A5050524015- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Indoor Air Quality and Microbial Exposure
- Plant Virus Research Studies
- Polyamine Metabolism and Applications
- Innovation and Socioeconomic Development
- Microbial infections and disease research
- Agricultural and Food Production Studies
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Viral Infections and Immunology Research
Université de Bretagne Occidentale
2019
Université Laval
2009-2018
Chr. Hansen (Denmark)
2017
Instituto de Productos Lácteos de Asturias
2006-2008
Consejo Superior de Investigaciones Científicas
2008
Streptococcus thermophilus, similar to other Bacteria and Archaea, has developed defense mechanisms protect cells against invasion by foreign nucleic acids, such as virus infections plasmid transformations. One system recently described in these organisms is the CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats loci coupled CRISPR-associated genes). Two S. thermophilus systems, CRISPR1-Cas CRISPR3-Cas, have been shown actively block phage infection. The interferes...
Abstract Three cos -type virulent Streptococcus thermophilus phages were isolated from failed mozzarella production in Uruguay. Genome analyses showed that these are similar to those elsewhere around the world. The CRISPR1 and CRISPR3 arrays of three S. host strains Uruguay also characterized similarities noted with previously described model SMQ-301, LMD-9 DGCC7710. Spontaneous bacteriophage-insensitive mutants (BIMs) obtained after challenging phage-sensitive wild-type strain Uy02 phage...
Biogenic amines (BA)-nitrogenous compounds of low molecular weight- are the result metabolism certain amino acids. They biologically present in all living organisms and play essential physiological roles. However, their accumulation foodstuffs due to metabolic activity microorganisms represents a toxicological risk. Containing such microorganisms, with an abundance precursor substrate acids, fermented foods general, cheeses particular, provide ideal matrix for these toxic compounds....
The fermentation of milk by Streptococcus thermophilus is a widespread industrial process that susceptible to bacteriophage attack. In this work, preventive fast real-time PCR method for the detection, quantification, and identification types S. phages in 30 min described.
CRISPR-Cas defends microbial cells against invading nucleic acids including viral genomes. Recent studies have shown that type III-A systems target both RNA and DNA in a transcription-dependent manner. We previously found system on conjugative plasmid Lactococcus lactis which provided resistance virulent phages of the Siphoviridae family. Its naturally occurring spacers are oriented to generate crRNAs complementary phage mRNA, suggesting targeting. Here, we show only constructs whose produce...
Summary Lactococcus lactis , a Gram‐positive bacterium widely used by the dairy industry, is subject to infection diverse population of virulent phages, predominantly those 936 group, including siphovirus phage p2. Confronted with negative impact on milk fermentation, study biology lactococcal provides insight from applied and fundamental perspectives. We decided characterize product orf34 gene lactococcus p2, which was considered as candidate single‐stranded DNA binding protein (SSB) due...