A5013421430- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Advanced Chemical Sensor Technologies
- Biochemical effects in animals
- Effects and risks of endocrine disrupting chemicals
- Bacteriophages and microbial interactions
- Microplastics and Plastic Pollution
- RNA and protein synthesis mechanisms
- Tissue Engineering and Regenerative Medicine
- Neurobiology and Insect Physiology Research
- Veterinary Equine Medical Research
- Wound Healing and Treatments
- Pesticide and Herbicide Environmental Studies
- Free Radicals and Antioxidants
- Tendon Structure and Treatment
- Insect and Pesticide Research
- Reproductive Biology and Fertility
- Platelet Disorders and Treatments
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Liver Disease Diagnosis and Treatment
- Meat and Animal Product Quality
- Estrogen and related hormone effects
University of Parma
2016-2025
Associazione Italiana per l'Agricoltura Biologica
2005
Bioénergétique et Ingénierie des Protéines
1998
Architecture et Fonction des Macromolécules Biologiques
1998
Centre National de la Recherche Scientifique
1998
Institute of Molecular Biology and Pathology
1987
The X-ray structure of the porcine odorant binding protein (OBPp) was determined at 2.25 Å resolution. This lipocalin is a monomer and devoid naturally occurring bound ligand, contrary to what observed in case bovine OBP [Tegoni, M., et al. (1996) Nat. Struct. Biol. 3, 863−867; Bianchet, M. A., 934−939]. In this latter protein, dimer without any disulfide bridges, domain swapping found occur between β- α-domains. A single Gly (121) insertion OBPp when it compared OBPb, which may prevent from...
Overstrain tendonitis are common pathologies in the sport horses. Therapeutic approaches to tendon healing do not always result a satisfactory anatomical and functional repair, healed is often characterized by impairment high risk of reinjury. Recently, mesenchymal stem cells (MSCs) platelet rich plasma (PRP) have been proposed as novel therapeutic treatments improve repair process. MSCs multipotent, easy culture being originated from adult donors pose ethical issues. To date, autologous...
Bovine odorant-binding protein (bOBP) is a dimeric lipocalin present in large amounts the respiratory and olfactory nasal mucosa. The structure of bOBP refined at 2.0-Å resolution revealed an elongated volume electron density inside each buried cavity, indicating presence one (or several) naturally occurring copurified ligand(s) (Tegoni et al. (1996)Nat. Struct. Biol. 3, 863–867; Bianchet al.(1996) Nat. 934–939). In work, by combining mass spectrometry, x-ray crystallography (1.8-Å...
Laminitis, a highly debilitating disease of the foot in ungulates, is characterized by pathological changes complex lamellar structures that maintain appendicular skeleton within hoof. Laminitis multifactorial involves perturbation vascular, hematological, and inflammatory homeostasis foot. Interestingly, pathogenesis resembles what observed metabolic syndromes sepsis-induced organ failure humans animals. We hypothesized local administration mesenchymal stem cells (MSCs) platelet-rich plasma...
Extensive full-thickness skin wounds are quite common in domestic animals. In these report, following the failure of reconstructive surgery, adipose tissue-derived mesenchymal stem cells, and platelet-rich plasma were successfully used a dog to improve speed quality tissue healing, avoiding suffering, debilitating effects.
As revealed by the X-ray structure, bovine odorant-binding protein (OBPb) is a domain swapped dimer [Tegoni, Ramoni, Bignetti, Spinelli and Cambillau (1996) Nat. Struct. Biol. 3, 863-867; Bianchet, Bains, Petosi, Pevsner, Snyder, Monaco Amzel 934-939]. This contrasts with all known mammalian OBPs, which are monomers, in particular porcine OBP (OBPp), sharing 42.3% identity OBPb. By mechanism of swapping, monomers proposed to evolve into dimers oligomers, as observed human prion. Comparison...
