spacer-phage sequence similarity.
Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide adaptive immunity against viruses and plasmids in bacteria archaea. The silencing of invading nucleic acids is executed by ribonucleoprotein complexes preloaded with small, interfering CRISPR RNAs (crRNAs) that act as guides for targeting degradation foreign acid. Here, we demonstrate the Cas9–crRNA complex Streptococcus thermophilus CRISPR3/Cas system introduces vitro a double-strand...
ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated genes are linked to a mechanism of acquired resistance against bacteriophages. Bacteria can integrate stretches phage-derived sequences (spacers) within CRISPR loci become phage resistant. In this study, we further characterized the efficiency CRISPR1 as in Streptococcus thermophilus . First, show that is distinct from previously known defense systems effective two main groups S. phages. Analyses...
The CRISPR/Cas adaptive immune system provides resistance against phages and plasmids in Archaea Bacteria.CRISPR loci integrate short DNA sequences from invading genetic elements that provide small RNA-mediated interference subsequent exposure to matching nucleic acids.In Streptococcus thermophilus, it was previously shown the CRISPR1/Cas can immunity by integrating novel spacers following these foreign subsequently direct specific cleavage of invasive homologous sequences.Here, we show S....
ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR) are hypervariable loci widely distributed in prokaryotes that provide acquired immunity against foreign genetic elements. Here, we characterize a novel Streptococcus thermophilus locus, CRISPR3, and experimentally demonstrate its ability to integrate spacers response bacteriophage. Also, analyze CRISPR diversity activity across three distinct several S. strains. We show both cas genes locus specific functionally...
The Cas9-crRNA complex of the Streptococcus thermophilus DGCC7710 CRISPR3-Cas system functions as an RNA-guided endonuclease with crRNA-directed target sequence recognition and protein-mediated DNA cleavage. We show here that additional RNA molecule, tracrRNA (trans-activating CRISPR RNA), co-purifies Cas9 protein isolated from heterologous E. coli strain carrying S. system. provide experimental evidence is required for Cas9-mediated interference both in vitro vivo. specifically promotes...
Abstract CRISPR-Cas systems are bacterial anti-viral systems, and viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs also fine-tune the activity of CRISPR-based genome-editing tools. While prevalent in phages capable lying dormant a CRISPR-carrying host, their orthologs have been observed only infrequently virulent phages. Here we identify AcrIIA6, an Acr encoded 33% Streptococcus thermophilus phage genomes. The X-ray structure AcrIIA6 displays...
In streptococcal species, the key step of competence development is transcriptional induction comX, which encodes alternative sigma factor sigma(X), positively regulates genes necessary for DNA transformation. Streptococcus species belonging to mitis and mutans groups, comX relies on activation a three-component system consisting secreted pheromone, histidine kinase, response regulator. thermophilus, salivarius group, oligopeptide transporter Ami essential expression under...
Well-studied innate immune systems exist throughout bacteria and archaea, but a more recently discovered genomic locus may offer prokaryotes surprising immunological adaptability. Mediated by cassette-like termed Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), the microbial adaptive system differs from its eukaryotic analogues incorporating new immunities unidirectionally. CRISPR thus stores genomically recoverable timelines of virus-host coevolution in natural organisms...
ABSTRACT Bifidobacteria are important members of the human gut flora, especially in infants. Comparative genomic analysis two Bifidobacterium animalis subsp. lactis strains revealed evolution by internal deletion consecutive spacer-repeat units within a novel clustered regularly interspaced short palindromic repeat locus, which represented largest differential content between genomes. Additionally, 47 single nucleotide polymorphisms were identified, consisting primarily nonsynonymous...
The broad ecological distribution of L. casei makes it an insightful subject for research on genome evolution and lifestyle adaptation. To explore evolutionary mechanisms that determine genomic diversity casei, we performed comparative analysis 17 genomes representing strains collected from dairy, plant, human sources. Differences in inventory revealed open pan-genome comprised 1,715 core 4,220 accessory genes. Extrapolation data indicates has a supragenome approximately 3.2 times larger...
Interactions between bacteria and their coexisting phage populations impact evolution can strongly influence biogeochemical processes in natural ecosystems. Periodically, mutation or migration results exposure of a host to which it has no immunity; alternatively, may be exposed cannot infect. To explore the by coexisting, co-evolving hosts establish, we cultured Streptococcus thermophilus DGCC7710 with 2972 tracked CRISPR (clustered regularly interspaced short palindromic repeats)...
The CRISPR/Cas system, comprised of clustered regularly interspaced short palindromic repeats along with their associated (Cas) proteins, protects bacteria and archaea from viral predation invading nucleic acids. While the mechanism action for this acquired immunity is currently under investigation, response Cas protein expression to phage infection has yet be elucidated. In study, we employed shotgun proteomics measure global proteome in a model system studying S. thermophilus DGCC7710...