A5076312725- Blood properties and coagulation
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Protease and Inhibitor Mechanisms
- Erythrocyte Function and Pathophysiology
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Proteins in Food Systems
- Blood Coagulation and Thrombosis Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Plant Reproductive Biology
- Neuropeptides and Animal Physiology
- Plant Molecular Biology Research
- Cell Adhesion Molecules Research
- Biochemical and Structural Characterization
- Sperm and Testicular Function
- Biotin and Related Studies
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Polyamine Metabolism and Applications
- Chemical Synthesis and Analysis
- Protein purification and stability
- Fibroblast Growth Factor Research
Max Planck Institute for Developmental Biology
2013-2023
Max Planck Institute of Biochemistry
1983-2015
Stony Brook University
1989-2009
Max Planck Society
1978-2009
University Medical Center
2009
Goethe University Frankfurt
2009
Johannes Gutenberg University Mainz
2009
University of California, Irvine
1991-2008
UC Irvine Health
2007
RELX Group (Netherlands)
1993
The amyloid A4 or beta peptide is a major component of extracellular deposits that are characteristic feature Alzheimer's disease. We synthesized series analogs the A4/beta which progressively longer at their carboxyl termini, including 42- and 39-residue peptides represent forms in senile plaque hereditary cerebral hemorrhage with amyloidosis form, respectively. All tested, 1-28 through 1-42, formed amyloid-like fibrils previously unreported thin sheet-like structures stained thioflavin T...
A new family of cysteine-rich antimicrobial peptides from bovine neutrophils was isolated and characterized.Thirteen structurally homologous were purified to homogeneity a granule-rich cytoplasmic fraction blood neutrophils.The complete sequences the determined by combination enzymatic digestion, Edman degradation, additional biochemical characterization carboxyl termini.The are characterized highly cationic 38-42-residue chain which includes 6 invariantly spaced cysteines form three...
A material which displayed opioid activity in the guinea pig ileum longitudinal muscle-myenteric plexus preparation was extracted from an enzymatic casein digest into chloroform/methanol. The extract roughly purified by adsorption/desorption procedures using charcoal and Amberlite XAD-2 resin as adsorbents. high degree of purity achieved high-pressure liquid chromatography on muBondapak C18 mu-Porasil columns finally gel filtration a Bio-Gel P-2 column. Several pronase-resistant compounds...
Five intestinal defensins, termed cryptdins 1-5, have been purified from mouse small bowel, sequenced, and localized to the epithelium by immunohistochemistry. Although identified as members of defensin peptide family sequencing, enteric defensins are novel in that four amino termini which three six residues longer than those leukocyte-derived defensins. A fifth cryptdin is first diverge previously invariant spacing cysteines structure. The most abundant defensin, cryptdin-1, had...
Integrins are cell-surface heterodimers formed by the association of one alpha- and beta-subunit. Glycoprotein IIIa (GPIIIa or beta 3 subunit) is common beta-subunit subfamily integrins, which, when associated with glycoprotein IIb (GPIIb), constitutes receptor for fibrinogen other adhesive proteins at platelet surface (the GPIIb-IIIa complex) and, alpha v-subunit, vitronectin present in several cell types. Protein chemical analysis GPIIIa allows us to define following structural domains:...
Two N-terminal fragments of α(A)-chain and β(B)-chain in human fibrinogen have been isolated from a plasmic hydrolyzate. The fragment the consisted 43 amino acid residues including two half-cystine residues. On treatment with thrombin, this produced other peptides addition to fibrinopeptide A its analogues. One was tripeptide, Gly-Pro-Arg, peptide containing 24 having valine. partial sequence has found be:...