The structure of bovine odorant‐binding protein (bOBP) revealed a striking feature dimer formed by domain swapping [Tegoni, M., Ramoni, R., Bignetti, E., Spinelli, S. & Cambillau, C. (1996) Nat. Struct. Biol. 3 , 863–867; Bianchet, M.A., Bains, G., Pelosi, P., Pevsner, J., Snyder, S.H., Monaco, H.L. Amzel, L.M. 934–939] and the presence naturally occuring ligand [Ramoni, Vincent, F., Grolli, S., Conti, V., Malosse, C., Boyer, F.D., Nagnan‐Le Meillour, Tegoni, M. (2001) J. Chem. 276...
Mesenchymal Stromal Cells (MSCs)-based therapies are rapidly gaining interest in veterinary medicine. Cellular therapy represents a new challenge for practitioners and requires precise coordination between the cell processing laboratory clinic. Cryopreservation is best method to provide fast, in-time, long-distance delivery of cells therapeutic applications. However, potentially toxic cryoprotectants xenobiotic products make direct administration impracticable patients. Alternatively, may be...
The present study aimed to detect microplastics in feline placentas and fetuses the early stage of pregnancy. For this study, 8 pregnant queens were evaluated. A standardized protocol for digestion biological matter was used, as well a plastic-free approach sample collection manipulation. Microplastics investigated by means Raman spectroscopy, with aim identifying their composition. Four eight animals contaminated, total 19 detected both fetal placental samples. Specifically, from cats 4 7...
Odorant binding proteins (OBP) are soluble lipocalins produced in large amounts the nasal mucosa of several mammalian species. Although OBPs can bind a variety odorous compounds, direct and exclusive involvement these olfactory perception has not been clearly demonstrated. This study investigated properties chemical resistance OBP to chemically reactive lipid peroxidation end‐product 4‐hydroxy‐2‐nonenal (HNE), an attempt establish functional relationship between this protein molecular...
The detection of hazard exposure is a current priority, including the traces explosive molecules in different environments like luggage storage rooms and public places, becoming major requirement for homeland security. In present study we carried out preliminary investigation on binding capacities four forms lipocalin odorant-binding protein (OBP) components such as diphenylamine, dimethyl-phthalate, resorcinol dinitrotoluene. experimental results, showing that OBP binds these compounds with...
Plastic is an important environmental issue and a more critical aspect concerns plastic fragments, mainly in term of nanoplastics (NPs). We demonstrated that NPs interfere with reproductive adipose stromal cells. Since several research underlined increased cardiovascular risk due to NPs, present study was undertaken investigate their effect on aortic endothelial cells (AOC). explored the specificity interaction cells, quantifying load treated Then, assessed cell growth, generation free...
ABSTRACT The complete genomic sequence of the dairy Lactobacillus helveticus bacteriophage ΦAQ113 was determined. Phage is a Myoviridae with an isometric capsid and contractile tail. final assembled consensus revealed linear, circularly permuted, double-stranded DNA genome size 36,566 bp G+C content 37%. Fifty-six open reading frames (ORFs) were predicted, putative function assigned to approximately 90% them. shows functionally related genes clustered together in structure composed modules...
Several studies have demonstrated that the endocrine disruptor bisphenol A (BPA) negatively affects animal and human health. An angiogenic process has been suggested among events disrupted by this molecule, but underlying mechanisms not yet clarified. The effect of BPA on angiogenesis was investigated means a bioassay previously validated in our laboratory. Using immortalized swine aortic endothelial cell line (AOC), development new blood vessels through three-dimensional vitro assay...
The bacterium Pseudomonas aeruginosa (PA) and the yeast Candida albicans (CA) are pathogens that cohabit mucosa of respiratory tracts animals humans. Their virulence is largely determined by chemical communication driven quorum sensing systems (QS), cross perception their molecules (QSM) can modulate prevalence one microorganism over other. Aiming to investigate whether some protein components dissolved in mucus layering might interfere with cross-communication these, eventually other...