A5020692656- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- Cardiac Fibrosis and Remodeling
- Radiopharmaceutical Chemistry and Applications
- Osteoarthritis Treatment and Mechanisms
- Pain Mechanisms and Treatments
- Pain Management and Placebo Effect
- Cardiomyopathy and Myosin Studies
- Hereditary Neurological Disorders
- Laser and Thermal Forming Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Ion channel regulation and function
- CAR-T cell therapy research
- Circular RNAs in diseases
- Viral Infections and Immunology Research
- Coronary Interventions and Diagnostics
- Diabetes Management and Research
- Cell Adhesion Molecules Research
- Additive Manufacturing Materials and Processes
- Protease and Inhibitor Mechanisms
- Extracellular vesicles in disease
- Nerve injury and regeneration
- Endoplasmic Reticulum Stress and Disease
Merck (Germany)
2023
Sanofi (Germany)
2005-2018
Fraunhofer Institute for Machine Tools and Forming Technology
2017
Ludwig-Maximilians-Universität München
2005
Kiel University
2005
Sanofi (France)
2005
Goethe University Frankfurt
2001
University Hospital of Bern
2000
Heidelberg University
1997-1998
Atrial fibrillation is associated with increased expression of ventricular myosin isoforms in atrial myocardium, regarded as part a dedifferentiation process. Whether reexpression restricted to transcripts encoding for contractile proteins unknown. Therefore, this study compares mRNA patients permanent sinus rhythm and gene using Affymetrix U133 arrays. In we identified 1434 genes deregulated fibrillation, the majority which, including key elements calcium-dependent signaling pathways,...
Chronic neuropathic pain is affected by specifics of the precipitating neural pathology, psychosocial factors, and genetic predisposition. Little known about identity predisposing genes. Using an integrative approach, we discovered that CACNG2 significantly affects susceptibility to chronic following nerve injury. encodes for stargazin, a protein intimately involved in trafficking glutamatergic AMPA receptors. The might also be Ca 2+ channel subunit. has previously been implicated epilepsy....
We investigated several hsp70/hsc70 interacting proteins and established by two independent techniques that hsp40 Hop/p60 specifically interact with the 257 residue carboxy‐terminal domain of hsp70 while Hap‐46 Hip/p48 bind 383 amino‐terminal ATP binding domain. competed for to hsc70, had no effect on either or hsc70. inhibited refolding thermally denatured firefly luciferase in an hsc70 dependent assay, this was largely compensated Hop/p60. These thus appear cooperate affecting chaperoning...
Background Nerve injury-triggered hyperexcitability in primary sensory neurons is considered a major source of chronic neuropathic pain. The hyperexcitability, turn, thought to be related transcriptional switching afferent cell somata. Analysis using expression microarrays has revealed that many genes are regulated the dorsal root ganglion (DRG) following axotomy. But which contribute pain phenotype versus other nerve injury-evoked processes such as regeneration? Using L5 spinal ligation...
The folding of protein structures often requires the presence molecular chaperones and/or chaperonin complexes. We here investigated inhibitory effects chaperone cofactors Hop/p60 and Hap46. By coimmunoprecipitation, we observed a direct interaction eukaryotic chaperonin-containing TCP-1 (CCT) purified from rabbit reticulocyte lysate with Hop/p60. contrast, Hap46 was not coprecipitated. Binding to CCT is dependent on ATP or ADP occurs through carboxyl-terminal sequences significantly...
To develop and compare three copper 64 ((64)Cu)-labeled antibody fragments derived from a CA6-targeting (huDS6) as immuno-positron emission tomography (immuno-PET)-based companion diagnostic agents for an antibody-drug conjugate by using huDS6.Three huDS6 were produced, purified, conjugated to 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), evaluated in the following ways: (a) affinity of DOTA conjugates was measured via flow cytometry, (b) stability labeled determined ex...
Several structurally divergent proteins associate with molecular chaperones of the 70-kDa heat shock protein (hsp70) family and modulate their activities. We investigated cofactors Hap46 Hop/p60 effects binding to mammalian hsp70 cognate form hsc70. associates amino-terminal ATP domain stimulates two- threefold but inhibits misfolded substrate hsc70 reactivation thermally denatured luciferase in an hsc70-dependent refolding system. By contrast, interacts a portion carboxy-terminal hsp70s,...
The aims of this study were to 1. define the responses glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP), glucagon, and peptide YY (PYY) an oral meal intravenous L-arginine; 2. examine correlation enteroendocrine hormones with insulin secretion. We hypothesized a relationship between circulating incretin concentrations secretion.Subjects normal glucose tolerance (NGT, n = 23), prediabetes (PDM, 17), or type 2 diabetes (T2DM, 22) studied twice, following...
Osteoarthritis is the most common degenerative disorder of modern world. However, many basic cellular features and molecular processes disease are poorly understood. In present study we used oligonucleotide-based microarray analysis genes known or assumed relevance to phenotype screen for relevant differences in gene expression between normal osteoarthritic chondrocytes. Custom made oligonucleotide DNA arrays were differentially expressed (n = 9) 10) cartilage samples. Real-time polymerase...
Introduction: Risk management and mitigation of new drug candidates that induce drug-induced liver injury (DILI) in the clinic is a pivotal task to protect patients save money during development process. Nevertheless, clinical DILI risk assessment with conventionally available biomarkers comes high failure rates. MicroRNAs (miRNAs) are short noncoding RNA molecules known regulate mRNA expression. Actively secreted miRNAs which circulating patient blood can provide important pathway...
A retroviral gene trap containing a human CD2 cell surface antigen/neomycin–phosphotransferase fusion in the U3 region of its LTR (U3Ceo) was used to screen mammalian genome for genes encoding secreted and/or transmembrane proteins that are repressed by oncogenic transformation. From an integration library consisting cells transformable insulin-like growth factor 1 (IGF-1), collection neomycin resistant (Neo R ) clones obtained; 86% also expressed antigen. Molecular analysis random sample...
11006 Background: SAR566658 is an antibody-drug immunoconjugate consisting of a humanized monoclonal antibody (huDS6) against the tumor-associated MUC1-sialoglycotope, CA6, conjugated to cytotoxic maytansinoid (DM4). currently undergoing phase I clinical trials in patients with CA6-positive solid tumors. A companion diagnostic based on huDS6 may facilitate patient stratification and early evaluation therapeutic efficacy. The present study describes development preclinical three novel...