The complete amino acid sequence of sauvagine, a new active polypeptide from the skin Phyllomedusa sauvagei , frog Central and South America, has been determined by automated liquid‐phase procedure after specific removal N ‐terminal pyrrolidonecarboxylic acid, cleavages at single methionine two arginine residues. proposed is: Pyr‐Gly‐Pro‐Pro‐Ile‐Ser‐Ile‐Asp‐Leu‐Ser‐Leu‐Glu‐Leu‐Leu‐Arg‐Lys‐Met‐Ile‐GIu‐Ile‐Glu‐Lys‐Gln‐Glu‐Lys‐Glu‐Lys‐Gln‐Gln‐Ala‐Ala‐Asn‐Asn‐Arg‐Leu‐Leu‐Leu‐Asp‐Thr‐Ile‐NH 2 ....
The optical brightener SCRI Renaissance 2200 can be used as versatile dye to study various aspects of plant reproduction by confocal laser scanning microscopy. sexual plants has traditionally relied on light microscopy in combination with a variety staining methods. Transgenic lines that label specific cell or tissue types fluorescent proteins were an important development visualize gametophyte development, the fertilization process, and follow differentiation early embryo. Staining...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRole of the .alpha.C Domains Fibrin in Clot FormationOleg V. Gorkun, Yuri I. Veklich, Leonid Medved', Agnes H. Henschen, and John W. WeiselCite this: Biochemistry 1994, 33, 22, 6986–6997Publication Date (Print):June 7, 1994Publication History Published online1 May 2002Published inissue 7 June 1994https://doi.org/10.1021/bi00188a031RIGHTS & PERMISSIONSArticle Views292Altmetric-Citations132LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant...
Platelet basic protein (PBP) was purified from the supernatant of thrombin-stimulated, washed human platelets by ion-exchange, affinity, molecular sieve, and high-performance liquid chromatography (HPLC). The NH2-terminal amino acid sequence determined automated Edman degradation, revealing 9 unique residues followed 10 established low-affinity platelet factor 4/beta-thromboglobulin (LA-PF4/beta TG) sequence. Among nine were three residues, accounting for high isoelectric point PBP....
The major fucose‐binding protein of 53 kDa from boar spermatozoa was isolated to apparent homogeneity using a two‐step procedure including high‐performance gel filtration and reversed‐phase chromatography. N‐terminal sequence the revealed that it is identical with sperm proteinase acrosin. By means solid‐phase zona‐binding assay based on avidin‐biotin system demonstrated acrosin also interacts strongly porcine zona pellucida. Thus, molecule combines specific proteolytic activity zona‐...
The two protamines of human sperm cell nuclei, P1 and P2, were isolated in pure form after extraction with 6M guanidine/5% mercaptoethanol alkylation vinyl pyridine by reversed-phase high-performance liquid chromatography. amino-acid sequence protamine was determined analysing the intact protein fragments obtained cyanogen bromide cleavage. Out 50 residues 24 are arginines 6 cysteines. P2 resulting from cleavage endoproteinase Lys-C thermolysin. Protamine found to occur forms which only...
In multicellular organisms, cellular differences in gene activity are a prerequisite for differentiation and establishment of cell types. order to study transcriptome profiles, specific types have be isolated from given tissue or even the whole organism. However, whole-transcriptome analysis early embryos flowering plants has been hampered by their size inaccessibility. Here, we describe purification nuclear RNA stage Arabidopsis thaliana using fluorescence-activated sorting (FANS) generate...
Our early study indicates that intracellular Aβ1–42 aggregates are resistant to degradation and accumulate as an insoluble residue in lysosomes, where they alter the normal catabolism of amyloid precursor protein (APP) cause accumulation APP amyloidogenic fragments. In this study, we examined whether addition exogenous also leads newly synthesized Aβ. Here describe Aβ, especially Aβn-42, is generated from metabolically labeled accumulates fraction cell lysates after treatment. These results...
The major fucose-binding protein of 53 kDa was isolated from boar spermatozoa by mild detergent extraction and subsequent high-performance gel filtration reversed-phase liquid chromatography. This has been identified as high-molecular-mass acrosin N-terminal sequencing. Treatment the with diisopropyl fluorophosphate abolishes enzymatic activity but not zona pellucida- properties. Mercaptolysis S-pyridyl-ethylation native two-chain followed HPLC SDS-PAGE revealed that binding properties are...